Engineering of an endogenous phytoene desaturase gene as a dominant selectable marker for Chlamydomonas reinhardtii transformation and enhanced biosynthesis of carotenoids

Process Biochemistry

Volumn 48, Issue 5-6, 2013, Pages 788-795

  Liu, Jin ^a,d   Gerken, Henri ^b   Huang, Junchao ^c   Chen, Feng ^d  

^a UNIVERSITY OF MARYLAND CENTER FOR ENVIRONMENTAL SCIENCE   (United States)

^b ARIZONA STATE UNIVERSITY   (United States)

^c KUNMING INSTITUTE OF BOTANY   (China)

^d PEKING UNIVERSITY   (China)

Author keywords

Carotenoids; Chlamydomonas reinhardtii; Norflurazon; Phytoene desaturase; Selectable marker; Site directed mutation

Indexed keywords

CAROTENOIDS; CHLAMYDOMONAS REINHARDTII; NORFLURAZON; PHYTOENE DESATURASE; SELECTABLE MARKERS; SITE-DIRECTED MUTATION;

ALGAE; AMINO ACIDS; BIOCHEMISTRY; CLONING; GENES; HERBICIDES;

PIGMENTS;

CHLAMYDOMONAS REINHARDTII; CHLOROPHYTA;

EID: 84878681236     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2013.04.020     Document Type: Article

References (31)

