Validation of a cell-based ELISA as a screening tool identifying anti-alphavirus small-molecule inhibitors

Journal of Virological Methods

Volumn 193, Issue 1, 2013, Pages 226-231

  Spurgers, Kevin B ^a   Hurt, Clarence R ^b   Cohen, Jeffrey W ^a   Eccelston, Lori T ^a   Lind, Cathleen M ^a   Lingappa, Vishwanath R ^b   Glass, Pamela J ^a  

^a UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

^b Prosetta Antiviral Inc   (United States)

Author keywords

Alphavirus; ELISA; Screen; Small molecule inhibitor; Venezuelan equine encephalitis virus (VEEV); Virus yield reduction assay

Indexed keywords

ANTIVIRUS AGENT; SMALL MOLECULE INHIBITOR; UNCLASSIFIED DRUG;

ALPHA VIRUS; ANALYTICAL PARAMETERS; ANIMAL CELL; ANTIVIRAL ACTIVITY; ARTICLE; CELL ASSAY; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; CYTOTOXICITY; DRUG SCREENING; ENZYME LINKED IMMUNOSORBENT ASSAY; LABORATORY AUTOMATION; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SCALE UP; SIGNAL TO BACKGROUND RATIO; VALIDATION PROCESS; VENEZUELAN EQUINE ENCEPHALOMYELITIS ALPHAVIRUS; VERO CELL; VIRUS CELL INTERACTION; VIRUS EXAMINATION; VIRUS INHIBITION; VIRUS YIELD REDUCTION ASSAY; Z FACTOR;

50% INHIBITORY CONCENTRATION; ALPHAVIRUS; BOVINE SERUM ALBUMIN; BSA; CHIKUNGUNYA VIRUS; CHIKV; CPE; CYTOPATHIC EFFECT; EASTERN EQUINE ENCEPHALITIS VIRUS; EEEV; ELISA; ENZYME-LINKED IMMUNOSORBENT ASSAY; HORSERADISH PEROXIDASE; HRP; IC(50); MAB; MOI; MONOCLONAL ANTIBODY; MULTIPLICITY OF INFECTION; PBS; PFU; PHOSPHATE-BUFFERED SALINE; PLAQUE-FORMING UNITS; ROOM TEMPERATURE; RT; S/B; SCREEN; SD; SIGNAL TO BACKGROUND RATIO; SMALL-MOLECULE INHIBITOR; STANDARD DEVIATION; TCID(50); TISSUE CULTURE INFECTIOUS DOSE 50; TRD; TRINIDAD DONKEY STRAIN OF VEEV; UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASE; USAMRIID; VEEV; VENEZUELAN EQUINE ENCEPHALITIS VIRUS; VENEZUELAN EQUINE ENCEPHALITIS VIRUS (VEEV); VIRUS YIELD REDUCTION ASSAY; WEEV; WESTERN EQUINE ENCEPHALITIS VIRUS;

ALPHAVIRUS; ANIMALS; ANTIVIRAL AGENTS; CERCOPITHECUS AETHIOPS; DRUG EVALUATION, PRECLINICAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; VERO CELLS;

ALPHAVIRUS; VENEZUELAN EQUINE ENCEPHALITIS VIRUS; WESTERN EQUINE ENCEPHALOMYELITIS VIRUS;

EID: 84880014221     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.06.007     Document Type: Article

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