Efficient genetic transformation of okra (Abelmoschus esculentus (L.) Moench) and generation of insect-resistant transgenic plants expressing the cry1Ac gene

Plant Cell Reports

Volumn 32, Issue 8, 2013, Pages 1191-1198

  Narendran, M ^a   Deole, Satish G ^a   Harkude, Satish ^a   Shirale, Dattatray ^a   Nanote, Asaram ^a   Bihani, Pankaj ^a   Parimi, Srinivas ^a   Char, Bharat R ^a   Zehr, Usha B ^a  

^a Maharashtra Hybrid Seed Company   (India)

Author keywords

Abelmoschus esculentus; Agrobacterium tumefaciens; Bt Bacillus thuringiensis; Shoot and fruit borer; Transgenic okra

Indexed keywords

BACTERIAL DNA; BACTERIAL PROTEIN; ENDOTOXIN; HEMOLYSIN; INSECTICIDAL CRYSTAL PROTEIN, BACILLUS THURINGIENSIS; T DNA; T-DNA;

ABELMOSCHUS; ANIMAL; ARTICLE; BIOASSAY; BIOLOGICAL PEST CONTROL; CHROMOSOME SEGREGATION; CROSS BREEDING; GENETIC TRANSFORMATION; GENETICS; INSECT; PARASITOLOGY; PHYSIOLOGY; SOUTHERN BLOTTING; TRANSGENIC PLANT;

ABELMOSCHUS; ANIMALS; BACTERIAL PROTEINS; BIOLOGICAL ASSAY; BLOTTING, SOUTHERN; CHROMOSOME SEGREGATION; CROSSES, GENETIC; DNA, BACTERIAL; ENDOTOXINS; HEMOLYSIN PROTEINS; INSECTS; PEST CONTROL, BIOLOGICAL; PLANTS, GENETICALLY MODIFIED; TRANSFORMATION, GENETIC;

ABELMOSCHUS; ABELMOSCHUS ESCULENTUS; AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; BACILLUS THURINGIENSIS; EARIAS VITTELLA; HEXAPODA; RHIZOBIUM;

EID: 84880506869     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-013-1415-4     Document Type: Article

References (32)

