Buffer-free production of gamma-aminobutyric acid using an engineered glutamate decarboxylase from Escherichia coli

Enzyme and Microbial Technology

Volumn 53, Issue 3, 2013, Pages 200-205

  Kang, Taek Jin ^a   Ho, Ngoc Anh Thu ^a   Pack, Seung Pil ^b  

^a Dongguk University   (South Korea)

^b Korea University   (South Korea)

Author keywords

Buffer free enzyme reaction; Enzyme engineering; Gamma aminobutyric acid; Glutamate decarboxylase

Indexed keywords

ACIDIC CONDITIONS; AMINOBUTYRIC ACIDS; DOWNSTREAM PURIFICATIONS; ENZYME ENGINEERING; ENZYME REACTION; GAMMA-AMINOBUTYRIC ACIDS; GLUTAMATE DECARBOXYLASE; MONOSODIUM SALTS;

CARBOXYLATION; ENZYMES; ESCHERICHIA COLI;

AMINO ACIDS;

4 AMINOBUTYRIC ACID; GLUTAMATE DECARBOXYLASE; MONOSODIUM SALT; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ARTICLE; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; PH; SYNTHESIS;

BUFFER-FREE ENZYME REACTION; ENZYME ENGINEERING; GAMMA-AMINOBUTYRIC ACID; GLUTAMATE DECARBOXYLASE;

BUFFERS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GAMMA-AMINOBUTYRIC ACID; GLUTAMATE DECARBOXYLASE; GLUTAMIC ACID; HYDROGEN-ION CONCENTRATION; KINETICS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SODIUM GLUTAMATE; WATER;

ESCHERICHIA COLI;

EID: 84880038125     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2013.04.006     Document Type: Article

References (19)

1. Wong C.G., Bottiglieri T., Snead O.C. GABA, gamma-hydroxybutyric acid, and neurological disease. Annals of Neurology 2003, 54:S3-S12.
2. Baum G., Lev-Yadun S., Fridmann Y., Arazi T., Katsnelson H., Zik M., et al. Calmodulin binding to glutamate decarboxylase is required for regulation of glutamate and GABA metabolism and normal development in plants. EMBO Journal 1996, 15:2988-2996.
3. Komatsuzaki N., Shima J., Kawamoto S., Momose H., Kimura T. Production of gamma-aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods. Food Microbiol 2005, 22:497-504.
4. Kawasaki N., Nakayama A., Yamano N., Takeda S., Kawata Y., Yamamoto N., et al. Synthesis, thermal and mechanical properties and biodegradation of branched polyamide 4. Polymer 2005, 46:9987-9993.
5. Ault A. The monosodium glutamate story: the commercial production of MSG and other amino acids. Journal of Chemical Education 2004, 81:347-355.
6. Camien M.N., McClure L.E., Lepp A., Dunn M.S. The preparation of gamma-aminobutyric acid by enzymatic decarboxylation of L-glutamic acid. Archives of Biochemistry and Biophysics 1953, 43:378-380.
7. Lammens T.M., De Biase D., Franssen M.C.R., Scott E.L., Sanders J.P.M. The application of glutamic acid alpha-decarboxylase for the valorization of glutamic acid. Green Chemistry 2009, 11:1562-1567.
8. Plokhov A.Y., Gusyatiner M.M., Yampolskaya T.A., Kaluzhsky V.E., Sukhareva B.S., Schulga A.A. Preparation of γ-aminobutyric acid using E. coli cells with high activity of glutamate decarboxylase. Applied Biochemistry and Biotechnology 2000, 88:257-265.
9. Le Vo T.D., Kim T.W., Hong S.H. Effects of glutamate decarboxylase and gamma-aminobutyric acid (GABA) transporter on the bioconversion of GABA in engineered Escherichia coli. Bioprocess and Biosystems Engineering 2012, 35:645-650.
10. Takahashi C., Shirakawa J., Tsuchidate T., Okai N., Hatada K., Nakayama H., et al. Robust production of gamma-amino butyric acid using recombinant Corynebacterium glutamicum expressing glutamate decarboxylase from Escherichia coli. Enzyme and Microbial Technology 2012, 51:171-176.
11. Ho N.A.T., Hou C.Y., Kim W.H., Kang T.J. Expanding the active pH range of Escherichia coli glutamate decarboxylase by breaking the cooperativeness. Journal of Bioscience and Bioengineering 2013, 115:154-158.
12. Foster J.W. Escherichia coli acid resistance: tales of an amateur acidophile. Nature Reviews Microbiology 2001, 2:898-907.
13. Shukuya R., Schwert G.W. Glutamic acid decarboxylase: I. Isolation procedures and properties of the enzyme. Journal of Biological Chemistry 1960, 235:1649-1652.
14. Gut H., Pennacchietti E., John R.A., Bossa F., Capitani G., De Biase D., et al. Escherichia coli acid resistance: pH-sensing, activation by chloride and autoinhibition in GadB. EMBO Journal 2006, 25:2643-2651.
15. Dutyshev D.I., Darii E.L., Fomenkova N.P., Nikonov S.V., Andreeva N.S., Sukhareva B.S. Structure of Escherichia coli glutamate decarboxylase (GADa) in complex with glutarate at 2.05Å. Acta Crystallographica D 2005, 61:230-235.
16. Capitani G., De Biase D., Aurizi C., Gut H., Bossa F., Grutter M.G. Crystal structure and functional analysis of Escherichia coli glutamate decarboxylase. EMBO Journal 2003, 22:4027-4037.
17. 465 alters the pH-dependent spectroscopic properties of Escherichia coli glutamate decarboxylase and broadens the range of its activity toward more alkaline pH. Journal of Biological Chemistry 2009, 284:31587-31596.
18. Ueno H. Enzymatic and structural aspects on glutamate decarboxylase. Journal of Molecular Catalysis B: Enzymatic 2000, 10:67-79.
19. Lammens T.M., Franssen M.C.R., Scott E.L., Sanders J.P.M. Synthesis of biobased N-methylpyrrolidone by one-pot cyclization and methylation of gamma-aminobutyric acid. Green Chemistry 2010, 12:1430-1436.