High-efficiency Agrobacterium-mediated transformation in Quercus robur: Selection by use of a temporary immersion system and assessment by quantitative PCR

Plant Cell, Tissue and Organ Culture

Volumn 114, Issue 2, 2013, Pages 171-185

  Mallon R ^a   Vieitez, A M ^a   Vidal, N ^a  

^a Santiago de Compostela   (Spain)

Author keywords

Improved genetic transformation; Pedunculate oak; Quantitative PCR; Temporary immersion; Transgene copy number

Indexed keywords

GENETIC TRANSFORMATION; PEDUNCULATE OAK; QUANTITATIVE PCR; TEMPORARY IMMERSIONS; TRANSGENE COPY NUMBERS;

PLANTS (BOTANY);

POLYMERASE CHAIN REACTION;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; BACTERIA (MICROORGANISMS); QUERCUS ROBUR; RHIZOBIUM;

EID: 84880634886     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-013-0313-3     Document Type: Article

References (55)

1. Álvarez R, Ordás RJ (2007) Improved genetic transformation protocol for cork oak (Quercus suber L.). Plant Cell, Tissue Organ Cult 91: 45-52. doi: 10. 1007/s11240-007-9276-6.
2. Álvarez R, Álvarez JM, Humara JM, Revilla A, Ordás RJ (2009) Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance. Biotechnol Lett 31: 1477-1483. doi: 10. 1007/s10529-009-0033-2.
3. Andrade GM, Nairn CJ, Le HT, Merkle SA (2009) Sexually mature transgenic American chestnut trees via embryogenic suspension-based transformation. Plant Cell Rep 28: 1385-1397. doi: 10. 1007/s00299-009-0738-7.
4. Arya M, Shergill IS, Williamson M, Gommersall L, Arya N, Patel HR (2005) Basic principles of real-time quantitative PCR. Expert Rev Mol Diagn 5: 209-219.
5. Bubner B, Baldwin IT (2004) Use of real-time PCR for determining copy number and zygosity in transgenic plants. Plant Cell Rep 23: 263-271. doi: 10. 1007/s00299-004-0859-y.
6. Casu RE, Selivanova A, Perroux JM (2012) High-throughput assessment of transgene copy number in sugarcane using real-time quantitative PCR. Plant Cell Rep 31: 167-177. doi: 10. 1007/s00299-011-1150-7.
7. Cervera M, Navarro A, Navarro L, Peña L (2008) Production of transgenic adult plants from clementine mandarin by enhancing cell competence for transformation and regeneration. Tree Physiol 28: 55-66. doi: 10. 1093/treephys/28. 155.
8. Corredoira E, Valladares S, Allona I, Aragoncillo C, Vieitez AM, Ballester A (2012) Genetic transformation of European chestnut somatic embryos with a native thaumatin-like protein (CsTL1) gene isolated from Castanea sativa seeds. Tree Physiol 32: 1389-1402. doi: 10. 1093/treephys/tps098.
9. Cruz-Hernandez A, Witjaksono Litz RE, Gomez Lim M (1998) Agrobacteriumtumefaciens-mediated transformation of embryogenic avocado cultures and regeneration of somatic embryos. Plant Cell Rep 17: 497-503.
10. Cuenca B, San-José MC, Martínez MT, Ballester A, Vieitez AM (1999) Somatic embryogenesis from stem and leaf explant of Quercus robur L. Plant Cell Rep 18: 538-543. doi: 10. 1007/s002990050618.
11. Du N, Pijut PM (2009) Agrobacterium-mediated transformation of Fraxinus pennsylvanica hypocotyls and plant regeneration. Plant Cell Rep 28: 915-923. doi: 10. 1007/s00299-009-0697-z.
12. Dutt M, Grosser JW (2010) An embryogenic suspension cell culture system for Agrobacterium-mediated transformation of citrus. Plant Cell Rep 29: 1251-1260. doi: 10. 1007/s00299-010-0910-0.
13. Espinosa P, Lorenzo JC, Iglesias A, Yabor L, Menéndez E, Borroto J, Hernández L, Arencibia AD (2002) Production of pinneaple transgenic plants assisted by temporary immersion bioreactors. Plant Cell Rep 21: 136-140. doi: 10. 1007/s00299-002-0481-9.
14. Etienne H, Berthouly M (2002) Temporary immersion systems in plant micropropagation. Plant Cell, Tissue Organ Cult 69: 215-231. doi: 10. 1023/A: 1015668610465.
15. Flachowsky H, Riedel M, Reim S, Hanke V (2008) Evaluation of the uniformity and stability of T-DNA integration and gene expression in transgenic apple plants. Electron J Biotechnol 11: 1-15. doi: 10. 2225/vol11-issue1-fulltext-10.
16. Gelvin SB (2003) Agrobacterium-mediated plant transformation: the biology behind the "Gene-Jockeying" tool. Microbiol Mol Biol Rev 67: 16-37. doi: 10. 1128/MMBR. 67. 1. 16-37. 2003.
17. Ginzinger DG (2002) Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol 30: 503-512.
18. Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum. Planta 107: 161-170.
19. Hajji M, Dreyer E, Marçais B (2009) Impact of Erysiphe alphitoides on transpiration and photosynthesis in Quercus robur leaves. Eur J Plant Pathol 125: 63-72. doi: 10. 1007/s10658-009-9458-7.
20. Hanhineva KJ, Kärenlampi SO (2007) Production of transgenic strawberries by temporary immersion bioreactor system and verification by TAIL-PCR. BMC Biotechnol 7: 11. doi: 10. 1186/1472-6750-7-11.
21. Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5: 387-405. doi: 10. 1007/BF02667740.
22. Jung T, Blaschke H, Oßwald W (2000) Involvement of soilborne Phytophthora species in Central European oak decline and the effect of site factors on the disease. Plant Pathol 49: 706-718. doi: 10. 1046/j. 1365-3059. 2000. 00521. x.
23. Kung Y-J, Yu T-A, Huang C-H, Wang H-C, Wang S-L, Yeh S-D (2010) Generation of hermaphrodite transgenic papaya lines with virus resistance via transformation of somatic embryos derived from adventitious roots of in vitro shoots. Transgenic Res 19: 621-635. doi: 10. 1007/s11248-009-9344-2.
24. Le VQ, Belles-Isles J, Dusabenyagasani M, Tremblay FM (2001) An improved procedure for production of white spruce (Picea glauca) transgenic plants using Agrobacterium tumefaciens. J Exp Bot 52: 2089-2095.
25. Leclercq J, Lardet L, Martin F, Chapuset T, Oliver G, Montoro P (2010) The green fluorescent protein as an efficient selection marker for Agrobacterium tumefaciens-mediated transformation in Hevea brasiliensis (Müll. Arg). Plant Cell Rep 29: 513-522. doi: 10. 1007/s00299-010-0840-x.
26. Mallón R, Covelo P, Vieitez AM (2012) Improving secondary embryogenesis in Quercus robur: application of temporary immersion for mass propagation. Trees 26: 731-741. doi: 10. 1007/s00468-011-0639-6.
27. Marchev A, Georgiev V, Ivanov I, Badjakov I, Pavlov A (2011) Two-phase temporary immersion system for Agrobacterium rhizogenes genetic transformation of sage (Salvia tomentosa Mill.). Biotechnol Lett 33: 1873-1878. doi: 10. 1007/s10529-011-0625-5.
28. Martínez T, Corredoira E, Valladares S, Jorquera L, Vieitez AM (2008) Germination and conversion of somatic embryos derived from mature Quercus robur trees: the effects of cold storage and thidiazuron. Plant Cell, Tissue Organ Cult 95: 341-351. doi: 10. 1007/s11240-008-9448-z.
29. Martínez MT, Vidal N, Ballester A, Vieitez AM (2012) Improved organogenic capacity of shoot cultures from mature pedunculate oak trees through somatic embryogenesis as a rejuvenation technique. Trees 26: 321-330. doi: 10. 1007/s00468-011-0594-2.
30. Mathews H, Litz RE, Wilde HD, Merkle SA, Wetzstein HY (1992) Stable integration and expression of β-glucuronidase and NPTII genes in mango somatic embryos. In Vitro Cell Dev Biol 28P: 172-178.
31. Matsunaga E, Nanto K, Oishi M, Ebinuma H, Morishita Y, Sakurai N, Suzuki H, Shibata D, Shimada T (2012) Agrobacterium-mediated transformation of Eucalyptus globulus using explants with shoot apex with introduction of bacterial choline oxidase gene to enhance salt tolerance. Plant Cell Rep 31: 225-235. doi: 10. 1007/s00299-011-1159-y.
32. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-497. doi: 10. 1111/j. 1399-3054. 1962. tb08052. x.
33. Nehra NS, Becwar MR, Rottmann WH, Pearson L, Chowdhury K, Chang S, Wilde HD, Kodrzycki RJ, Zhang C, Gause KC, Parks DW, Hinchee MA (2005) Forest biotechnology: innovative methods, emerging opportunities. In Vitro Cell Dev Biol Plant 41: 701-717. doi: 10. 1079/IVP2005691.
34. Padilla IMG, Burgos L (2010) Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols. Plant Cell Rep 29: 1203-1213. doi: 10. 1007/s00299-010-0900-2.
35. Palomo-Ríos E, Barceló-Muñoz A, Mercado JA, Pliego-Alfaro F (2012) Evaluation of key factors influencing Agrobacterium-mediated transformation of somatic embryos of avocado (Persea americana Mill.). Plant Cell, Tissue Organ Cult 109: 201-211. doi: 10. 1007/S11240-011-0086-5.
36. Pijut PM, Woest KE, Vengadesan G, Michler CH (2007) Technological advances in temperate hardwood tree improvement including breeding and molecular marker applications. In Vitro Cell Dev Biol Plant 43: 283-303. doi: 10. 1007/s11627-007-9026-9.
