A rapid method for infectivity titration of Andes hantavirus using flow cytometry

Journal of Virological Methods

Volumn 193, Issue 2, 2013, Pages 291-294

  Barriga, Gonzalo P ^a   Martínez Valdebenito, Constanza ^b   Galeno, Héctor ^c   Ferrés, Marcela ^b   Lozach, Pierre Yves ^d   Tischler, Nicole D ^a,e  

^a Fundacion Ciencia para la Vida   (Chile)

^b PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

^c INSTITUTO DE SALUD PÚBLICA DE CHILE   (Chile)

^d INRS INSTITUT ARMAND FRAPPIER   (Canada)

^e UNIVERSIDAD ANDRÉS BELLO   (Chile)

Author keywords

Flow cytometry; Focus assay; Hantavirus; Infectious virus titration; Nucleoprotein

Indexed keywords

MONOCLONAL ANTIBODY; NUCLEOCAPSID PROTEIN;

ACIDIFICATION; ARTICLE; CONTROLLED STUDY; ENDOSOME; FLOW CYTOMETRY; HANTAVIRUS; HANTAVIRUS INFECTION; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SENSITIVITY ANALYSIS; SERUM; VERO CELL; VIRUS ENTRY; VIRUS INFECTIVITY; VIRUS RELEASE; VIRUS TITRATION;

FLOW CYTOMETRY; FOCUS ASSAY; HANTAVIRUS; INFECTIOUS VIRUS TITRATION; NUCLEOPROTEIN;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODIES, VIRAL; CERCOPITHECUS AETHIOPS; FLOW CYTOMETRY; HANTAVIRUS; MICROBIAL VIABILITY; NUCLEOCAPSID PROTEINS; SENSITIVITY AND SPECIFICITY; TIME FACTORS; VERO CELLS; VIRAL LOAD;

EID: 84880675422     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.06.022     Document Type: Article

References (29)

