hESC Expansion and Stemness Are Independent of Connexin Forty-Three-Mediated Intercellular Communication between hESCs and hASC Feeder Cells

PLoS ONE

Volumn 8, Issue 7, 2013, Pages

  Kim, Jin Su ^a   Kwon, Daekee ^a   Hwang, Seung Taeh ^a   Lee, Dong Ryul ^a   Shim, Sung Han ^a   Kim, Hee Chun ^b   Park, Hansoo ^c   Kim, Won ^d   Han, Myung Kwan ^e   Lee, Soo Hong ^a  

^a CHA University   (South Korea)

^b Bundang CHA Hospital   (South Korea)

^c Chung Ang University   (South Korea)

^d Jeonbuk National University Medical School and Hospital   (South Korea)

^e Chonbuk National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; CONNEXIN 43; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2;

ADIPOSE DERIVED STEM CELL; ARTICLE; CELL COMMUNICATION; CELL EXPANSION; CELL FUNCTION; CELL PROLIFERATION; CELL STRUCTURE; COLONY FORMATION; CONTROLLED STUDY; DOWN REGULATION; EMBRYONIC STEM CELL; FEEDER CELL; GAP JUNCTION; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; NUCLEOTIDE SEQUENCE; STEMNESS;

ADIPOSE TISSUE; CELL COMMUNICATION; CELL LINE; CELL PROLIFERATION; CONNEXIN 43; DOWN-REGULATION; EMBRYONIC STEM CELLS; EXTRACELLULAR SPACE; FEEDER CELLS; GAP JUNCTIONS; HUMANS; RNA, SMALL INTERFERING;

EID: 84880807250     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0069175     Document Type: Article

References (52)

1. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282: 1145-1147.
2. Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A, (2000) Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol 18: 399-404.
3. Xu X, Browning VL, Odorico JS, (2011) Activin, BMP and FGF pathways cooperate to promote endoderm and pancreatic lineage cell differentiation from human embryonic stem cells. Mech Dev 128: 412-427.
4. Liang YJ, Yang BC, Chen JM, Lin YH, Huang CL, et al. (2011) Changes in glycosphingolipid composition during differentiation of human embryonic stem cells to ectodermal or endodermal lineages. Stem Cells 29: 1995-2004.
5. Yabut O, Bernstein HS, (2011) The promise of human embryonic stem cells in aging-associated diseases. Aging (Albany NY) 3: 494-508.
6. Hentze H, Graichen R, Colman A, (2007) Cell therapy and the safety of embryonic stem cell-derived grafts. Trends Biotechnol 25: 24-32.
7. Xu C, Inokuma MS, Denham J, Golds K, Kundu P, et al. (2001) Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 19: 971-974.
8. Pakzad M, Totonchi M, Taei A, Seifinejad A, Hassani SN, et al. (2010) Presence of a ROCK inhibitor in extracellular matrix supports more undifferentiated growth of feeder-free human embryonic and induced pluripotent stem cells upon passaging. Stem Cell Rev 6: 96-107.
9. Amit M, Laevsky I, Miropolsky Y, Shariki K, Peri M, et al. (2011) Dynamic suspension culture for scalable expansion of undifferentiated human pluripotent stem cells. Nat Protoc 6: 572-579.
10. Miyazaki T, Futaki S, Hasegawa K, Kawasaki M, Sanzen N, et al. (2008) Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells. Biochem Biophys Res Commun 375: 27-32.
11. Catalina P, Montes R, Ligero G, Sanchez L, de la Cueva T, et al. (2008) Human ESCs predisposition to karyotypic instability: Is a matter of culture adaptation or differential vulnerability among hESC lines due to inherent properties? Mol Cancer 7: 76.
12. Ramos-Mejia V, Bueno C, Roldan M, Sanchez L, Ligero G, et al. (2011) The Adaptation of Human Embryonic Stem Cells to Different Feeder-Free Culture Conditions is Accompanied by a Mitochondrial Response. Stem Cells Dev.
13. Steiner D, Khaner H, Cohen M, Even-Ram S, Gil Y, et al. (2010) Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension. Nat Biotechnol 28: 361-364.
14. Schwartz SD, Hubschman JP, Heilwell G, Franco-Cardenas V, Pan CK, et al. (2012) Embryonic stem cell trials for macular degeneration: a preliminary report. Lancet 379: 713-720.
15. Escobedo-Lucea C, Stojkovic M, (2010) Growth of human embryonic stem cells using derivates of human fibroblasts. Methods Mol Biol 584: 55-69.
16. Unger C, Felldin U, Nordenskjold A, Dilber MS, Hovatta O (2008) Derivation of human skin fibroblast lines for feeder cells of human embryonic stem cells. Curr Protoc Stem Cell Biol Chapter 1: Unit 1C 7.
17. Cheng L, Hammond H, Ye Z, Zhan X, Dravid G, (2003) Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture. Stem Cells 21: 131-142.
18. Dravid G, Hammond H, Cheng L, (2006) Culture of human embryonic stem cells on human and mouse feeder cells. Methods Mol Biol 331: 91-104.
19. Park Y, Lee SJ, Choi IY, Lee SR, Sung HJ, et al. (2010) The efficacy of human placenta as a source of the universal feeder in human and mouse pluripotent stem cell culture. Cell Reprogram 12: 315-328.
20. Park Y, Choi IY, Lee SJ, Lee SR, Sung HJ, et al. (2010) Undifferentiated propagation of the human embryonic stem cell lines, H1 and HSF6, on human placenta-derived feeder cells without basic fibroblast growth factor supplementation. Stem Cells Dev 19: 1713-1722.
21. Hwang ST, Kang SW, Lee SJ, Lee TH, Suh W, et al. (2010) The expansion of human ES and iPS cells on porous membranes and proliferating human adipose-derived feeder cells. Biomaterials 31: 8012-8021.
22. Hwang ST, Lee SJ, Kang SW, Lee SH, (2009) Human Embryonic Stem Cell Culture Technique on Mitomycin C-free Feeder cells Using Porous Membrane. Tissue Engineering and Regenerative Medicine 6: 1375-1380.
23. Archambeault DR, Yao HH, (2010) Activin A, a product of fetal Leydig cells, is a unique paracrine regulator of Sertoli cell proliferation and fetal testis cord expansion. Proc Natl Acad Sci U S A 107: 10526-10531.
24. Wang L, Schulz TC, Sherrer ES, Dauphin DS, Shin S, et al. (2007) Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling. Blood 110: 4111-4119.
25. Hayashi Y, Furue MK, Okamoto T, Ohnuma K, Myoishi Y, et al. (2007) Integrins regulate mouse embryonic stem cell self-renewal. Stem Cells 25: 3005-3015.
26. Ma W, Tavakoli T, Derby E, Serebryakova Y, Rao MS, et al. (2008) Cell-extracellular matrix interactions regulate neural differentiation of human embryonic stem cells. BMC Dev Biol 8: 90.
