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Volumn 116, Issue , 2013, Pages 468-473
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Label-free fluorescent detection of thrombin activity based on a recombinant enhanced green fluorescence protein and nickel ions immobilized nitrilotriacetic acid-coated magnetic nanoparticles
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Author keywords
Fluorescence assay; Magnetic nanoparticles; Recombinant enhanced green fluorescent protein; Thrombin
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Indexed keywords
ENZYMES;
FLUORESCENCE;
MAGNETIC SEPARATION;
NANOPARTICLES;
RECOMBINANT PROTEINS;
REUSABILITY;
ENHANCED GREEN FLUORESCENCE PROTEINS;
ENHANCED GREEN FLUORESCENT PROTEIN;
FLUORESCENCE ASSAY;
FLUORESCENT DETECTION;
MAGNETIC NANO-PARTICLES;
NITRILOTRIACETIC ACID;
OPTIMIZED CONDITIONS;
THROMBIN;
NANOMAGNETICS;
ANTITHROMBIN;
DRUG DERIVATIVE;
ENHANCED GREEN FLUORESCENT PROTEIN;
GREEN FLUORESCENT PROTEIN;
HIRUDIN DERIVATIVE;
HIS HIS HIS HIS HIS HIS;
HIS-HIS-HIS-HIS-HIS-HIS;
HISTIDINE;
HYBRID PROTEIN;
IMMOBILIZED PROTEIN;
MAGNETIC NANOPARTICLE;
MAGNETITE NANOPARTICLE;
NITRILOTRIACETATE NICKEL;
NITRILOTRIACETIC ACID;
OLIGOPEPTIDE;
ORGANOMETALLIC COMPOUND;
THROMBIN;
ARTICLE;
BLOOD;
CHEMISTRY;
FLUORESCENCE ANALYSIS;
GENETICS;
HUMAN;
LIMIT OF DETECTION;
METHODOLOGY;
PROTEIN DEGRADATION;
RECOMBINANT ENHANCED GREEN FLUORESCENT PROTEIN;
RECYCLING;
REPRODUCIBILITY;
SPECTROFLUOROMETRY;
FLUORESCENCE ASSAY;
MAGNETIC NANOPARTICLES;
RECOMBINANT ENHANCED GREEN FLUORESCENT PROTEIN;
THROMBIN;
ANTITHROMBINS;
EQUIPMENT REUSE;
GREEN FLUORESCENT PROTEINS;
HIRUDINS;
HISTIDINE;
HUMANS;
IMMOBILIZED PROTEINS;
LIMIT OF DETECTION;
MAGNETITE NANOPARTICLES;
NITRILOTRIACETIC ACID;
OLIGOPEPTIDES;
ORGANOMETALLIC COMPOUNDS;
PROTEOLYSIS;
RECOMBINANT FUSION PROTEINS;
REPRODUCIBILITY OF RESULTS;
SPECTROMETRY, FLUORESCENCE;
THROMBIN;
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EID: 84881183616
PISSN: 00399140
EISSN: None
Source Type: Journal
DOI: 10.1016/j.talanta.2013.07.017 Document Type: Article |
Times cited : (13)
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References (40)
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