A role for immunohistochemical detection of BRAF V600E prior to BRAF-inhibitor treatment of malignant melanoma?

Journal of Clinical Pathology

Volumn 66, Issue 8, 2013, Pages 723-725

  Lade Keller, Johanne ^a   Kristensen, Lasse Sommer ^b   Riber Hansen, Rikke ^a   Guldberg, Per ^c   Hansen, Lise Lotte ^b   Steiniche, Torben ^a   Hager, Henrik ^a  

^a AARHUS UNIVERSITY HOSPITAL   (Denmark)

^b AARHUS UNIVERSITY   (Denmark)

^c DANISH CANCER SOCIETY RESEARCH CENTER   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

B RAF KINASE; B RAF KINASE INHIBITOR;

ARTICLE; CANCER TISSUE; GENE MUTATION; IMMUNOHISTOCHEMISTRY; MELANOMA; MUTATIONAL ANALYSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PYROSEQUENCING;

IMMUNOHISTOCHEMISTRY; MELANOMA; MOLECULAR PATHOLOGY; ONCOLOGY; SKIN TUMOURS;

HUMANS; IMMUNOHISTOCHEMISTRY; MELANOMA; MUTATION; PROTO-ONCOGENE PROTEINS B-RAF; SKIN NEOPLASMS;

EID: 84880587203     PISSN: 00219746     EISSN: 14724146     Source Type: Journal    
DOI: 10.1136/jclinpath-2013-201483     Document Type: Article

References (6)

1. Lade-Keller J, Rømer K, Guldberg P, et al. Evaluation of BRAF mutation testing methodologies in formalin fixed paraffin embedded cutaneous melanomas. J Mol Diagn 2013;15:70-80.
2. Long GV, Wilmott JS, Capper D, et al. Immunohistochemistry is highly sensitive and specific for the detection of V600E BRAF mutation in melanoma. Am J Surg Pathol 2013;37:61-5.
3. Pollock PM, Harper UL, Hansen KS, et al. High frequency of BRAF mutations in nevi. Nat Genet 2003;33:19-20.
4. Taube JM, Begum S, Shi C, et al. Benign nodal nevi frequently harbor the activating V600E BRAF mutation. Am J Surg Pathol 2009;33:568-71.
5. Halait H, Demartin K, Shah S, et al. Analytical performance of a real-time PCR-based assay for V600 mutations in the BRAF gene, used as the companion diagnostic test for the novel BRAF inhibitor vemurafenib in metastatic melanoma. Diagn Mol Pathol 2012;21:1-8.
6. Anderson S, Bloom KJ, Vallera DU, et al. Multisite analytic performance studies of a real-time polymerase chain reaction assay for the detection of BRAF V600E mutations in formalin-fixed paraffin-embedded tissue specimens of malignant melanoma. Arch Pathol Lab Med 2012.