False positive RNA binding activities after Ni-affinity purification from Escherichia coli

RNA Biology

Volumn 10, Issue 6, 2013, Pages 1066-1069

  Milojevic, Tetyana ^a   Sonnleitner, Elisabeth ^a   Romeo, Alessandra ^a   Djinovic Carugo, Kristina ^a,b   Bläsi, Udo ^a  

^a UNIVERSITY OF VIENNA   (Austria)

^b UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

Crc; EMSA; Hfq; IMAC; Ni affinity purification; RNA binding proteins

Indexed keywords

BACTERIAL RNA; NITRILOTRIACETATE NICKEL; POLYHISTIDINE TAG;

BACTERIUM CONTAMINATION; BINDING AFFINITY; DEFENSE MECHANISM; ESCHERICHIA COLI; FALSE POSITIVE RESULT; GEL MOBILITY SHIFT ASSAY; GEL PERMEATION CHROMATOGRAPHY; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMUNODETECTION; NONHUMAN; NOTE; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PSEUDOMONAS AERUGINOSA; RNA BINDING;

ESCHERICHIA COLI;

EID: 84879494331     PISSN: 15476286     EISSN: 15558584     Source Type: Journal    
DOI: 10.4161/rna.25195     Document Type: Note

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