1. Ali M, Hossain MZ, Sarkern NC (2000) Inheritance of yellow vein mosaic virus (YVMV) tolerance in a cultivar of okra (Abelmoschus esculentus (L.) Moench). Euphytica 111: 205-209.
2. Brar KS, Arora SK, Ghai TR (1994) Losses in fruit yield of okra due to Earias spp. as influenced by dates of sowing and varieties. J Insect Sci 7: 133-135.
3. Dhankhar BS, Singh R, Kumar R, Kumar S (2009) Genetic improvement. In: Dhankhar BS, Singh R (eds) Okra handbook: global production, processing and crop improvement. HNB Publishing, New York, pp 125-158.
4. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11-15.
5. Düzyaman E, Vural H (2003) Evaluation of pod characteristics and nutritive value of okra genetic resources. Acta Hort (ISHS) 598: 103-110.
6. Fatokun CA (1987) Wide hybridization in okra. TAG Theor Appl Genet 74: 483-486.
7. Finer JJ, McMullen MD (1990) Transformation of cotton (Gossypium hirsutum L.) via particle bombardment. Plant Cell Rep 8: 586-590.
8. Fischhoff DA, Bowdish KS, Perlak FJ, Marrone PG, McCormick SM, Niedermeyer JG, Dean DA, Kusano-Kretzmer K, Mayer EJ, Rochester DE, Rogers SG, Fraley RT (1987) Insect tolerant transgenic tomato plants. Bio Tech 5: 807-812.
9. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirement of suspension cultures of soybean root cells. Exp Cell Res 50: 151-158.
10. Ganesan M, Chandrasekar R, Ranjitha Kumari BD, Jayabalan N (2007) Somatic embryogenesis and plant regeneration of okra (Abelmoschus esculentus (L.) Moench) through suspension culture. Biol Plant 51(3): 414-420.
11. Hamon S, van Sloten DH (1995) Okra. In: Smartt J, Simmonds NW (eds) Evolution of crop plants. Longman Scientific and Technical, Longman Group, UK.
12. Jambhale ND, Nerkar YS (1981) Inheritance of resistance to okra yellow vein mosaic disease in interspecific crosses of Abelmoschus. Theor Appl Genet 60: 313-316.
13. Joshi AB, Hardas MW (1956) Alloploid nature of okra, Abelmoschus esculentus (L.) Moench. Nature 178: 1190-1191.
14. Joshi AB, Singh HB, Joshi BS (1960) Is yellow vein mosaic disease a nuisance in your bhendi, then why not grow 'Pusa Sawani'. Indian Frm 10: 6-7.
15. Kay R, Chan A, Daly M, McPherson J (1987) Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes. Science 236: 1299-1303.
16. Kochko A, Hamon S (1990) A rapid and efficient method for the isolation of restrictable total DNA from plants of the genus Abelmoschus. Plant Mol Biol Rep 8: 3-7.
17. Krishnakumar NK, Srinivasan K (1987) Efficacy and economics of pest control of okra with conventional and synthetic pyrethroid insecticides. Indian J Plant Prot 15: 81-83.
18. Kumar PA, Sharma RP (1994) Genetic engineering of crop plants with Bacillus thuringiensis δ-endotoxin genes. J Plant Biochem Biotechnol 3: 3-9.
19. Kumar S, Dhingra A, Daniell H (2004) Stable transformation of the cotton plastid genome and maternal inheritance of transgenes. Plant Mol Biol 56: 203-216.
20. Mangat BS, Roy MK (1986) Tissue culture and plant regeneration of okra (Abelmoschus esculentus). Plant Sci 47: 57-61.
21. Memon AJ, Abro GH, Syed TS (2004) Varietal resistance of okra against Earias spp. J Entomol 1: 1-5.
22. Metz TD, Roush RT, Tang JD, Shelton AM, Earle ED (1995) Transgenic broccoli expressing a Bacillus thuringiensis insecticidal crystal protein: implications for pest resistance strategies. Mol Breed 1: 309-317.
23. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.
24. Nwangburuka CC, Kehinde OB, Adegbite OA, Denton OA (2011) Mitotic chromosomes in Abelmoschus esculentus (L.) Moench esculentus (L.) Moench. Ann Biol Res 2: 85-90.
25. Perlak FJ, Oppenhuizen M, Gustafson K, Voth R, Sivasupramaniam S, Heering D, Carey B, Ihrig RA, Roberts JK (2001) Development and commercial use of Bollgard cotton in the USA-early promises versus today's reality. Plant J. 27: 489-501.
26. Rajamony L, Chandran M, Rajmohan K (2006) In Vitro embryo rescues of interspecific crosses for transferring virus resistance in okra (Abelmoschus esculentus (L.) Moench). Acta Hort (ISHS) 725: 235-240.
27. Roy MK, Mangat BS (1989) Regeneration of plants from callus tissue of okra (Abelmoschus esculentus). Plant Sci 60: 77-81.
28. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 82-84.
29. Satpathy S, Rai S (1998) Influence of sowing date and crop phenology on pest infestation in okra. Veg Sci 25: 175-180.
30. Shukla A, Agrawal RK, Pathak SC (1997) Seasonal incidence of okra shoot and fruit borer, Earias vittella (Fab.) and effect of temperature on its infestation level. Adv Plant Sci 10: 169-172.
31. Sunilkumar G, Rathore KS (2001) Transgenic cotton: factors influencing Agrobacterium-mediated transformation and regeneration. Mol Breed 8: 37-52.
32. Verma RS, Upadhyay KD, Gupta SP, Singh R, Viswanath, Lodhi PS (1984) Interaction between root-knot nematode, Meloidogyne javanica and spotted bollworm. Earias vittella on okra and effects on yield. Indian J Plant Prot 12: 131-133.