27. Franke WW, (2009) Discovering the molecular components of intercellular junctions - a historical view. Cold Spring Harb Perspect Biol 1: a003061.
28. Wong RC, Dottori M, Koh KL, Nguyen LT, Pera MF, et al. (2006) Gap junctions modulate apoptosis and colony growth of human embryonic stem cells maintained in a serum-free system. Biochem Biophys Res Commun 344: 181-188.
29. Li D, Zhou J, Wang L, Shin ME, Su P, et al. (2010) Integrated biochemical and mechanical signals regulate multifaceted human embryonic stem cell functions. J Cell Biol 191: 631-644.
30. Li L, Wang S, Jezierski A, Moalim-Nour L, Mohib K, et al. (2010) A unique interplay between Rap1 and E-cadherin in the endocytic pathway regulates self-renewal of human embryonic stem cells. Stem Cells 28: 247-257.
31. Carpenter MK, Rosler ES, Fisk GJ, Brandenberger R, Ares X, et al. (2004) Properties of four human embryonic stem cell lines maintained in a feeder-free culture system. Dev Dyn 229: 243-258.
32. Galat V, Malchenko S, Galat Y, Ishkin A, Nikolsky Y, et al. (2011) A Model of Early Human Embryonic Stem Cell Differentiation Reveals Inter- and Intracellular Changes on Transition to Squamous Epithelium. Stem Cells Dev.
33. Kumar NM, Gilula NB, (1996) The gap junction communication channel. Cell 84: 381-388.
34. Saez JC, Berthoud VM, Branes MC, Martinez AD, Beyer EC, (2003) Plasma membrane channels formed by connexins: their regulation and functions. Physiol Rev 83: 1359-1400.
35. Krysko DV, Leybaert L, Vandenabeele P, D'Herde K, (2005) Gap junctions and the propagation of cell survival and cell death signals. Apoptosis 10: 459-469.
36. Sohl G, Willecke K, (2003) An update on connexin genes and their nomenclature in mouse and man. Cell Commun Adhes 10: 173-180.
37. Wong RC, Pera MF, Pebay A, (2008) Role of gap junctions in embryonic and somatic stem cells. Stem Cell Rev 4: 283-292.
38. Kang SW, Kim JS, Park KS, Cha BH, Shim JH, et al. (2011) Surface modification with fibrin/hyaluronic acid hydrogel on solid-free form-based scaffolds followed by BMP-2 loading to enhance bone regeneration. Bone 48: 298-306.
39. Trosko JE, Chang CC, Wilson MR, Upham B, Hayashi T, et al. (2000) Gap junctions and the regulation of cellular functions of stem cells during development and differentiation. Methods 20: 245-264.
40. Sohl G, Willecke K, (2004) Gap junctions and the connexin protein family. Cardiovasc Res 62: 228-232.
41. Galat V, Malchenko S, Galat Y, Ishkin A, Nikolsky Y, et al. (2012) A model of early human embryonic stem cell differentiation reveals inter- and intracellular changes on transition to squamous epithelium. Stem Cells Dev 21: 1250-1263.
42. Hardy K, Warner A, Winston RM, Becker DL, (1996) Expression of intercellular junctions during preimplantation development of the human embryo. Mol Hum Reprod 2: 621-632.
43. Bhattacharya B, Miura T, Brandenberger R, Mejido J, Luo Y, et al. (2004) Gene expression in human embryonic stem cell lines: unique molecular signature. Blood 103: 2956-2964.
44. Lin TM, Chang HW, Wang KH, Kao AP, Chang CC, et al. (2007) Isolation and identification of mesenchymal stem cells from human lipoma tissue. Biochem Biophys Res Commun 361: 883-889.
45. Assou S, Le Carrour T, Tondeur S, Strom S, Gabelle A, et al. (2007) A meta-analysis of human embryonic stem cells transcriptome integrated into a web-based expression atlas. Stem Cells 25: 961-973.
46. Todorova MG, Soria B, Quesada I, (2008) Gap junctional intercellular communication is required to maintain embryonic stem cells in a non-differentiated and proliferative state. J Cell Physiol 214: 354-362.
47. Sharovskaya YY, Philonenko ES, Kiselev SL, Lagarkova MA (2012) De novo reestablishment of gap junctional intercellular communications during reprogramming to pluripotency and differentiation. Stem Cells Dev.
48. Haku K, Muramatsu T, Hara A, Kikuchi A, Hashimoto S, et al. (2011) Epithelial Cell Rests of Malassez Modulate Cell Proliferation, Differentiation and Apoptosis via Gap Junctional Communication under Mechanical Stretching in vitro. Bull Tokyo Dent Coll 52: 173-182.
49. Abbaci M, Barberi-Heyob M, Blondel W, Guillemin F, Didelon J (2008) Advantages and limitations of commonly used methods to assay the molecular permeability of gap junctional intercellular communication. Biotechniques 45: 33-52, 56-62.
50. Wong RC, Pebay A, Nguyen LT, Koh KL, Pera MF, (2004) Presence of functional gap junctions in human embryonic stem cells. Stem Cells 22: 883-889.
51. Valiunas V, Doronin S, Valiuniene L, Potapova I, Zuckerman J, et al. (2004) Human mesenchymal stem cells make cardiac connexins and form functional gap junctions. J Physiol 555: 617-626.
52. Huettner JE, Lu A, Qu Y, Wu Y, Kim M, et al. (2006) Gap junctions and connexon hemichannels in human embryonic stem cells. Stem Cells 24: 1654-1667.