37. Raharjo SHT, Witjaksono NFN, Gomez-Lim MA, Padilla G, Litz RE (2008) Recovery of avocado (Persea americana Mill) plants transformed with the antifungal plant defensin gene PDF 12. In Vitro Cell Dev Biol Plant 44: 254-262. doi: 10. 1007/s11627-008-9117-2.
38. Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9: 30-33. doi: 10. 1007/BF00232130.
39. Sánchez C, Martínez TM, Vidal N, San-José MC, Valladares S, Vieitez AM (2008) Preservation of Quercus robur germplasm by cryostorage of embryogenic cultures derived from mature trees and RAPD analysis of genetic stability. Cryo Letters 29: 493-504.
40. San-José MC, Corredoira E, Martínez MT, Vidal N, Valladares S, Mallón R, Vieitez AM (2010) Shoot apex explants for induction of somatic embryogenesis in mature Quercus robur L. trees. Plant Cell Rep 29: 661-671. doi: 10. 1007/s00299-010-0852-6.
41. Schroeder H, Degen B (2008) Spatial genetic structure in populations of the green oak leaf roller, Tortrix viridiana L. (Lepidoptera, Tortricidae). Eur J For Res 127: 447-453. doi: 10. 1007/s10342-008-0228-4.
42. Shekhawat UKS, Ganapathi TR, Srinivas L, Bapat VA, Rathore TS (2008) Agrobacterium-mediated genetic transformation of embryogenic cell suspension cultures of Santalum album L. Plant Cell Tiss Organ Cult 92: 261-271. doi: 10. 1007/s11240-007-9330-4.
43. Thomas FM, Blank R, Hartmann G (2002) Abiotic and biotic factors and their interactions as causes of oak decline in central Europe. For Pathol 32: 277-307. doi: 10. 1046/j. 1439-0329. 2002. 00291. x.
44. Toribio M, Fernández C, Celestino C, Martínez MT, San-José MC, Vieitez AM (2004) Somatic embryogenesis in mature Quercus robur trees. Plant Cell, Tissue Organ Cult 76: 283-287. doi: 10. 1023/B: TICU. 0000009245. 92828. 26.
45. Torreblanca R, Cerezo S, Palomo-Ríos E, Mercado JA, Pliego-Alfaro F (2010) Development of a high throughput system for genetic transformation of olive (Olea europaea L.) plants. Plant Cell, Tissue Organ Cult 103: 61-69. doi: 10. 1007/s11240-010-9754-0.
46. Valladares S, Sánchez C, Martínez MT, Ballester A, Vieitez AM (2006) Plant regeneration through somatic embryogenesis from tissues of mature oak trees: true-to-type conformity of plantlets by RAPD analysis. Plant Cell Rep 25: 879-886. doi: 10. 1007/s00299-005-0108-z.
47. Varghese JM, Bhat AI (2011) An efficient Agrobacterium-mediated transformation protocol for black pepper (Piper nigrum L.) using embryogenic mass as explant. J Crop Sci Biotech 14: 247-254. doi: 10. 1007/s12892-011-0031-5.
48. Vidal N, Arellano G, San-José MC, Vieitez AM, Ballester A (2003) Developmental stages during the rooting of in vitro cultured Quercus robur shoots from material of juvenile and mature origin. Tree Physiol 23: 1247-1254. doi: 10. 1093/treephys/23. 18. 1247.
49. Vidal N, Mallón R, Valladares S, Meijomín AM, Vieitez AM (2010) Regeneration of transgenic plants by Agrobacterium-mediated transformation of somatic embryos of juvenile and mature Quercus robur. Plant Cell Rep 29: 1411-1422. doi: 10. 1007/s00299-010-0931-8.
50. Vieitez AM, Corredoira E, Martínez MT, San-José MC, Sánchez C, Valladares S, Vidal N, Ballester A (2012) Application of biotechnological tools to Quercus improvement. Eur J Forest Res 131: 519-539. doi: 10. 1007/s10342-011-0526-0.
51. Wang B, Zhang Z, Yin Z, Feng C, Wang Q (2012) Novel and potential application of cryopreservation to plant genetic transformation. Biotechnol Adv 30: 604-612. doi: 10. 1016/j. biotechadv. 2011. 10. 008.
52. Wang H, Petri C, Burgos L, Alburquerque N (2013) Phosphomannose-isomerase as a selectable marker for transgenic plum (Prunus domestica L.). Plant Cell Tissue Organ Cult. doi: 10. 1007/s11240-012-0259-x.
53. Watt MP (2012) The status of temporary immersion system (TIS) technology for plant micropropagation. Afr J Biotechn 11: 14025-14035. doi: 10. 5897/AJB12. 1693.
54. Yevtushenko DP, Misra S (2010) Efficient Agrobacterium-mediated transformation of commercial hybrid poplar Populus nigra L. x P. maximowiczii A. Henry Plant Cell Rep 29: 211-221. doi: 10. 1007/s00299-009-0806-z.
55. Zhang P, Li S-T, Liu T-T, Fu C-H, Zhou P-P, Zhao C-F, Yu L-J (2011) Overexpression of a 10-deacetylbaccatin III-10 β-O-acetyltransferase gene leads to increased taxol yield in cells of Taxus chinensis. Plant Cell, Tissue Organ Cult 106: 63-70. doi: 10. 1007/s11240-010-9894-2.