Radio-metabolite analysis of carbon-11 biochemical partitioning to non-structural carbohydrates for integrated metabolism and transport studies

Plant and Cell Physiology

Volumn 54, Issue 6, 2013, Pages 1016-1025

  Babst, Benjamin A ^a   Karve, Abhijit A ^a   Judt, Tatjana ^b  

^a BROOKHAVEN NATIONAL LABORATORY   (United States)

^b JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Carbohydrate metabolism; Carbon 11; Leaf export; Phloem transport; Starch; Sugars

Indexed keywords

CARBOHYDRATE; CARBON; STARCH;

ARTICLE; CARBOHYDRATE METABOLISM; CARBON-11; CHEMISTRY; LEAF EXPORT; METABOLISM; PHLOEM; PHLOEM TRANSPORT; PLANT; PLANT LEAF; THIN LAYER CHROMATOGRAPHY; TRANSPORT AT THE CELLULAR LEVEL;

CARBOHYDRATE METABOLISM; CARBON-11; LEAF EXPORT; PHLOEM TRANSPORT; STARCH; SUGARS;

BIOLOGICAL TRANSPORT; CARBOHYDRATE METABOLISM; CARBOHYDRATES; CARBON RADIOISOTOPES; CHROMATOGRAPHY, THIN LAYER; PHLOEM; PLANT LEAVES; PLANTS; STARCH;

EID: 84878841018     PISSN: 00320781     EISSN: 14719053     Source Type: Journal    
DOI: 10.1093/pcp/pct045     Document Type: Article

References (29)

