Challenges of Culturing Human Norovirus in Three-Dimensional Organoid Intestinal Cell Culture Models

PLoS ONE

Volumn 8, Issue 6, 2013, Pages

  Papafragkou, Efstathia ^a,b   Hewitt, Joanne ^c   Park, Geun Woo ^a   Greening, Gail ^c   Vinjé, Jan ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^c INSTITUTE OF ENVIRONMENTAL SCIENCE AND RESEARCH   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

BETA CATENIN; CLAUDIN 1; COLLAGEN TYPE 1; PROTEIN ZO1; VIRUS RNA; BIOLOGICAL MARKER; COLLAGEN;

ARTICLE; BIOREACTOR; CELL DIFFERENTIATION; CELL SPECIFICITY; CELL STRAIN CACO 2; CELL STRAIN INT 407; CONTROLLED STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; ELECTRON MICROSCOPY; EMBRYO; GENE DOSAGE; GENOTYPE; HUMAN; HUMAN CELL; IN VITRO STUDY; INCUBATION TIME; INOCULATION; INTESTINE EPITHELIUM CELL; MICROVILLUS; NONHUMAN; NOROVIRUS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; ROTATING BIOREACTOR; STAINING; SUPERNATANT; THREE DIMENSIONAL ORGANOID INTESTINAL CELL CULTURE MODEL; VIRUS CELL INTERACTION; VIRUS CULTURE; VIRUS LOAD; VIRUS REPLICATION; VIRUS STRAIN; BIOLOGICAL MODEL; CALICIVIRUS INFECTION; CELL SHAPE; CYTOLOGY; CYTOPLASM; GROWTH, DEVELOPMENT AND AGING; IMMUNOHISTOCHEMISTRY; INTESTINE; METABOLISM; PATHOLOGY; PROCEDURES; TIGHT JUNCTION; ULTRASTRUCTURE; VIROLOGY;

NOROVIRUS;

BIOLOGICAL MARKERS; CALICIVIRIDAE INFECTIONS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL SHAPE; COLLAGEN; HUMANS; IMMUNOHISTOCHEMISTRY; INTESTINES; MICROVILLI; MODELS, BIOLOGICAL; NOROVIRUS; ORGANOIDS; TIGHT JUNCTIONS; VIRUS REPLICATION;

EID: 84878659726     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0063485     Document Type: Article

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