1. Q. Hu, M. Sommerfeld, E. Jarvis, M. Ghirardi, M. Posewitz, and M. Seibert Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances Plant J 54 2008 621 639
2. Y. Li, D. Han, G. Hu, D. Dauvillee, M. Sommerfeld, and S. Ball Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol Metab Eng 12 2010 387 391
3. X. Li, E.R. Moellering, B. Liu, C. Johnny, M. Fedewa, and B.B. Sears A galactoglycerolipid lipase is required for triacylglycerol accumulation and survival following nitrogen deprivation in Chlamydomonas reinhardtii Plant Cell 2012 10.1105/tpc.1112.105106 [in press]
4. K. Yoon, D. Han, Y. Li, M. Sommerfeld, and Q. Hu Phospholipid: diacylglycerol acyltransferase is a multifunctional enzyme involved in membrane lipid turnover and degradation while synthesizing triacylglycerol in the unicellular green microalga Chlamydomonas reinhardtii Plant Cell 24 2012 3708 3724
5. D. Stevens, S. Purton, and J. Rochaix The bacterial phleomycin resistance gene ble as a dominant selectable marker in Chlamydomonas Mol Gen Genet MGG 251 1996 23 30
6. V. Lumbreras, D.R. Stevens, and S. Purton Efficient foreign gene expression in Chlamydomonas reinhardtii mediated by an endogenous intron Plant J 14 1998 441 447
7. I.A. Sizova, T.V. Lapina, O.N. Frolova, N.N. Alexandrova, K.E. Akopiants, and V.N. Danilenko Stable nuclear transformation of Chlamydomonas reinhardtii with a Streptomyces rimosus gene as the selective marker Gene 181 1996 13 18
8. M. Vila, E. Díaz-Santos, M. de la Vega, H. Rodríguez, Á. Vargas, and R. León Promoter trapping in microalgae using the antibiotic paromomycin as selective agent Mar Drugs 10 2012 2749 2765
9. H. Cerutti, A.M. Johnson, N.W. Gillham, and J.E. Boynton Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas Plant Cell 9 1997 925 945
10. J. Steinbrenner, and G. Sandmann Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis Appl Environ Microb 72 2006 7477 7484
11. K.L. Kindle High-frequency nuclear transformation of Chlamydomonas reinhardtii Proc Natl Acad Sci USA 87 1990 1228 1232
12. C. Schönfeld, L. Wobbe, R. Borgstädt, A. Kienast, P.J. Nixon, and O. Kruse The nucleus-encoded protein MOC1 is essential for mitochondrial light acclimation in Chlamydomonas reinhardtii J Biol Chem 279 2004 50366 50374
13. I. Couso, B.F. Cordero, M.Á. Vargas, and H. Rodríguez Efficient heterologous transformation of Chlamydomonas reinhardtii npq2 mutant with the zeaxanthin epoxidase gene isolated and characterized from Chlorella zofingiensis Mar Drugs 10 2012 1955 1976
14. D. Chamovitz, I. Pecker, and J. Hirschberg The molecular-basis of resistance to the herbicide norflurazon Plant Mol Biol 16 1991 967 974
15. J. Liu, Y. Zhong, Z. Sun, J. Huang, G. Sandmann, and F. Chen One amino acid substitution in phytoene desaturase makes Chlorella zofingiensis resistant to norflurazon and enhances the biosynthesis of astaxanthin Planta 232 2010 61 67
16. D. Chamovitz, G. Sandmann, and J. Hirschberg Molecular and biochemical-characterization of herbicide-resistant mutants of cyanobacteria reveals that phytoene desaturation is a rate-limiting step in carotenoid biosynthesis J Biol Chem 268 1993 17348 17353
17. P.T. Tran, M.N. Sharifi, S. Poddar, R.M. Dent, and K.K. Niyogi Intragenic enhancers and suppressors of phytoene desaturase mutations in Chlamydomonas reinhardtii PLoS One 7 2012 e42196
18. C.N. Stewart, and L.E. Via A rapid CTAB DNA isolation technique useful for rapid fingerprinting and other PCR applications Biotechniques 14 1993 748 751
19. N. Misawa, S. Kajiwara, K. Kondo, A. Yokoyama, Y. Satomi, and T. Saito Canthaxanthin biosynthesis by the conversion of methylene to keto groups in a hydrocarbon beta-carotene by a single gene Biochem Biophys Res Commun 209 1995 867 876
20. J. Breitenbach, C.F. Zhu, and G. Sandmann Bleaching herbicide norflurazon inhibits phytoene desaturase by competition with the cofactors J Agric Food Chem 49 2001 5270 5272
21. J. Liu, Z. Sun, Y. Zhong, H. Gerken, J. Huang, and F. Chen Utilization of cane molasses towards cost-saving astaxanthin production by a Chlorella zofingiensis mutant J Appl Phycol 2013 10.1007/s10811-013-9974-x [in press]
22. -Ct method Methods 25 2001 402 408
23. K. Tamura, J. Dudley, M. Nei, and S.M.E.G.A4. Kumar Molecular evolutionary genetics analysis (MEGA) software version 4.0 Mol Biol Evol 24 2007 1596 1599
24. G. Sandmann, H. Linden, and P. Boger Enzyme-kinetic studies on the interaction of norflurazon with phytoene desaturase Z Naturforsch C 44 1989 787 790
25. I. Couso, M. Vila, J. Vigara, and B.F. Cordero Angeles Vargas M, Rodriguez H, Leon R. Synthesis of carotenoids and regulation of the carotenoid biosynthesis pathway in response to high light stress in the unicellular microalga Chlamydomonas reinhardtii Eur J Phycol 47 2012 223 232
26. M. Lohr, C-S. Im, and A.R. Grossman Genome-based examination of chlorophyll and carotenoid biosynthesis in Chlamydomonas reinhardtii Plant Physiol 138 2005 490 515
27. B. Cordero, I. Couso, R. León, H. Rodríguez, and M. Vargas Enhancement of carotenoids biosynthesis in Chlamydomonas reinhardtii by nuclear transformation using a phytoene synthase gene isolated from Chlorella zofingiensis Appl Microbiol Biotechnol 91 2011 341 351
28. I. Couso, M. Vila, H. Rodriguez, M.A. Vargas, and R. León Overexpression of an exogenous phytoene synthase gene in the unicellular alga Chlamydomonas reinhardtii leads to an increase in the content of carotenoids Biotechnol Progr 27 2011 54 60
29. N. Misawa, K. Masamoto, T. Hori, T. Ohtani, P. Boger, and G. Sandmann Expression of an Erwinia phytoene desaturase gene not only confers multiple resistance to herbicides interfering with carotenoid biosynthesis but also alters xanthophyll metabolism in transgenic plants Plant J 6 1994 481 489
30. S. Romer, P.D. Fraser, J.W. Kiano, C.A. Shipton, N. Misawa, and W. Schuch Elevation of the provitamin A content of transgenic tomato plants Nat Biotechnol 18 2000 666 669
31. M. Vila, I. Couso, and R. León Carotenoid content in mutants of the chlorophyte Chlamydomonas reinhardtii with low expression levels of phytoene desaturase Process Biochem 43 2008 1147 1152