Detection of necrosis by release of lactate dehydrogenase activity

Methods in Molecular Biology

Volumn 979, Issue , 2013, Pages 65-70

  Chan, Francis Ka Ming ^a,b   Moriwaki, Kenta ^a,b   De Rosa, María José ^a,b  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MASSACHUSETTS   (United States)

Author keywords

Lactate dehydrogenase; Membrane leakage; Necroptosis; Programmed necrosis

Indexed keywords

FORMAZAN; LACTATE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; TETRAZOLIUM;

APOPTOSIS; ARTICLE; CELL DEATH; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; COLORIMETRY; CONTROLLED STUDY; ENZYME ACTIVITY; NECROSIS; NONHUMAN; PRIORITY JOURNAL; TISSUE CULTURE; BIOCATALYSIS; CULTURE TECHNIQUE; ELECTRON TRANSPORT; ENZYMOLOGY; HUMAN; METABOLISM; METHODOLOGY; SECRETION (PROCESS);

BIOCATALYSIS; CELL CULTURE TECHNIQUES; COLORIMETRY; ELECTRON TRANSPORT; HUMANS; L-LACTATE DEHYDROGENASE; NAD; NECROSIS; TETRAZOLIUM SALTS;

MLCS; MLOWN;

EID: 84880949357     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-290-2_7     Document Type: Article

References (12)

1. Schweichel JU, Merker HJ (1973) The morphology of various types of cell death in prenatal tissues. Teratology 7:253-266
2. Moquin D, Chan FK (2010) The molecular regulation of programmed necrotic cell injury. Trends Biochem Sci 35:434-441
3. Challa S, Chan FK (2010) Going up in fl ames: necrotic cell injury and in fl ammatory diseases. Cell Mol Life Sci 67:3241-3253
4. Kono H, Rock KL (2008) How dying cells alert the immune system to danger. Nat Rev Immunol 8:279-289
5. Chipuk JE, Green DR (2005) Do inducers of apoptosis trigger caspase-independent cell death? Nat Rev Mol Cell Biol 6:268-275
6. Chan FK, Shisler J, Bixby JG, Felices M, Zheng L, Appel M, Orenstein J, Moss B, Lenardo MJ (2003) A role for tumor necrosis factor receptor- 2 and receptor-interacting protein in programmed necrosis and antiviral responses. J Biol Chem 278:51613-51621
7. Cho YS, Challa S, Moquin D, Genga R, Ray TD, Guildford M, Chan FK (2009) Phosphorylationdriven assembly of the RIP1-RIP3 complex regulates programmed necrosis and virusinduced in fl ammation. Cell 137:1112-1123
8. Holler N, Zaru R, Micheau O, Thome M, Attinger A, Valitutti S, Bodmer JL, Schneider P, Seed B, Tschopp J (2000) Fas triggers an alternative, caspase-8-independent cell death pathway using the kinase RIP as effector molecule. Nat Immunol 1:489-495
9. Burd JF, Usategui-Gomez M (1973) A colorimetric assay for serum lactate dehydrogenase. Clin Chim Acta 46:223-227
10. Korzeniewski C, Callewaert DM (1983) An enzyme-release assay for natural cytotoxicity. J Immunol Methods 64:313-320
11. Decker T, Lohmann-Matthes ML (1988) A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J Immunol Methods 115:61-69
12. Cory AH, Owen TC, Barltrop JA, Cory JG (1991) Use of an aqueous soluble tetrazolium/ formazan assay for cell growth assays in culture. Cancer Commun 3:207-212