Quantitation of chronic and acute treatment effects on neuronal network activity using image and signal analysis: Toward a high-content assay

Journal of Biomolecular Screening

Volumn 18, Issue 7, 2013, Pages 807-819

  Cornelissen, Frans ^a   Verstraelen, Peter ^b   Verbeke, Tobias ^c   Pintelon, Isabel ^b   Timmermans, Jean Pierre ^b   Nuydens, Rony ^a   Meert, Theo ^a  

^a JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

^b UNIVERSITY OF ANTWERP   (Belgium)

^c Open Analytics   (Belgium)

Author keywords

calcium imaging; high content screening; image and signal analysis; in vitro synaptogenesis; neuronal network activity; primary hippocampal culture

Indexed keywords

EGTAZIC ACID; NERVE GROWTH FACTOR; SEROTONIN;

ALGORITHM; ARTICLE; CELL CULTURE; IN VITRO STUDY; LONG TERM CARE; MOUSE; NERVE CELL NETWORK; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL; SIGNAL PROCESSING;

TETRA;

EID: 84880767690     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057113486518     Document Type: Article

References (26)

1. Bourne J., Harris K. M.. Do Thin Spines Learn to be Mushroom Spines That Remember?. Curr. Opin. Neurobiol. 2007 ; 17: 381-386
2. Buzsaki G. Rhythms of the Brain. Oxford University Press: Oxford, UK ; 2006:
3. Uhlhaas P. J., Singer W.. Abnormal Neural Oscillations and Synchrony in Schizophrenia. Nat. Rev. Neurosci. 2010 ; 11: 100-113
4. Eytan D., Marom S.. Dynamics and Effective Topology Underlying Synchronization in Networks of Cortical Neurons. J. Neurosci. 2006 ; 26: 8465-8476
5. Feller M. B.. Spontaneous Correlated Activity in Developing Neural Circuits. Neuron. 1999 ; 22: 653-656
6. Smetters D., Mayevska A., Yuste R.. Detecting Action Potentials in Neuronal Populations with Calcium Imaging. Methods. 1999 ; 18: 215-221
7. Cohen E., Ivenshitz M., Amor-Baroukh V., Greenberger V., Segal M.. Determinants of Spontaneous Activity in Networks of Cultured Hippocampus. Brain Res. 2008 ; 1235: 21-30
8. Ivenshitz M., Segal M.. Neuronal Density Determines Network Connectivity and Spontaneous Activity in Cultured Hippocampus. J. Neurophysiol. 2010 ; 104: 1052-1060
9. Martinoia S., Bonzano L., Chiappalone M., Tedesco M., Marcoli M., Maura G.. In Vitro Cortical Neuronal Networks as a New High-Sensitive System for Biosensing Applications. Biosens. Bioelectron. 2005 ; 20: 2071-2078
10. Bokde A., Ewers M.. Assessing Neuronal Networks: Understanding Alzheimer's Disease. Prog. Neurobiol. 2009 ; 89: 125-133
11. Melom J., Littleton J.. Synapse Development in Health and Disease. Curr. Opin. Genet. Dev. 2011 ; 21: 256-261
12. Van Op den Bosch J., Van Nassauw L., Van Marck E., Timmermans J. P.. Somatostatin Modulates Mast Cell-Induced Responses in Murine Spinal Neurons and Satellite Cells. Am. J. Physiol. Gastrointest. Liver Physiol. 2009 ; 297: G406 - G417
13. Pickering M., Pickering B. W., Murphy K. J., O'Connor J. J.. Discrimination of Cell Types in Mixed Cortical Culture Using Calcium Imaging: A Comparison to Immunocytochemical Labeling. J. Neurosci. Methods. 2008 ; 173: 27-33
14. Cornelissen F., Cik M., Gustin E.. Phaedra, a Protocol-Driven System for Analysis and Validation of High-Content Imaging and Flow Cytometry. J. Biomol. Screen. 2012 ; 17: 496-508
15. Dean P., Mascio L., Ow D., Sudar D., Mullikin J.. Proposed Standard for Image Cytometry Data Files. Cytometry. 1990 ; 11: 561-569
16. Maini R., Mehra S.. A Review on JPEG2000 Image Compression. Int. J. Comput. Appl. 2010 ; 9: 43-47
17. Millard B., Niepel M., Menden M., Muhlich J., Sorger P.. Adaptive Informatics for Multifactorial and High-Content Biological Data. Nat. Methods. 2011 ; 8: 487-492
18. Kolesnikov A. Java Drawing with Apache Batik. BrainySoftware: Markham, Canada ; 2007:
19. GarciaD.Variance Estimation. http://www.biomecardio.com/matlab/evar.html (accessed Feb 13, 2013).
20. Savitzky A., Golay M. J. E.. Smoothing and Differentiation of Data by Simplified Least Squares Procedures. Anal. Chem. 1964 ; 36: 1627-1639
21. Leininger E., Belousov A.. Recovery of Network-Driven Glutamatergic Activity in Rat Hippocampal Neurons during Chronic Glutamate Receptor Blockade. Brain Res. 2009 ; 1251: 87-102
22. Horton J., Kleinman K. Using R for Data Management, Statistical Analysis and Graphics. New York: CRC Press ; 2011: 93-118.
23. Schmitz D., Empson R., Heinemann U.. Serotonin Reduces Inhibition via 5HT Receptors in Area CA1 of Rat Hippocampal Slices In Vitro. J. Neurosci. 1995 ; 15: 7217-7225
24. Hogberg H. T., Sobanski T.. Application of Micro-Electrode Arrays (MEAs) as an Emerging Technology for Developmental Neurotoxicity: Evaluation of Domoic Acid-Induced Effects in Primary Cultures of Rat Cortical Neurons. Neurotoxicology. 2011 ; 32: 158-168
25. Peterka D. S., Takahashi H.. Imaging Voltage in Neurons. Neuron. 2011 ; 69: 9-21
26. Horikawa K., Yamada Y.. Spontaneous Network Activity Visualized by Ultrasensitive Ca(2+) Indicators, Yellow Cameleon-Nano. Nat. Methods. 2010 ; 7: 729-732