Quantitation of cytomegalovirus DNA load in dried blood spots correlates well with plasma viral load

Journal of Clinical Microbiology

Volumn 51, Issue 7, 2013, Pages 2360-2364

  Limaye, Ajit P ^a   Hayes, Tracy K Santo ^a   Huang, Meei Li ^a   Magaret, Amalia ^a,b   Boeckh, Michael ^a,b   Jerome, Keith R ^a,b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b Fred Hutchinson Cancer Research Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GANCICLOVIR; VIRUS DNA;

ADULT; AGED; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; DRIED BLOOD SPOT TESTING; FEMALE; HUMAN; LIMIT OF DETECTION; MALE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROSPECTIVE STUDY; QUANTITATIVE ANALYSIS; VALIDATION PROCESS; VEIN PUNCTURE; VIRUS DETECTION; VIRUS LOAD; BLOOD; COMPARATIVE STUDY; DESICCATION; EVALUATION STUDY; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; MIDDLE AGED; PLASMA; PROCEDURES; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TRANSPLANTATION; VIROLOGY;

CYTOMEGALOVIRUS;

ADULT; AGED; BLOOD; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DESICCATION; DNA, VIRAL; FEMALE; HUMANS; MALE; MIDDLE AGED; PLASMA; PROSPECTIVE STUDIES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; TRANSPLANTATION; VIRAL LOAD;

EID: 84879437833     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00316-13     Document Type: Article

References (28)

