The use of fluorescent probes in the study of reactive oxygen species in pancreatic cancer cells

Methods in Molecular Biology

Volumn 980, Issue , 2013, Pages 321-329

  Dinnen, Richard D ^a   Mao, Yuehua ^a   Fine, Robert L ^a  

^a NONE

Author keywords

Fluorescent probes; Hydrogen peroxides; Hydroxyl radicals; Mutant K ras; Pancreatic cancer; Reactive oxygen species (ROS); Superoxide radicals

Indexed keywords

DICHLORODIHYDROFLUORESCEIN; DIHYDRORHODAMINE 123; FLUORESCEIN; FLUORESCENT DYE; HYDROETHIDINE; REACTIVE OXYGEN METABOLITE; SINGLET OXYGEN;

APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL DEATH; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENT LIGHTING; HUMAN; HUMAN CELL; LIGHT EXPOSURE; PANCREAS CANCER; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; METABOLISM; NECROSIS; PANCREAS TUMOR; STAINING; TUMOR CELL LINE;

APOPTOSIS; CELL LINE, TUMOR; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; NECROSIS; PANCREATIC NEOPLASMS; REACTIVE OXYGEN SPECIES; STAINING AND LABELING;

MLCS; MLOWN;

EID: 84879587880     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-287-2_18     Document Type: Article

References (13)

1. Raha S, Robinson BH (2001) Mitochondria, oxygen free radicals, and apoptosis. Am J Med Genet 106:62-70
2. Hileman EO, Liu J, Albitar M, Keating MJ, Huang P (2004) Intrinsic oxidative stress in cancer cells: a biochemical basis for therapeutic selectivity. Cancer Chemother Pharmacol 53:209-219
3. Maciag A, Anderson LM (2005) Reactive oxygen species and lung tumorigenesis by mutant K-ras: a working hypothesis. Exp Lung Res 31:83-104
4. Cullen JJ, Weydert C, Hinkhouse MM, Ritchie J, Domann FE, Spitz D, Oberley LW (2003) The role of manganese superoxide dismutase in the growth of pancreatic adenocarcinoma. Cancer Res 63:1297-1303
5. Halliwell B, Whiteman M (2004) Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?. Br J Pharmacol 142:231-255
6. Wright WE, Shay JW (2002) Historical claims and current interpretations of replicative aging. Nat Biotechnol 20:682-688
7. Buxser SE, Sawada G, Raub TJ (1999) Analytical and numerical techniques for evaluation of free radical damage in cultured cells using imaging cytometry and fluorescent indicators. Methods Enzymol 300:256-275
8. Zhao H, Kalivendi S, Zhang H, Joseph J, Nithipatikom K, Vasquez-Vivar J, Kalyanaraman B (2003) Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxide. Free Radic Biol Med 34: 1359-1368
9. Setsukinai K, Urano Y, Kakinuma K, Majima HJ, Nagano T (2003) Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish speci fic species. J Biol Chem 278:3170-3175
10. Castilho RF, Ward MW, Nicholls DG (1999) Oxidative stress, mitochondrial function, and acute glutamate excitotoxicity in cultured cerebellar granule cells. J Neurochem 72: 1394-1401
11. Flors C, Fryer MJ, Waring J, Reeder B, Bechtold U, Mullineaux PM, Nonell S, Wilson MT, Baker NR (2006) Imaging the production of singlet oxygen in vivo using a new fluorescent sensor, Singlet Oxygen Sensor Green. J Exp Bot 57:1725-1734
12. Mukhopadhyay P, Rajesh M, Yoshihiro K, Hasko G, Pacher P (2007) Simple quantitative detection of mitochondrial superoxide production in live cells. Biochem Biophys Res Commun 358:203-208
13. Jakubowski W, Bartosz G (2000) 2,7-dichloro fluorescin oxidation and reactive oxygen species: what does it measure?. Cell Biol Int 24:757-760