Concentration-dependent specimen shrinkage in iodine-enhanced microCT

Journal of Anatomy

Volumn 223, Issue 2, 2013, Pages 185-193

  Vickerton, Paula ^a   Jarvis, Jonathan ^b   Jeffery, Nathan ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

^b LIVERPOOL JOHN MOORES UNIVERSITY   (United Kingdom)

Author keywords

Iodine; Iodine potassium iodide; MicroCT; Shrinkage; Stain

Indexed keywords

FORMALDEHYDE; IODINE;

ANIMAL TISSUE; ARTICLE; CEREBELLUM; CONCENTRATION RESPONSE; CONTRAST ENHANCEMENT; HEART MUSCLE; IMMERSION; MICRO-COMPUTED TOMOGRAPHY; MOUSE; NONHUMAN; PRIORITY JOURNAL; SKELETAL MUSCLE;

IODINE; IODINE POTASSIUM IODIDE; MICROCT; SHRINKAGE; STAIN;

ANIMALS; CEREBELLUM; CONTRAST MEDIA; FIXATIVES; HEART; IODINE COMPOUNDS; MICE; MUSCLE, SKELETAL; ORGAN SIZE; TISSUE FIXATION; X-RAY MICROTOMOGRAPHY;

EID: 84880176242     PISSN: 00218782     EISSN: 14697580     Source Type: Journal    
DOI: 10.1111/joa.12068     Document Type: Article

References (29)

1. Ameen M-U, Abedin N (1975) Anatomy, histology, and secretions of the salivary glands of Sphaerodema rusticum (F.) (Hemiptera: Belostomatidae). Int J Insect Morphol Embryol 4, 35-47.
2. Arborgh B, Bell P, Brunk U, et al. (1976) The osmotic effect of glutaraldehyde during fixation. A transmission electron microscopy, scanning electron microscopy and cytochemical study. J Ultrastruct Res 56, 339-350.
3. Aslanidi O, Nikolaidou T, Zhao J, et al. (2012) Application of micro-computed tomography with iodine staining to cardiac imaging, segmentation and computational model development. IEEE Trans Med Imaging 32, 8-17.
4. Bacallao R, Sohrab S, Phillips C (2006) Guiding principles of specimen preservation for confocal fluorescence microscopy. Handbook of Biological Confocal Microscopy, 368-380, New York, USA: Springer.
5. Baverstock H, Jeffery N, Cobb S (2013) The morphology of the mouse masticatory musculature. J Anat 223, 46-60.
6. Boyde A (2012) Staining plastic blocks with triiodide to image cells and soft tissues in backscattered electron SEM of skeletal and dental tissues. Eur Cells Mater 24, 154-161.
7. Boyde A, Franc F (1981) Freeze-drying shrinkage of glutaraldehyde fixed liver. J Microsc 122, 75-86.
8. Boyde A, Maconnachie E (1980) Treatment with lithium salts reduces ethanol dehydration shrinkage of glutaraldehyde fixed tissue. Histochemistry 66, 181-187.
9. Boyde A, Maconnachie E (1981) Morphological correlations with dimensional change during SEM specimen preparation. Scan Electron Microsc 4, 27.
10. Boyde A, Maconnachie E (1983) Not quite critical point drying. The Science of Biological Specimen Preparation. pp. 71-75, Chicago: Scanning electron microscopy Inc.
11. Cox PG, Jeffery N (2011) Reviewing the morphology of the jaw-closing musculature in squirrels, rats, and guinea pigs with contrast-enhanced microCt. Anat Rec 294, 915-928.
12. Cox PG, Fagan MJ, Rayfield EJ, et al. (2011) Finite element modelling of squirrel, guinea pig and rat skulls: using geometric morphometrics to assess sensitivity. J Anat 219, 696-709.
13. Cox PG, Rayfield EJ, Fagan MJ, et al. (2012) Functional evolution of the feeding system in Rodents. PLoS ONE 7, e36299.
14. Degenhardt K, Wright AC, Horng D, et al. (2010) Rapid 3D phenotyping of cardiovascular development in mouse embryos by micro-CT with iodine staining: clinical perspective. Circ Cardiovasc Imaging 3, 314-322.
15. Douglass JK, Wcislo WT (2010) An inexpensive and portable microvolumeter for rapid evaluation of biological samples. Biotechniques 49, 566.
16. Elliott JC, Dover SD (1982) X-ray microtomography. J Microsc 126, 211-213.
17. Fox CH, Johnson FB, Whiting J, et al. (1985) Formaldehyde fixation. J Histochem Cytochem 33, 845-853.
18. Gusnard D, Kirschner RH (1977) Cell and organelle shrinkage during preparation for scanning electron microscopy: effects of fixation, dehydration and critical point drying. J Microsc 110, 51-57.
19. Hammer Ø, Harper DA, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontologia electronica 4, 9.
20. Hautier L, Lebrun R, Cox PG (2012) Patterns of covariation in the masticatory apparatus of hystricognathous rodents: Implications for evolution and diversification. J Morphol 273, 1319-1337.
21. Hopwood D (1982) Fixation and fixatives. In: Theory and Practice of Histological Techniques. (eds Stevens A, Bancroft JD), pp. 20-40, New York: Churchill Livingstone.
22. Jeffery NS, Stephenson RS, Gallagher JA, et al. (2011) Micro-computed tomography with iodine staining resolves the arrangement of muscle fibres. J Biomech 44, 189-192.
23. Metscher BD (2009) MicroCT for developmental biology: a versatile tool for high-contrast 3D imaging at histological resolutions. Dev Dyn 238, 632-640.
24. Schmidt E, Parsons T, Jamniczky H, et al. (2010) Micro-computed tomography-based phenotypic approaches in embryology: procedural artifacts on assessments of embryonic craniofacial growth and development. BMC Dev Biol 10, 18.
25. Stephenson RS, Boyett MR, Hart G, et al. (2012) Contrast enhanced micro-computed tomography resolves the 3-dimensional morphology of the cardiac conduction system in mammalian hearts. PLoS ONE 7, e35299.
26. Tsai HP, Holliday CM (2011) Ontogeny of the alligator cartilago transiliens and its significance for sauropsid jaw muscle evolution. PLoS ONE 6, e24935.
27. Weisbecker V (2012) Distortion in formalin-fixed brains: using geometric morphometrics to quantify the worst-case scenario in mice, Brain Struct Funct 217, 677-685.
28. Wick MR (2012) Histochemistry as a tool in morphological analysis: a historical review. Ann Diagn Pathol 16, 71-78.
29. Young J (1935) Osmotic pressure of fixing solutions. Nature 135, 823-824.