1. Ainsworth, E.A. and Bush, D.R. (2011) Carbohydrate export from the leaf: a highly regulated process and target to enhance photosynthesis and productivity. Plant Physiol. 155: 64-69.
2. Babst, B.A., Ferrieri, R.A., Gray, D.W., Lerdau, M., Schlyer, D.J., Schueller, M.J. et al. (2005) Jasmonic acid induces rapid changes in carbon transport and partitioning in Populus. New Phytol. 167: 63-72.
3. Cross, J.M., Von Korff, M., Altmann, T., Bartzetko, L., Sulpice, R., Gibon, Y. et al. (2006) Variation of enzyme activities and metabolite levels in 24 Arabidopsis accessions growing in carbon-limited conditions. Plant Physiol. 142: 1574-1588.
4. Ferrieri, A.P., Agtuca, B., Appel, H.M., Ferrieri, R.A. and Schultz, J.C. (2013) Temporal changes in allocation and partitioning of new carbon as 11C elicited by simulated herbivory suggest that roots shape aboveground responses in Arabidopsis thaliana. Plant Physiol. 161: 692-704.
5. Ferrieri, R.A., Gray, D.W., Babst, B.A., Schueller, M.J., Schlyer, D.J., Thorpe, M.R. et al. (2005) Use of carbon-11 in Populus shows that exogenous jasmonic acid increases biosynthesis of isoprene from recently fixed carbon. Plant Cell Environ. 28: 591-602.
6. Ferrieri, R.A. and Wolf, A.P. (1983) The chemistry of positron emitting nucleogenic atoms with regard to preparation of labeled compounds of practical utility. Radiochim. Acta 34: 69-83.
7. Fischer, B.U., Frehner, M., Hebeisen, T., Zanetti, S., Stadelmann, F., Lüscher, A. et al. (1997) Source-sink relations in Lolium perenne L. as reflected by carbohydrate concentrations in leaves and pseudo-stems during regrowth in a free air carbon dioxide enrichment (FACE) experiment. Plant Cell Environ. 20: 945-953.
8. Gibon, Y., Pyl, E.-T., Sulpice, R., Lunn, J.E., Hohne, M., Gunther, M. et al. (2009) Adjustment of growth, starch turnover, protein content and central metabolism to a decrease of the carbon supply when Arabidopsis is grown in very short photoperiods. Plant Cell Environ. 32: 859-874.
9. Gomez, S., Ferrieri, R.A., Schueller, M.J. and Orians, C.M. (2010) Methyl jasmonate elicits rapid changes in carbon and nitrogen dynamics in tomato. New Phytol. 188: 835-844.
10. Gould, N., Thorpe, M.R., Pritchard, J., Christeller, J.T., Williams, L.E., Roeb, G. et al. (2012) AtSUC2 has a role for sucrose retrieval along the phloem pathway: evidence from carbon-11 tracer studies. Plant Sci. 188-189: 97-101.
11. Graf, A., Schlereth, A., Stitt, M. and Smith, A.M. (2010) Circadian control of carbohydrate availability for growth in Arabidopsis plants at night. Proc. Natl Acad. Sci. USA 107: 9458-9463.
12. Hanik, N., Gomez, S., Best, M., Schueller, M.J., Orians, C.M. and Ferrieri, R.A. (2010) Partitioning of new carbon as 11C in Nicotiana tabacum reveals new insight into methyl jasmonate induced changes in metabolism. J. Chem. Ecol. 36: 1058-1067.
13. Jahnke, S., Menzel, M.I., van Dusschoten, D., Roeb, G.W., Buhler, J., Minwuyelet, S. et al. (2009) Combined MRI-PET dissects dynamic changes in plant structures and functions. Plant J. 59: 634-644.
14. Jensen, K.H., Liesche, J., Bohr, T. and Schulz, A. (2012) Universality of phloem transport in seed plants. Plant Cell Environ. 35: 1065-1076.
15. Kawachi, N., Kikuchi, K., Suzui, N., Ishii, S., Fujimaki, S., Ishioka, N.S. et al. (2011a) Imaging of carbon translocation to fruit using carbon-11-labeled carbon dioxide and positron emission tomography. IEEE Trans. Nucl. Sci. 58: 395-399.
16. Kawachi, N., Suzui, N., Ishii, S., Ito, S., Ishioka, N.S., Yamazaki, H. et al. (2011b) Real-time whole-plant imaging of 11C translocation using positron-emitting tracer imaging system. Nucl. Instrum. Methods Phys. Res. A 648: S317-S320.
17. Klaus, R., Fischer, W. and Hauck, H.E. (1989) Use of a new adsorbent in the separation and detection of glucose and fructose by HPTLC. Chromatographia 28: 364-366.
18. Klaus, R., Fischer, W. and Hauck, H.E. (1990) Application of a thermal in situ reaction for fluorimetric detection of carbohydrates on NH2-layers. Chromatographia 29: 467-472.
19. Komolprasert, V. and Ofoli, R.Y. (1991) Starch hydrolysis kinetics of Bacillus licheniformis alpha-amylase. J. Chem. Technol. Biotechnol. 51: 209-223.
20. Lunn, J.E., Feil, R., Hendriks, J.H.M., Gibon, Y., Morcuende, R., Osuna, D. et al. (2006) Sugar-induced increases in trehalose 6-phosphate are correlated with redox activation of ADPglucose pyrophosphorylase and higher rates of starch synthesis in Arabidopsis thaliana. Biochem. J. 397: 139-148.
21. Minchin, P.E.H. and Thorpe, M.R. (2003) Using the short-lived isotope 11C in mechanistic studies of photosynthate transport. Funct. Plant Biol. 30: 83-841.
22. Payyavula, R., Tay, K.H.C., Tsai, C.-J. and Harding, S.A. (2011) The sucrose transporter family in Populus: the importance of a tonoplast PtsSUT4 to biomass and carbon partitioning. Plant J. 65: 757-770.
23. Schwachtje, J., Minchin, P.E.H., Jahnke, S., van Dongen, J.T., Schittko, U. and Baldwin, I.T. (2006) SNF1-related kinases allow plants to tolerate herbivory by allocating carbon to roots. Proc. Natl Acad. Sci. USA 103: 12935-12940.
24. Schwender, J. (2008) Metabolic flux analysis as a tool in metabolic engineering of plants. Curr. Opin. Biotechnol. 19: 131-137.
25. Sherma, J. and Zulick, D.L. (1996) Determination of fructose, glucose, and sucrose in beverages by high-performance thin layer chromatography. Acta Chromatogr. 6: 7-13.
26. Slewinski, T.L., Meeley, R. and Braun, D.M. (2009) Sucrose transporter1 functions in phloem loading in maize leaves. J. Exp. Bot. 60: 881-892.
27. Smith, A.M. and Stitt, M. (2007) Coordination of carbon supply and plant growth. Plant Cell Environ.t 30: 1126-1149.
28. Sulpice, R., Pyl, E.-T., Ishihara, H., Trenkamp, S., Steinfath, M., Witucka-Wall, H. et al. (2009) Starch as a major integrator in the regulation of plant growth. Proc. Natl Acad. Sci. USA 106: 10348-10353.
29. Turgeon, R. (2010) The role of phloem loading reconsidered. Plant Physiol. 152: 1817-1823.