Spectrum of bacterial colonization associated with urothelial cells from patients with chronic lower urinary tract symptoms

Journal of Clinical Microbiology

Volumn 51, Issue 7, 2013, Pages 2054-2062

  Khasriya, Rajvinder ^a,d   Sathiananthamoorthy, Sanchutha ^a   Ismail, Salim ^b   Kelsey, Michael ^c   Wilson, Mike ^b   Rohn, Jennifer L ^a   Malone Lee, James ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

^c WHITTINGTON HOSPITAL NHS TRUST   (United Kingdom)

^d BARNET HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL COLONIZATION; BACTERIAL COUNT; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATE; COLONY FORMING UNIT; CONTROLLED STUDY; CYTOLYSIS; ENTEROCOCCUS FAECALIS; EPITHELIUM CELL; ESCHERICHIA COLI; FEMALE; HUMAN; LACTOBACILLUS GASSERI; LOWER URINARY TRACT SYMPTOM; MAJOR CLINICAL STUDY; MALE; NONHUMAN; PRIORITY JOURNAL; PROSPECTIVE STUDY; PROTEUS MIRABILIS; PYURIA; SEDIMENT; STREPTOCOCCUS ANGINOSUS; URINARY TRACT INFECTION; URINE CULTURE; ADULT; AGED; BACTERIA; BACTERIAL INFECTION; CHRONIC DISEASE; CLASSIFICATION; ISOLATION AND PURIFICATION; LOWER URINARY TRACT SYMPTOMS; MICROBIOLOGY; MIDDLE AGED; UROTHELIUM;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ADULT; AGED; BACTERIA; BACTERIAL INFECTIONS; CHRONIC DISEASE; FEMALE; HUMANS; LOWER URINARY TRACT SYMPTOMS; MALE; MIDDLE AGED; URINARY TRACT INFECTIONS; UROTHELIUM;

EID: 84879441873     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.03314-12     Document Type: Article

References (43)

