A new approach of real time polymerase chain reaction in detection of vancomycin-resistant enterococci and its comparison with other methods

Indian Journal of Medical Microbiology

Volumn 31, Issue 1, 2013, Pages 47-52

  Tripathi, A ^a   Shukla, S K ^a   Singh, A ^a   Prasad, K N ^a  

^a SANJAY GANDHI POSTGRADUATE INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

Minimum inhibitory concentrations; real time polymerase chain reaction; vancomycin resistant enterococci

Indexed keywords

GLYCOPEPTIDE; PLASMID DNA; VANCOMYCIN;

AGAR DILUTION; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; COMPARATIVE STUDY; DNA EXTRACTION; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; GENOTYPE; IN VITRO STUDY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PATIENT CARE; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VANCOMYCIN RESISTANT ENTEROCOCCUS;

EID: 84880813538     PISSN: 02550857     EISSN: 19983646     Source Type: Journal    
DOI: 10.4103/0255-0857.108721     Document Type: Article

References (20)

1. Bonten MJ, Willems R, Weinstein RA. Vancomycin-resistant enterococci: Why are they here, and where do they come from? Lancet Infect Dis 2001;1:314-25.
2. Ghoshal U, Garg A, Tiwari DP, Ayyagari A. Emerging vancomycin resistance in enterococci in India. Indian J Pathol Microbiol 2006;49:620-2.
3. Moellering RC Jr. Emergence of Enterococcus as a signifi cant pathogen. Clin Infect Dis 1992;14:1173-6.
4. Courvalin P. Vancomycin resistance in Gram-positive cocci. Clin Infect Dis 2006;42:25-34.
5. Gold HS. Vancomycin-resistant enterococci: Mechanisms and clinical observations. Clin Infect Dis 2001;33:210-9.
6. Cetinkaya Y, Falk P, Mayhall CG. Vancomycin-resistant enterococci. Clin Microbiol Rev 2000;13:686-707.
7. Chen YS, Marshall SA, Winokur PL, Coffman SL, Wilke WW, Murray PR, et al. Use of molecular and reference susceptibility testing methods in a multicenter evaluation of MicroScan dried overnight Gram-positive MIC panels for detection of vancomycin and high-level aminoglycoside resistances in enterococci. J Clin Microbiol 1998;36:2996-3001.
8. Coombs GW, Kay ID, Steven RA, Pearman JW, Bertolatti D, Grubb WB. Should genotypic testing be done on all phenotypically vancomycin-resistant enterococci detected in hospitals? J Clin Microbiol 1999;37:1229-30.
9. Lee SY, Park KG, Lee GD, Park JJ, Park YJ. Comparison of Seeplex VRE detection kit with ChromID VRE agar for detection of vancomycin-resistant enterococci in rectal swab specimens. Ann Clin Lab Sci 2010;40:163-6.
10. Grabsch EA, Ghaly-Derias S, Gao W, Howden BP. Comparative study of selective chromogenic (chromID VRE) and bile esculin agars for isolation and identifi cation of vanB-containing vancomycin-resistant enterococci from feces and rectal swabs. J Clin Microbiol 2008;46:4034-6.
11. Peltroche-Llacsahuanga H, Top J, Weber-Heynemann J, Lütticken R, Haase G. Comparison of two chromogenic media for selective isolation of vancomycin-resistant enterococci from stool specimens. J Clin Microbiol 2009;47:4113-6.
12. Mak A, Miller MA, Chong G, Monczak Y. Comparison of PCR and culture for screening of vancomycin-resistant Enterococci: Highly disparate results for vanA and vanB. J Clin Microbiol 2009;47:4136-7.
13. Clinical and Laboratory Standard Institute-CLSI. Methods for Dilution Antimicrobial Susceptibility Test for Bacteria that Grow Aerobically; Approved standard-Seventh addition, 15th CLSI document M7-A7. Wayner, PA: CLSI/NCCLS; 2006 P 11-14.
14. Moritz EM, Hergenrother PJ. Toxin-antitoxin systems are ubiquitous and plasmid-encoded in vancomycin-resistant enterococci. Proc Natl Acad Sci U S A 2007;104:311-6.
15. Frahm E, Obst U. Application of the fl uorogenic probe technique (TaqMan PCR) to the detection of Enterococcus spp. and Escherichia coli in water samples. J Microbiol Methods 2003;52:123-31.
16. Asir K, Wilkinson K, Perry JD, Reed RH, Gould FK. Evaluation of chromogenic media for the isolation of vancomycin-resistant enterococci from stool samples. Lett Appl Microbiol 2009;48:230-3.
17. Delmas J, Robin F, Schweitzer C, Lesens O, Bonnet R. Evaluation of a new chromogenic medium, ChromID VRE, for detection of vancomycin-resistant enterococci in stool samples and rectal swabs. J Clin Microbiol 2007;45:2731-3.
18. Sloan LM, Uhl JR, Vetter EA, Schleck CD, Harmsen WS, Manahan J, et al. Comparison of the Roche LightCycler vanA/vanB detection assay and culture for detection of vancomycin-resistant enterococci from perianal swabs. J Clin Microbiol 2004;42:2636-43.
19. Dahl KH, Mater DD, Flores MJ, Johnsen PJ, Midtvedt T, Corthier G, et al. Transfer of plasmid and chromosomal glycopeptide resistance determinants occurs more readily in the digestive tract of mice than in vitro and exconjugants can persist stably in vivo in the absence of glycopeptide selection. J Antimicrob Chemother 2007;59:478-86.
20. Hammond DS, Schooneveldt JM, Nimmo GR, Huygens F, Giffard PM. bla (SHV) Genes in Klebsiella pneumoniae: Different allele distributions are associated with different promoters within individual isolates. Antimicrob Agents Chemother 2005;49:256-63.