Increase of salt dependence of halophilic nucleoside diphosphate kinase caused by a single amino acid substitution

Extremophiles

Volumn 17, Issue 4, 2013, Pages 585-591

  Ishibashi, Matsujiro ^a   Hayashi, Tomoe ^a   Yoshida, Chiho ^a   Tokunaga, Masao ^a  

^a KAGOSHIMA UNIVERSITY   (Japan)

Author keywords

Folding; Halobacterium; Holophilic enzyme; Nucleoside diphosphate kinase; Optimum salt concentration; Protein engineering

Indexed keywords

ARCHAEA; HALOBACTERIUM; HALOBACTERIUM SALINARUM;

EID: 84879506459     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s00792-013-0541-9     Document Type: Article

References (23)

1. Amoozegar MA, Salehghamari E, Khajeh K, Kabiri M, Naddaf S (2008) Production of an extracellular thermohalophilic lipase from a moderately halophilic bacterium, Salinivibrio sp. strain SA-2. J Basic Microbiol 48: 160-167.
2. Arakawa T, Timasheff SN (1982) Preferential interactions of proteins with salts in concentrated solutions. Biochemistry 21: 6545-6552.
3. Besir H, Zeth K, Bracher A, Heider U, Ishibashi M, Tokunaga M, Oesterhelt D (2005) Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum. FEBS Lett 579: 6595-6600.
4. Fukushima TM, Echigo A, Inoue A, Usami R (2005) Organic solvent tolerance of halophilic alpha-amylase from a haloarchaeon, Haloarcula sp. strain S-1. Extremophiles 9: 85-89.
5. Ishibashi M, Tokunaga H, Hiratsuka K, Yonezawa Y, Tsurumaru H, Arakawa T, Tokunaga M (2001) NaCl-activated nucleoside diphosphate kinase from extremely halophilic archaeon, Halobacterium salinarum, maintains native conformation without salt. FEBS Lett 493: 134-138.
6. Ishibashi M, Arakawa T, Tokunaga M (2004) Facilitated folding and subunit assembly in Escherichia coli and in vitro of nucleoside diphosphate kinase from extremely halophilic archaeon conferred by amino-terminal extension containing hexa-His-tag. FEBS Lett 570: 87-92.
7. Ishibashi M, Tatsuda S, Izutsu K, Kumeda K, Arakawa T, Tokunaga M (2007) A single Gly114Arg mutation stabilizes the hexameric subunit assembly and changes the substrate specificity of halo-archaeal nucleoside diphosphate kinase. FEBS Lett 581: 4073-4079.
8. Ishibashi M, Iwasa T, Kumeda K, Arakawa T, Tokunaga M (2009) Effects of mutations at Gly114 on the stability and refolding of Haloarchaeal nucleoside diphosphate kinase in low salt solution. Int J Biol Macromol 44: 361-364.
9. Ishibashi M, Ida K, Tatsuda S, Arakawa T, Tokunaga M (2011) Interaction of hexa-His tag with acidic amino acids results in facilitated refolding of halophilic nucleoside diphosphate kinase. Int J Biol Macromol 49: 778-783.
10. Ishibashi M, Uchino M, Arai S, Kuroki R, Arakawa T, Tokunaga M (2012) Reduction of salt-requirement of halophilic nucleoside diphosphate kinase by engineering S-S bond. ABB 525: 47-52.
11. Kamekura M, Hamakawa T, Onishi H (1982) Application of halophilic nuclease H of Micrococcus varians subsp. halophilus to commercial production of flavoring agent 5-GMP. Appl Environ Microbiol 44: 994-995.
12. Kushner DJ (1978) Life in high salt and solute concentrations: halophilic bacteria. In: Kushuner DJ (ed) Microbial life in extreme environments. Academic Press, London, pp 317-368.
13. Kushner DJ (1985) The Halobacteriaceae. In the bacteria, vol. VIII. Academic Press, London, pp. 171-214.
14. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
15. Lanyi JK (1974) Salt-dependent properties of proteins from extremely halophilic bacteria. Bacteriol Rev 38: 272-290.
16. Malender W, Horvath C (1977) Salt effect on hydrophobic interactions in precipitation and chromatography of proteins: an interpretation of the lyotropic series. Arch Biochem Biophys 183: 200-215.
17. Mizuki T, Kamekura M, Ishibashi M, Usami R, Yoshida Y, Tokunaga M, Horikoshi K (2004) Nucleoside diphosphate kinase of halobacteria. Amino acid sequence and salt-response pattern. J Jpn Soc Extremophiles 3: 18-27.
18. Paul S, Bag SK, Das S, Harvill ET, Dutta C (2008) Molecular signature of hypersaline adaptation: insights from genome and proteome composition of halophilic prokaryotes. Genome Biol 9: R70.
19. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Measurement of protein using bicinchoninic acid. Anal Biochem 150: 76-85.
20. Studdert CA, Herrera Seitz MK, Plasencia Gil MI, Sanchez JJ, de Castro RE (2001) Purification and biochemical characterization of the haloalkaliphilic archaeon Natronococcus occultus extracellular serine protease. J Basic Microbiol 41: 375-383.
21. Tokunaga H, Ishibashi M, Arakawa T, Tokunaga M (2004) Highly efficient renaturation of beta-lactamase isolated from moderately halophilic bacteria. FEBS Lett 558: 7-12.
22. Tokunaga H, Saito S, Sakai K, Yamaguchi R, Katsuyama I, Arakawa T, Onozaki K, Tokunaga M (2010) Halophilic beta-lactamase as a new solubility- and folding-enhancing tag protein: production of native human interleukin 1alpha and human neutrophil alpha-defensin. Appl Microbiol Biotechnol 86: 649-658.
23. Yamaguchi R, Tokunaga H, Ishibashi M, Arakawa T, Tokunaga M (2011) Salt-dependent thermo-reversible α-amylase: cloning and characterization of halophilic α-amylase from moderately halophilic bacterium, Kocuria varians. Appl Microbiol Biotechnol 89: 673-684.