1. American College of Obstetricians and Gynecologists. 2008. ACOG Practice Bulletin No. 91: treatment of urinary tract infections in nonpregnant women. Obstet. Gynecol. 111:785-794.
2. Scottish Intercollegiate Guidelines Network. 2012. Management of suspected bacterial urinary tract infection in adults: a national clinical guideline. Scottish Intercollegiate Guidelines Network, Edinburgh, Scotland.
3. Kass EH. 1957. Bacteriuria and the diagnosis of infection in the urinary tract. Arch. Intern. Med. 100:709-714.
4. Kass EH. 1960. Bacteriuria and pyelonephritis of pregnancy. Arch. Intern. Med. 105:194-198.
5. Bradley GM. 1968. Differentiating epithelial cells from leukocytes in urine. Postgrad. Med. 43:245-248.
6. Wear JB, Jr. 1966. Correlation of pyuria, stained urine smear, urine culture and the uroscreen test. J. Urol. 96:808-811.
7. Khasriya R, Khan S, Lunawat R, Bishara S, Bignal J, Malone-Lee M, Ishii H, O'Connor D, Kelsey M, Malone-Lee J. 2010. The inadequacy of urinary dipstick and microscopy as surrogate markers of urinary tract infection in urological outpatients with lower urinary tract symptoms without acute frequency and dysuria. J. Urol. 183:1843-1847.
8. Kline KA, Schwartz DJ, Gilbert NM, Hultgren SJ, Lewis AL. 2012. Immune modulation by group B Streptococcus influences host susceptibility to urinary tract infection by uropathogenic Escherichia coli. Infect. Immun. 80:4186-4194.
9. Stamm WE, Counts GW, Running KR, Fihn S, Turck M, Holmes KK. 1982. Diagnosis of coliform infection in acutely dysuric women. N. Engl. J. Med. 307:463-468.
10. Deville WL, Yzermans JC, van Duijn NP, Bezemer PD, van der Windt DA, Bouter LM. 2004. The urine dipstick test useful to rule out infections: a meta-analysis of the accuracy. BMC Urol. 4:4. doi:10.1186/1471-2490-4-4.
11. Hurlbut TA, III, Littenberg B. 1991. The diagnostic accuracy of rapid dipstick tests to predict urinary tract infection. Am. J. Clin. Pathol. 96: 582-588.
12. Williams GJ, Macaskill P, Chan SF, Turner RM, Hodson E, Craig JC. 2010. Absolute and relative accuracy of rapid urine tests for urinary tract infection in children: a meta-analysis. Lancet Infect. Dis. 10:240-250.
13. Colgan R, Williams M. 2011. Diagnosis and treatment of acute uncomplicated cystitis. Am. Fam. Physician 84:771-776.
14. Coyne KS, Sexton CC, Thompson CL, Milsom I, Irwin D, Kopp ZS, Chapple CR, Kaplan S, Tubaro A, Aiyer LP, Wein AJ. 2009. The prevalence of lower urinary tract symptoms (LUTS) in the USA, the UK and Sweden: results from the Epidemiology of LUTS (EpiLUTS) study. BJU Int. 104:352-360.
15. Irwin DE, Milsom I, Hunskaar S, Reilly K, Kopp Z, Herschorn S, Coyne K, Kelleher C, Hampel C, Artibani W, Abrams P. 2006. Populationbased survey of urinary incontinence, overactive bladder, and other lower urinary tract symptoms in five countries: results of the EPIC Study. Eur. Urol. 50:1306-1314.
16. Abrams P, Cardozo L, Fall M, Griffiths D, Rosier P, Ulmsten U, Van Kerrebroeck P, Victor A, Wein A. 2003. The standardisation of terminology in lower urinary tract function: report from the standardisation sub-committee of the International Continence Society. Urology 61:37-49.
17. Anderson GG, Palermo JJ, Schilling JD, Roth R, Heuser J, Hultgren SJ. 2003. Intracellular bacterial biofilm-like pods in urinary tract infections. Science 301:105-107.
18. Mysorekar IU, Hultgren SJ. 2006. Mechanisms of uropathogenic Escherichia coli persistence and eradication from the urinary tract. Proc. Natl. Acad. Sci. U. S. A. 103:14170-14175.
19. Rosen DA, Hooton TM, Stamm WE, Humphrey PA, Hultgren SJ. 2007. Detection of intracellular bacterial communities in human urinary tract infection. PLoS Med. 4:e329. doi:10.1371/journal.pmed.0040329.
20. Garofalo CK, Hooton TM, Martin SM, Stamm WE, Palermo JJ, Gordon JI, Hultgren SJ. 2007. Escherichia coli from urine of female patients with urinary tract infections is competent for intracellular bacterial community formation. Infect. Immun. 75:52-60.
21. Anderson GG, Martin SM, Hultgren SJ. 2004. Host subversion by formation of intracellular bacterial communities in the urinary tract. Microbes Infect. 6:1094-1101.
22. Avery K, Donovan J, Peters TJ, Shaw C, Gotoh M, Abrams P. 2004. ICIQ: a brief and robust measure for evaluating the symptoms and impact of urinary incontinence. Neurourol. Urodyn. 23:322-330.