1. Boeckh M, Boivin G. 1998. Quantitation of cytomegalovirus: methodologic aspects and clinical applications. Clin. Microbiol. Rev. 11:533-554.
2. Barbi M, Binda S, Caroppo S, Calvario A, Germinario C, Bozzi A, Tanzi ML, Veronesi L, Mura I, Piana A, Solinas G, Pugni L, Bevilaqua G, Mosca F. 2006. Multicity Italian study of congenital cytomegalovirus infection. Pediatr. Infect. Dis. J. 25:156-159.
3. Barbi M, MacKay WG, Binda S, van Loon AM. 2008. External quality assessment of cytomegalovirus DNA detection on dried blood spots. BMC Microbiol. 8:2. doi:10.1186/1471-2180-8-2.
4. de Vries JJ, Vesseur A, Rotteveel LJ, Korver AM, Rusman LG, Wessels E, Kroes AC, Mylanus EA, Oudesluys-Murphy AM, Frijns JH, Vossen AC. 2013. Cytomegalovirus DNA detection in dried blood spots and perilymphatic fluids from pediatric and adult cochlear implant recipients with prelingual deafness. J. Clin. Virol. 56:113-117.
5. Johansson PJ, Jonsson M, Ahlfors K, Ivarsson SA, Svanberg L, Guthenberg C. 1997. Retrospective diagnostics of congenital cytomegalovirus infection performed by polymerase chain reaction in blood stored on filter paper. Scand. J. Infect. Dis. 29:465-468.
6. Scanga L, Chaing S, Powell C, Aylsworth AS, Harrell LJ, Henshaw NG, Civalier CJ, Thorne LB, Weck K, Booker J, Gulley ML. 2006. Diagnosis of human congenital cytomegalovirus infection by amplification of viral DNA from dried blood spots on perinatal cards. J. Mol. Diagn. 8:240-245.
7. Shibata M, Takano H, Hironaka T, Hirai K. 1994. Detection of human cytomegalovirus DNA in dried newborn blood filter paper. J. Virol. Methods 46:279-285.
8. Soetens O, Vauloup-Fellous C, Foulon I, Dubreuil P, De Saeger B, Grangeot-Keros L, Naessens A. 2008. Evaluation of different cytomegalovirus (CMV) DNA PCR protocols for analysis of dried blood spots from consecutive cases of neonates with congenital CMV infections. J. Clin. Microbiol. 46:943-946.
9. Vauloup-Fellous C, Ducroux A, Couloigner V, Marlin S, Picone O, Galimand J, Loundon N, Denoyelle F, Grangeot-Keros L, Leruez-Ville M. 2007. Evaluation of cytomegalovirus (CMV) DNA quantification in dried blood spots: retrospective study of CMV congenital infection. J. Clin. Microbiol. 45:3804-3806.
10. Walter S, Atkinson C, Sharland M, Rice P, Raglan E, Emery VC, Griffiths PD. 2008. Congenital cytomegalovirus: association between dried blood spot viral load and hearing loss. Arch. Dis. Child. 93:F280-F285.
11. Yamagishi Y, Miyagawa H, Wada K, Matsumoto S, Arahori H, Tamura A, Taniguchi H, Kanekiyo T, Sashihara J, Yoda T, Kitagawa M, Ozono K. 2006. CMV DNA detection in dried blood spots for diagnosing congenital CMV infection in Japan. J. Med. Virol. 78:923-925.
12. Yamamoto AY, Mussi-Pinhata MM, Pinto PC, Figueiredo LT, Jorge SM. 2001. Usefulness of blood and urine samples collected on filter paper in detecting cytomegalovirus by the polymerase chain reaction technique. J. Virol. Methods 97:159-164.
13. Nesbitt SE, Cook L, Jerome KR. 2004. Cytomegalovirus quantitation by real-time PCR is unaffected by delayed separation of plasma from whole blood. J. Clin. Microbiol. 42:1296-1297.
14. Cassol S, Salas T, Gill MJ, Montpetit M, Rudnik J, Sy CT, O'Shaughnessy MV. 1992. Stability of dried blood spot specimens for detection of human immunodeficiency virus DNA by polymerase chain reaction. J. Clin. Microbiol. 30:3039-3042.
15. Chaisomchit S, Wichajarn R, Janejai N, Chareonsiriwatana W. 2005. Stability of genomic DNA in dried blood spots stored on filter paper. Southeast Asian J. Trop. Med. Public Health 36:270-273.
16. Leelawiwat W, Young NL, Chaowanachan T, Ou CY, Culnane M, Vanprapa N, Waranawat N, Wasinrapee P, Mock PA, Tappero J, McNicholl JM. 2009. Dried blood spots for the diagnosis and quantitation of HIV-1: stability studies and evaluation of sensitivity and specificity for the diagnosis of infant HIV-1 infection in Thailand. J. Virol. Methods 155:109-117.
17. Cassol S, Weniger BG, Babu PG, Salminen MO, Zheng X, Htoon MT, Delaney A, O'Shaughnessy M, Ou CY. 1996. Detection of HIV type 1 env subtypes A, B, C, and E in Asia using dried blood spots: a new surveillance tool for molecular epidemiology. AIDS Res. Hum. Retroviruses 12:1435-1441.
18. Boeckh M, Huang M, Ferrenberg J, Stevens-Ayers T, Stensland L, Nichols WG, Corey L. 2004. Optimization of quantitative detection of cytomegalovirus DNA in plasma by real-time PCR. J. Clin. Microbiol. 42:1142-1148.
19. Agresti A. 2007. An introduction to categorical data analysis, 2nd ed. Wiley, Edison, NJ.
20. Hoogtanders K, van der Heijden J, Christiaans M, van de Plas A, van Hooff J, Stolk L. 2007. Dried blood spot measurement of tacrolimus is promising for patient monitoring. Transplantation 83:237-238.
21. Kunzle N, Petignat C, Francioli P, Vogel G, Seydoux C, Corpataux JM, Sahli R, Meylan PR. 2000. Preemptive treatment approach to cytomegalovirus (CMV) infection in solid organ transplant patients: relationship between compliance with the guidelines and prevention of CMV morbidity. Transpl. Infect. Dis. 2:118-126.
22. Kusne S, Grossi P, Irish W, St George K, Rinaldo C, Rakela J, Fung J. 1999. Cytomegalovirus PP65 antigenemia monitoring as a guide for preemptive therapy: a cost effective strategy for prevention of cytomegalovirus disease in adult liver transplant recipients. Transplantation 68:1125-1131.
23. Caliendo AM, Schuurman R, Yen-Lieberman B, Spector SA, Andersen J, Manjiry R, Crumpacker C, Lurain NS, Erice A. 2001. Comparison of quantitative and qualitative PCR assays for cytomegalovirus DNA in plasma. J. Clin. Microbiol. 39:1334-1338.
24. Waggoner J, Ho DY, Libiran P, Pinsky BA. 2012. Clinical significance of low cytomegalovirus DNA levels in human plasma. J. Clin. Microbiol. 50:2378-2383.
25. Humar A, Gregson D, Caliendo AM, McGeer A, Malkan G, Krajden M, Corey P, Greig P, Walmsley S, Levy G, Mazzulli T. 1999. Clinical utility of quantitative cytomegalovirus viral load determination for predicting cytomegalovirus disease in liver transplant recipients. Transplantation 68: 1305-1311.
26. Pollack M, Heugel J, Xie H, Leisenring W, Storek J, Young JA, Kukreja M, Gress R, Tomblyn M, Boeckh M. 2011. An international comparison of current strategies to prevent herpesvirus and fungal infections in hematopoietic cell transplant recipients. Biol. Blood Marrow Transplant. 17: 664-673. doi:10.1016/j.bbmt.2010.07.026.
27. Caliendo AM. 2013. Editorial commentary: the long road toward standardization of viral load testing for cytomegalovirus. Clin. Infect. Dis. 56:374-375. doi:10.1093/cid/cis905.
28. Kraft CS, Armstrong WS, Caliendo AM. 2012. Interpreting quantitative cytomegalovirus DNA testing: understanding the laboratory perspective. Clin. Infect. Dis. 54:1793-1797.