1. Alexeyev O.A., Elgh F., Ahlm C., Stigbrand T., Settergren B., Wadell G., Juto P. Hantavirus antigen detection using human serum immunoglobulin M as the capturing antibody in an enzyme-linked immunosorbent assay. Am. J. Trop. Med. Hyg. 1996, 54:367-371.
2. Bharadwaj M., Nofchissey R., Goade D., Koster F., Hjelle B. Humoral immune responses in the hantavirus cardiopulmonary syndrome. J. Infect. Dis. 2000, 182:43-48.
3. Cifuentes-Muñoz N., Darlix J.L., Tischler N.D. Development of a lentiviral vector system to study the role of the Andes virus glycoproteins. Virus Res. 2010, 153:29-35.
4. Drayman N., Oppenheim A. Rapid titration of viruses by flow cytometry. Curr. Protoc. Cell Biol. 2011, 51:26.11.1-26.11.7.
5. Elliott R.M. Molecular biology of the Bunyaviridae. J. Gen. Virol. 1990, 71:501-522.
6. Ferres M., Vial P., Marco C., Yanez L., Godoy P., Castillo C., Hjelle B., Delgado I., Lee S.J., Mertz G.J. Prospective evaluation of household contacts of persons with hantavirus cardiopulmonary syndrome in Chile. J. Infect. Dis. 2007, 195:1563-1571.
7. Galeno H., Mora J., Villagra E., Fernandez J., Hernandez J., Mertz G.J., Ramirez E. First human isolate of Hantavirus (Andes virus) in the Americas. Emerg. Infect. Dis. 2002, 8:657-661.
8. Godoy P., Marsac D., Stefas E., Ferrer P., Tischler N.D., Pino K., Ramdohr P., Vial P., Valenzuela P.D., Ferres M., Veas F., Lopez-Lastra M. Andes virus antigens are shed in urine of patients with acute hantavirus cardiopulmonary syndrome. J. Virol. 2009, 83:5046-5055.
9. Hacker J.K., Hardy J.L. Adsorptive endocytosis of California encephalitis virus into mosquito and mammalian cells: a role for G1. Virology 1997, 235:40-47.
10. Heider H., Ziaja B., Priemer C., Lundkvist A., Neyts J., Kruger D.H., Ulrich R. A chemiluminescence detection method of hantaviral antigens in neutralisation assays and inhibitor studies. J. Virol. Methods 2001, 96:17-23.
11. Hooper J.W., Larsen T., Custer D.M., Schmaljohn C.S. A lethal disease model for hantavirus pulmonary syndrome. Virology 2001, 289:6-14.
12. Jin M., Park J., Lee S., Park B., Shin J., Song K.J., Ahn T.I., Hwang S.Y., Ahn B.Y., Ahn K. Hantaan virus enters cells by clathrin-dependent receptor-mediated endocytosis. Virology 2002, 294:60-69.
13. Krautkrämer E., Zeier M., Plyusnin A. Hantavirus infection: an emerging infectious disease causing acute renal failure. Kidney Int. 2013, 83:23-27.
14. Lozach P.Y., Mancini R., Bitto D., Meier R., Oestereich L., Overby A.K., Pettersson R.F., Helenius A. Entry of bunyaviruses into mammalian cells. Cell Host Microbe 2010, 7:488-499.
15. Macneil A., Nichol S.T., Spiropoulou C.F. Hantavirus pulmonary syndrome. Virus Res. 2011, 162:138-147.
16. Martinez V.P., Bellomo C., San Juan J., Pinna D., Forlenza R., Elder M., Padula P.J. Person-to-person transmission of Andes virus. Emerg. Infect. Dis. 2005, 11:1848-1853.
17. McCormick J.B., Sasso D.R., Palmer E.L., Kiley M.P. Morphological identification of the agent of Korean haemorrhagic fever (Hantaan virus) as a member of the Bunyaviridae. Lancet 1982, 1:765-768.
18. Niklasson B., Jonsson M., Lundkvist A., Horling J., Tkachenko E. Comparison of European isolates of viruses causing hemorrhagic fever with renal syndrome by a neutralization test. Am. J. Trop. Med. Hyg. 1991, 45:660-665.
19. Padula P.J., Edelstein A., Miguel S.D., Lopez N.M., Rossi C.M., Rabinovich R.D. Hantavirus pulmonary syndrome outbreak in Argentina: molecular evidence for person-to-person transmission of Andes virus. Virology 1998, 241:323-330.
20. Pekosz A., Griot C., Nathanson N., Gonzalez-Scarano F. Tropism of bunyaviruses: evidence for a G1 glycoprotein-mediated entry pathway common to the California serogroup. Virology 1995, 214:339-348.
21. Ramanathan H.N., Chung D.H., Plane S.J., Sztul E., Chu Y.K., Guttieri M.C., McDowell M., Ali G., Jonsson C.B. Dynein-dependent transport of the hantaan virus nucleocapsid protein to the endoplasmic reticulum-Golgi intermediate compartment. J. Virol. 2007, 81:8634-8647.
22. Ray N., Whidby J., Stewart S., Hooper J.W., Bertolotti-Ciarlet A. Study of Andes virus entry and neutralization using a pseudovirion system. J. Virol. Methods 2010, 163:416-423.
23. Saluzzo J.F., Leguenno B., Van der Groen G. Use of heat inactivated viral haemorrhagic fever antigens in serological assays. J. Virol. Methods 1988, 22:165-172.
24. Schmaljohn C., Hooper J. Bunyaviridae: the viruses and their replication. Fields Virology 2001, vol. 2. Lippincott Williams & Wilkins, Philadelphia, PA. 4th ed.
25. Schmaljohn C., Hjelle B. Hantaviruses: a global disease problem. Emerg. Infect. Dis. 1997, 3:95-104.
26. Tanishita O., Takahashi Y., Okuno Y., Yamanishi K., Takahashi M. Evaluation of focus reduction neutralization test with peroxidase-antiperoxidase staining technique for hemorrhagic fever with renal syndrome virus. J. Clin. Microbiol. 1984, 20:1213-1215.
27. Tischler N.D., Galeno H., Rosemblatt M., Valenzuela P.D. Human and rodent humoral immune responses to Andes virus structural proteins. Virology 2005, 334:319-326.
28. Tischler N.D., Rosemblatt M., Valenzuela P.D. Characterization of cross-reactive and serotype-specific epitopes on the nucleocapsid proteins of hantaviruses. Virus Res. 2008, 135:1-9.
29. Vera-Otarola J., Solis L., Soto-Rifo R., Ricci E.P., Pino K., Tischler N.D., Ohlmann T., Darlix J.L., Lopez-Lastra M. The Andes hantavirus NSs protein is expressed from the viral small mRNA by a leaky scanning mechanism. J. Virol. 2012, 86:2176-2187.