Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes

Advanced Healthcare Materials

Volumn 2, Issue 11, 2013, Pages 1440-1444

  Frampton, John P ^a   Shi, Huilin ^b   Kao, Albert ^a   Parent, Jack M ^b   Takayama, Shuichi ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Aqueous two phase system; Cardiomyocyte; Dextran; Polyethylene glycol; Stem cell

Indexed keywords

CELL CULTURE; DEXTRAN; POLYETHYLENE GLYCOLS; POLYETHYLENES; SOLVENT EXTRACTION;

AQUEOUS TWO PHASE SYSTEM; CARDIOMYOCYTES; FEEDER CELLS; GLASS CAPILLARIES; INDUCED PLURIPOTENT STEM CELLS; MOUSE EMBRYONIC FIBROBLASTS; PNEUMATIC PUMPS; SUBCELLULAR RESOLUTION;

STEM CELLS;

ALPHA ACTININ; DEXTRAN; PROTEINASE; TROPONIN T; TRYPSIN;

AQUEOUS TWO PHASE SYSTEM; ARTICLE; CELL ADHESION; CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL POPULATION; CELL VIABILITY; CHROMOSOME ANALYSIS; COCULTURE; DRUG DELIVERY SYSTEM; EMBRYOID BODY; FEEDER CELL; GENE DELIVERY SYSTEM; HEART MUSCLE CELL; HUMAN; HUMAN CELL; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; STEM CELL;

AQUEOUS TWO-PHASE SYSTEM; CARDIOMYOCYTE; DEXTRAN; POLYETHYLENE GLYCOL; STEM CELL;

ANIMALS; CELL DIFFERENTIATION; CELL SEPARATION; COCULTURE TECHNIQUES; DEXTRANS; FEEDER CELLS; HEK293 CELLS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MICE; MYOCYTES, CARDIAC; PEPTIDE HYDROLASES; POLYETHYLENE GLYCOLS; WATER;

EID: 84879044825     PISSN: 21922640     EISSN: 21922659     Source Type: Journal    
DOI: 10.1002/adhm.201300049     Document Type: Article

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