Defining the functional potential and active community members of a sediment microbial community in a high-arctic hypersaline subzero spring

Applied and Environmental Microbiology

Volumn 79, Issue 12, 2013, Pages 3637-3648

  Lay, Chih Ying ^a   Mykytczuk, Nadia C S ^a   Yergeau, Étienne ^b   Lamarche Gagnon, Guillaume ^a   Greer, Charles W ^b   Whyte, Lyle G ^a  

^a MCGILL UNIVERSITY   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

METAGENOMIC ANALYSIS; MICROBIAL COMMUNITIES; MICROBIAL COMPONENTS; MICROBIAL POPULATIONS; OLIGOTROPHIC CONDITIONS; PERMAFROST ENVIRONMENT; STRESS RESPONSE GENES; SUB-ZERO TEMPERATURES;

AMMONIA; GENES; METABOLISM; METHANE; MICROORGANISMS; RNA; SULFUR;

SULFUR COMPOUNDS;

COMPLEMENTARY DNA; PRIMER DNA; RNA 16S;

ADAPTATION; DISCHARGE; ENVIRONMENTAL STRESS; ENZYME ACTIVITY; FLUVIAL DEPOSIT; GENETIC ANALYSIS; HYPERSALINE ENVIRONMENT; METABOLISM; MICROBIAL COMMUNITY; NUTRIENT CYCLING; OXIDATION; POPULATION STRUCTURE; TERRESTRIAL ECOSYSTEM;

ARCHAEON; ARCTIC; ARTICLE; BACTEROIDETES; COLD; CYANOBACTERIUM; DNA SEQUENCE; GENETICS; METAGENOME; MICROBIOLOGY; MOLECULAR GENETICS; NATURAL SPRING; NUCLEOTIDE SEQUENCE; PROTEOBACTERIA; SALINITY; SEDIMENT;

ARCHAEA; ARCTIC REGIONS; BACTEROIDETES; BASE SEQUENCE; COLD TEMPERATURE; CYANOBACTERIA; DNA PRIMERS; DNA, COMPLEMENTARY; GEOLOGIC SEDIMENTS; METAGENOME; MOLECULAR SEQUENCE DATA; NATURAL SPRINGS; PROTEOBACTERIA; RNA, RIBOSOMAL, 16S; SALINITY; SEQUENCE ANALYSIS, DNA;

CANADA;

BACTERIA (MICROORGANISMS); BACTEROIDETES; CYANOBACTERIA; DESULFOBULBUS; PROTEOBACTERIA; PSEUDOMONAS; THERMOPROTEI;

EID: 84878555073     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00153-13     Document Type: Article

References (99)