23. Heuer RJ. 2006. Psychology of intelligence analysis. Novinka Books, New York, NY.
24. Al-Buheissi S, Khasriya R, Maraj BH, Malone-Lee J. 2008. A simple validated scale to measure urgency. J. Urol. 179:1000-1005.
25. Gill K, Kupelian A, Brackenridge L, Horsley H, Sathiananthamoorthy S, Malone-Lee J. 2011. Surprising symptoms indicating urinary tract infection. Abstr. 41st Annu. Meet. Int. Continence Soc., abstr. 444.
26. Kupelian A, Chaliha C, Gill K, Brackenridge L, Horsley H, Malone-Lee J. 2009. Pain symptoms as part of the OAB complex. Int. Urogynecol. J. 22:189-190.
27. Brown J, Meikle J, Webb C. 1991. Collecting midstream specimens of urine-the research base. Nurs. Times 87:49-52.
28. McGinley M, Wong LL, McBride JH, Rodgerson DO. 1992. Comparison of various methods for the enumeration of blood cells in urine. J. Clin. Lab. Anal. 6:359-361.
29. Horsley H, Tuz M, Collins L, Swamy S, Malone-Lee J, Rohn J. Investigating the origin of epithelial cells found in the urine of LUTS patients using immunofluorescence; contamination or inflammation? Int. Urogynecol. J., in press.
30. Khasriya R. 2011. Occult urine infection in the aetiology of the overactive bladder. Ph.D. thesis. University College London, London, United Kingdom.
31. Martinez JJ, Mulvey MA, Schilling JD, Pinkner JS, Hultgren SJ. 2000. Type 1 pilus-mediated bacterial invasion of bladder epithelial cells. EMBO J. 19:2803-2812.
32. Kupelian A, Horsley H, Khasriya R, Amussah R, Badiani R, Courtney A, Chandhyoke N, Riaz U, Savlani K, Moledina M, Montes S, O'Connor D, Visavadia R, Kelsey M, Rohn J, Malone-Lee J. 2013. Discrediting microscopic pyuria and leucocyte esterase as diagnostic surrogates for infection in patients with lower urinary tract symptoms: results from a clinical and laboratory evaluation. BJU Int. doi:10.1111/j.1464-410X.2012.11694.x.
33. Amna MA, Chazan B, Raz R, Edelstein H, Colodner R. 2013. Risk factors for non-Escherichia coli community-acquired bacteriuria. Infection 41:473-477.
34. Wolfe AJ, Toh E, Shibata N, Rong R, Kenton K, Fitzgerald M, Mueller ER, Schreckenberger P, Dong Q, Nelson DE, Brubaker L. 2012. Evidence of uncultivated bacteria in the adult female bladder. J. Clin. Microbiol. 50:1376-1383.
35. Salvador E, Wagenlehner F, Kohler CD, Mellmann A, Hacker J, Svanborg C, Dobrindt U. 2012. Comparison of asymptomatic bacteriuria Escherichia coli isolates from healthy individuals versus those from hospital patients shows that long-term bladder colonization selects for attenuated virulence phenotypes. Infect. Immun. 80:668-678.
36. Nobbs AH, Lamont RJ, Jenkinson HF. 2009. Streptococcus adherence and colonization. Microbiol. Mol. Biol. Rev. 73:407-450.
37. Hannan TJ, Mysorekar IU, Hung CS, Isaacson-Schmid ML, Hultgren SJ. 2010. Early severe inflammatory responses to uropathogenic E. coli predispose to chronic and recurrent urinary tract infection. PLoS Pathog. 6:e1001042. doi:10.1371/journal.ppat.1001042.
38. Duell BL, Carey AJ, Tan CK, Cui X, Webb RI, Totsika M, Schembri MA, Derrington P, Irving-Rodgers H, Brooks AJ, Cripps AW, Crowley M, Ulett GC. 2012. Innate transcriptional networks activated in bladder in response to uropathogenic Escherichia coli drive diverse biological pathways and rapid synthesis of IL-10 for defense against bacterial urinary tract infection. J. Immunol. 188:781-792.
39. Tan CK, Carey AJ, Cui X, Webb RI, Ipe D, Crowley M, Cripps AW, Benjamin WH, Jr, Ulett KB, Schembri MA, Ulett GC. 2012. Genomewide mapping of cystitis due to Streptococcus agalactiae and Escherichia coli in mice identifies a unique bladder transcriptome that signifies pathogenspecific antimicrobial defense against urinary tract infection. Infect. Immun. 80:3145-3160.
40. Sathiananthamoorthy S, Swamy S, Kupelian A, Horsley H, Gill K, Collins L, Malone-Lee J. 2012. "Mixed growth of doubtful significance" is extremely significant in patients with lower urinary tract symptoms. Neurourol. Urodyn. 31:736-737.
41. Bartlett RC, Treiber N. 1984. Clinical significance of mixed bacterial cultures of urine. Am. J. Clin. Pathol. 82:319-322.
42. Hunstad DA, Justice SS. 2010. Intracellular lifestyles and immune evasion strategies of uropathogenic Escherichia coli. Annu. Rev. Microbiol. 64:203-221.
43. Hoiby N, Bjarnsholt T, Givskov M, Molin S, Ciofu O. 2010. Antibiotic resistance of bacterial biofilms. Int. J. Antimicrob. Agents 35:322-332.