High throughput imaging to the diatom Cyclotella cryptica demonstrates substantial cell-to-cell variability in the rate and extent of triacylglycerol accumulation

Algal Research

Volumn 2, Issue 3, 2013, Pages 244-252

  Traller, Jesse C ^a   Hildebrand, Mark ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

BODIPY; Cell to cell variability; Diatom; Imaging flow cytometry; Nutrient starvation; Triacylglycerol

Indexed keywords

BACILLARIOPHYTA; CYCLOTELLA CRYPTICA;

EID: 84879273320     PISSN: 22119264     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.algal.2013.03.003     Document Type: Article

References (46)

1. Chisti Y. Biodiesel from microalgae. Biotechnology Advances 2007, 25:294-306.
2. Chisti Y. Biodiesel from microalgae beats bioethanol. Trends in Biotechnology 2008, 26:1-6.
3. Sheehan J., Dunahay T., Benemann J., Roessler P. A look back at the US department of energy's aquatic species program: biodiesel from algae 1998.
4. Shifrin N., Chisholm S. Phytoplankton lipids: interspecific differences and effects of nitrate, silicate and light-dark cycles. Journal of Phycology 1981, 17:374-384.
5. Pienkos P., Darzins A. The promise and challenges of microalgal-derived biofuels. Biofuels 2009, 3:431-440.
6. Hildebrand M., Davis A.K., Smith S.R., Traller J.C., Abbriano R. The place of diatoms in the biofuels industry. Biofuels 2012, 3:221-240.
7. Werner D. Die Kieselsäure im Stoffwechsel von Cyclotella cryptica Reimann, Lewin und Guillard. Archives of Microbiology 1966, 55:278-308.
8. Roessler P. Effects of silicon deficiency on lipid composition and metabolism in the diatom Cyclotella cryptica. Journal of Phycology 1988, 24:394-400.
9. Huesemann M., Hausmann T., Bartha R. Biomass productivities in wild type and pigment mutant of Cyclotella sp. (diatom). Applied Biochemistry 2009, 3:507-526.
10. Kröger N., Bergsdorf C., Sumper M. A new calcium binding glycoprotein family constitutes a major diatom cell wall component. The EMBO Journal 1994, 3:4676-4683.
11. Kröger N., Sumper M. Diatom cell wall proteins and the cell biology of silica biomineralization. Protist 1998, 149:213-219.
12. Hildebrand M. Diatoms, biomineralization processes, and genomics. Chemical Reviews 2008, 108:4855-4874.
13. Martin-Jézéquel V., Hildebrand M. Silicon metabolism in diatoms: implications for growth. Journal of Phycology 2000, 36:821-840.
14. Brzezinski M. The Si:C:N ratio of marine diatoms: interspecific variability and the effect of some environmental variables. Journal of Phycology 1985, 21:347-357.
15. Yu E., Zendejas F., Lane P., Gaucher S. Triacylglycerol accumulation and profiling in the model diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum (Baccilariophyceae) during starvation. Journal of Applied Phycology 2009, 21:669-681.
16. Claquin P., Martin-Jézéquel V. Uncoupling of silicon compared with carbon and nitrogen metabolisms and the role of the cell cycle in continuous cultures of Thalassiosira pseudonana (Bacillariophyceae) under light, nitrogen, and phosphorous control. Journal of Phycology 2002, 38:922-930.
17. Hu Q., Sommerfeld M., Jarvis E., Ghirardi M. Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. The Plant Journal 2008, 54:621-639.
18. Guarnieri M.T., Nag A., Smolinski S.L., al Darzins, Seibert M., Pienkos P.T. Examination of triacylglycerol biosynthetic pathways via de novo transcriptomic and proteomic analyses in an unsequenced microalga. PLoS One 2011, 6:e25851.
19. Jamers A., Blust R., Coen W. Omics in algae: paving the way for a systems biological understanding of algal stress phenomena?. Aquatic Toxicology 2009, 92:114-121.
20. Hockin N., Mock T., Mulholland F., Kopriva S. The response of diatom central carbon metabolism to nitrogen starvation is different from that of green algae and higher plants. Plant Physiology 2012, 158:299-312.
21. Smith S., Abbriano R., Hildebrand M. Comparative analysis of diatom genomes reveals substantial differences in the organization of carbon partitioning pathways. Algal Research 2012, 1:2-16.
22. Cooksey K., Guckert J., Williams S. Fluorometric determination of the neutral lipid content of microalgal cells using Nile Red. Journal of Microbiological Methods 1987, 6:333-345.