1. Varin T, Lovejoy C, Jungblut AD, Vincent WF, Corbeil J. 2012. Metagenomic analysis of stress genes in microbial mat communities from Antarctica and the high Arctic. Appl. Environ. Microbiol. 78:549-559.
2. Steven B, Pollard WH, Greer CW, Whyte LG. 2008. Microbial diversity and activity through a permafrost/ground ice core profile from the Canadian high Arctic. Environ. Microbiol. 10:3388-3403.
3. Lay CY, Mykytczuk NC, Niederberger TD, Martineau C, Greer CW, Whyte LG. 2012. Microbial diversity and activity in hypersaline high Arctic spring channels. Extremophiles 16:177-191.
4. Niederberger TD, Perreault NN, Tille S, Lollar BS, Lacrampe-Couloume G, Andersen D, Greer CW, Pollard W, Whyte LG. 2010. Microbial characterization of a subzero, hypersaline methane seep in the Canadian high Arctic. ISME J. 4:1326-1339.
5. Cary SC, McDonald IR, Barrett JE, Cowan DA. 2010. On the rocks: the microbiology of Antarctic Dry Valley soils. Nat. Rev. Microbiol. 8:129-138.
6. Bowman JS, Rasmussen S, Blom N, Deming JW, Rysgaard S, Sicheritz-Ponten T. 2012. Microbial community structure of Arctic multiyear sea ice and surface seawater by 454 sequencing of the 16S RNA gene. ISME J. 6:11-20.
7. Perreault NN, Andersen DT, Pollard WH, Greer CW, Whyte LG. 2007. Characterization of the prokaryotic diversity in cold saline perennial springs of the Canadian high Arctic. Appl. Environ. Microbiol. 73:1532-1543.
8. Perreault NN, Greer CW, Andersen DT, Tille S, Lacrampe-Couloume G, Lollar BS, Whyte LG. 2008. Heterotrophic and autotrophic microbial populations in cold perennial springs of the high Arctic. Appl. Environ. Microbiol. 74:6898-6907.
9. Pollard W, Haltigin T, Whyte L, Niederberger T, Andersen D, Omelon C, Nadeau J, Ecclestone M, Lebeuf M. 2009. Overview of analogue science activities at the McGill Arctic Research Station, Axel Heiberg Island, Canadian High Arctic. Planet Space Sci. 57:646-659.
10. Andersen DT, Pollard WH, McKay CP, Heldmann J. 2002. Cold springs in permafrost on Earth and Mars. J. Geophys Res. Planets 107(E3):4-1-4-7.
11. Pollard W, Omelon C, Andersen D, McKay C. 1999. Perennial spring occurrence in the Expedition Fiord area of western Axel Heiberg Island, Canadian High Arctic. Can. J. Earth Sci. 36:105-120.
12. Grasby SE, Allen CC, Longazo TG, Lisle JT, Griffin DW, Beauchamp B. 2003. Supraglacial sulfur springs and associated biological activity in the Canadian high Arctic-signs of life beneath the ice. Astrobiology 3:583-596.
13. Reigstad LJ, Jorgensen SL, Lauritzen SE, Schleper C, Urich T. 2011. Sulfur-oxidizing chemolithotrophic Proteobacteria dominate the microbiota in high Arctic thermal springs on Svalbard. Astrobiology 11:665-678.
14. Gleeson DF, Williamson C, Grasby SE, Pappalardo RT, Spear JR, Templeton AS. 2011. Low temperature S-0 biomineralization at a supraglacial spring system in the Canadian High Arctic. Geobiology 9:360-375.
15. Steven B, Niederberger TD, Bottos EM, Dyen MR, Whyte LG. 2007. Development of a sensitive radiorespiration method for detecting microbial activity at subzero temperatures. J. Microbiol. Methods 71:275-280.
16. Gendrin A, Mangold N, Bibring JP, Langevin Y, Gondet B, Poulet F, Bonello G, Quantin C, Mustard J, Arvidson R, LeMouelic S. 2005. Sulfates in Martian layered terrains: the OMEGA/Mars Express view. Science 307:1587-1591.
17. Hecht MH, Kounaves SP, Quinn RC, West SJ, Young SMM, Ming DW, Catling DC, Clark BC, Boynton WV, Hoffman J, DeFlores LP, Gospodinova K, Kapit J, Smith PH. 2009. Detection of perchlorate and the soluble chemistry of Martian soil at the Phoenix lander site. Science 325: 64-67.
18. Allen CC, Oehler DZ. 2008. A case for ancient springs in Arabia Terra, Mars. Astrobiology 8:1093-1112.
