Cloning, overexpression, crystallization and preliminary X-ray crystallographic analysis of a slow-processing mutant of penicillin G acylase from Kluyvera citrophila

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 69, Issue 8, 2013, Pages 925-929

  Varshney, Nishant Kumar ^a   Ramasamy, Sureshkumar ^a,b   Brannigan, James A ^c   Wilkinson, Anthony J ^c   Suresh, C G ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

^b CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^c UNIVERSITY OF YORK   (United Kingdom)

Author keywords

autocatalytic processing; Kluyvera citrophila; pac gene; PGA precursor; spacer peptide

Indexed keywords

BACTERIAL PROTEIN; PENICILLIN AMIDASE;

ARTICLE; AUTOCATALYTIC PROCESSING; BIOSYNTHESIS; CHEMISTRY; CRYSTALLIZATION; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; KLUYVERA; KLUYVERA CRYOCRESCENS; METHODOLOGY; MOLECULAR CLONING; MUTATION; PAC GENE; PGA PRECURSOR; PROTEIN FOLDING; SPACER PEPTIDE; X RAY CRYSTALLOGRAPHY;

AUTOCATALYTIC PROCESSING; KLUYVERA CITROPHILA; PAC GENE; PGA PRECURSOR; SPACER PEPTIDE;

BACTERIAL PROTEINS; CLONING, MOLECULAR; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; GENE EXPRESSION REGULATION, BACTERIAL; KLUYVERA; MUTATION; PENICILLIN AMIDASE; PROTEIN FOLDING;

EID: 84881176267     PISSN: None     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S174430911301943X     Document Type: Article

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