23. Chen W., Zhang C., Song L., Sommerfeld M. A high throughput Nile Red method for quantitative measurement of neutral lipids in microalgae. Journal of Microbiological Methods 2009, 77:41-47.
24. Govender T., Ramanna L., Rawat I., Bux F. BODIPY staining, an alternative to the Nile Red fluorescence method for the evaluation of intracellular lipids in microalgae. Bioresource Technology 2012, 114:507-511.
25. Cooper M.S., Hardin W.R., Petersen T.W., Cattolico R.A. Visualizing "green oil" in live algal cells. Journal of Bioscience and Bioengineering 2010, 109:198-201.
26. Bertozzini E., Galluzzi L., Penna A., Magnani M. Application of the standard addition method for the absolute quantification of neutral lipids in microalgae using Nile red. Journal of Microbiological 2011, 17-23.
27. Guzmán H., Valido A., Presmanes K., Duarte L. Quick estimation of intraspecific variation of fatty acid composition in Dunaliella salina using flow cytometry and Nile Red. Journal of Applied Phycology 2012, 24:1237-1243.
28. Xu D., Gao Z., Li F., Fan X., Zhang X., Ye N., et al. Detection and quantitation of lipid in the microalga Tetraselmis subcordiformis (Wille) Butcher with BODIPY 505/515 staining. Bioresource Technology 2013, 127:386-390.
29. Toriello N., Douglas E., Thaitrong N. Integrated microfluidic bioprocessor for single-cell gene expression analysis. Proceedings of the National Academy of Sciences 2008, 105:20173-20178.
30. Wang D., Bodovitz S. Single cell analysis: the new frontier in "omics". Trends in Biotechnology 2010, 28:281-290.
31. Stewart E.J., Madden R., Paul G., Taddei F. Aging and death in an organism that reproduces by morphologically symmetric division. PLoS Biology 2005, 3:e45.
32. Voronova E., Il'ash L., Pogosyan S., Ulanova A., Matorin D., Cho M., Rubin A. Intrapopulation heterogeneity of the fluorescence parameters of the marine plankton alga Thalassiosira weissflogii at various nitrogen levels. Microbiology 2009, 78:469-478.
33. Darley W.M., Volcani B.E. Role of silicon in diatom metabolism. Experimental Cell Research 1969, 58:334-342.
34. Beum P., Lindorfer M., Hall B., George T. Quantitative analysis of protein co-localization on B cells opsonized with rituximab and complement using the ImageStream multispectral imaging flow cytometer. Journal of Immunological Methods 2006, 317:90-99.
35. Brzezinski M., Olson R., Chisholm S. Silicon availability and cell-cycle progression in marine diatoms. Marine Ecology Progress Series 1990, 67:83-96.
36. Healey F., Coombs J., Volcani B. Changes in pigment content of the diatom Navicula pelliculosa (Bréb.) Hilse in silicon-starvation synchrony. Archives of Microbiology 1967, 59:131-142.
37. 505/515 lipid fluorescence method for applications in biofuel-directed microalgae production. Journal of Microbiological Methods 2012, 90:137-143.
38. Laney S., Olson R., Sosik H. Diatoms favor their younger daughters. Limnology and Oceanography 2012, 57:1572-1578.
39. Maumus F., Rabinowicz P., Bowler C. Stemming epigenetics in marine stramenopiles. Current Genomics 2011, 12:357-370.
40. Moellering E., Benning C. RNA interference silencing of a major lipid droplet protein affects lipid droplet size in Chlamydomonas reinhardtii. Eukaryotic Cell 2010, 9:97-106.
41. Goodson C., Roth R., Wang Z.T., Goodenough U. Structural correlates of cytoplasmic and chloroplast lipid body synthesis in Chlamydomonas reinhardtii and stimulation of lipid body production with acetate boost. Eukaryotic Cell 2011, 10:1592-1606.
42. Siaut M., Cuiné S., Cagnon C., Fessler B. Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves. BMC Biotechnology 2011, 11:1-15.
43. Wang Z., Ullrich N., Joo S., Waffenschmidt S. Algal lipid bodies: stress induction, purification, and biochemical characterization in wild-type and starchless Chlamydomonas reinhardtii. Eukaryotic Cell 2009, 8:1856-1868.
44. Vaulot D., Partensky F. Cell cycle distributions of prochlorophytes in the North Western Mediterranean Sea. Deep Sea Research Part A Oceanographic Research Papers 1992, 39:727-742.
45. Kwok A., Wong J. Lipid biosynthesis and its coordination with cell cycle progression. Plant and Cell Physiology 2005, 46:1973-1986.
46. Peano C., Severgnini M., Cifola I. Transcriptome amplification methods in gene expression profiling, Expert Review Of Molecular Archives. Neoplasia 2006, 8:153-162.