19. Rossi AP, Neukum G, Pondrelli M, van Gasselt S, Zegers T, Hauber E, Chicarro A, Foing B. 22 August 2008. Large-scale spring deposits on Mars? J. Geophys. Res. Planets 113:E08016. doi:10.1029/2007JE003062.
20. Malin MC, Edgett KS, Posiolova LV, McColley SM, Dobrea EZ. 2006. Present-day impact cratering rate and contemporary gully activity on Mars. Science 314:1573-1577.
21. McEwen AS, Ojha L, Dundas CM, Mattson SS, Byrne S, Wray JJ, Cull SC, Murchie SL, Thomas N, Gulick VC. 2011. Seasonal flows on warm Martian slopes. Science 333:740-743.
22. Keppler F, Vigano I, McLeod A, Ott U, Fruchtl M, Rockmann T. 2012. Ultraviolet-radiation-induced methane emissions from meteorites and the Martian atmosphere. Nature 486:93-96.
23. Mumma MJ, Villanueva GL, Novak RE, Hewagama T, Bonev BP, Disanti MA, Mandell AM, Smith MD. 2009. Strong release of methane on Mars in northern summer 2003. Science 323:1041-1045.
24. Zahnle K, Freedman RS, Catling DC. 2011. Is there methane on Mars? Icarus 212:493-503.
25. Postberg F, Schmidt J, Hillier J, Kempf S, Srama R. 2011. A salt-water reservoir as the source of a compositionally stratified plume on Enceladus. Nature 474:620-622.
26. Yergeau E, Hogues H, Whyte LG, Greer CW. 2010. The functional potential of high Arctic permafrost revealed by metagenomic sequencing, qPCR and microarray analyses. ISME J. 4:1206-1214.
27. Mackelprang R, Waldrop MP, Deangelis KM, David MM, Chavarria KL, Blazewicz SJ, Rubin EM, Jansson JK. 2011. Metagenomic analysis of a permafrost microbial community reveals a rapid response to thaw. Nature 480:368-371.
28. Brazelton WJ, Baross JA. 2010. Metagenomic comparison of two Thiomicrospira lineages inhabiting contrasting deep-sea hydrothermal environments. PLoS One 5:e13530. doi:10.1371/journal.pone.0013530.
29. Xie W, Wang F, Guo L, Chen Z, Sievert SM, Meng J, Huang G, Li Y, Yan Q, Wu S, Wang X, Chen S, He G, Xiao X, Xu A. 2011. Comparative metagenomics of microbial communities inhabiting deep-sea hydrothermal vent chimneys with contrasting chemistries. ISME J. 5:414-426.
30. Brazelton WJ, Baross JA. 2009. Abundant transposases encoded by the metagenome of a hydrothermal chimney biofilm. ISME J. 3:1420-1424.
31. Lauro FM, DeMaere MZ, Yau S, Brown MV, Ng C, Wilkins D, Raftery MJ, Gibson JAE, Andrews-Pfannkoch C, Lewis M, Hoffman JM, Thomas T, Cavicchioli R. 2011. An integrative study of a meromictic lake ecosystem in Antarctica. ISME J. 5:879-895.
32. Ng C, DeMaere MZ, Williams TJ, Lauro FM, Raftery MJ, Gibson JAE, Andrews-Pfannkoch C, Lewis M, Hoffman JM, Thomas T, Cavicchioli R. 2010. Metaproteogenomic analysis of a dominant green sulfur bacterium from Ace Lake, Antarctica. ISME J. 4:1002-1019.
33. Rodriguez-Blanco A, Ghiglione JF, Catala P, Casamayor EO, Lebaron P. 2009. Spatial comparison of total vs. active bacterial populations by coupling genetic fingerprinting and clone library analyses in theNWMediterranean Sea. FEMS Microbiol. Ecol. 67:30-42.
34. Burow LC, Woebken D, Bebout BM, McMurdie PJ, Singer SW, Pett-Ridge J, Prufert-Bebout L, Spormann AM, Weber PK, Hoehler TM. 2012. Hydrogen production in photosynthetic microbial mats in the Elkhorn Slough estuary, Monterey Bay. ISME J. 6:863-874.
35. Jones SE, Lennon JT. 2010. Dormancy contributes to the maintenance of microbial diversity. Proc. Natl. Acad. Sci. U. S. A. 107:5881-5886.
36. oC in the brine of an ice-sealed Antarctic lake. Proc. Natl. Acad. Sci. U. S. A. 109:20626-20631.
37. Meyer F, Paarmann D, D'Souza M, Olson R, Glass EM, Kubal M, Paczian T, Rodriguez A, Stevens R, Wilke A, Wilkening J, Edwards RA. 2008. The metagenomics RAST server-a public resource for the automatic phylogenetic and functional analysis of metagenomes. BMC Bioinformatics 9:386. doi:10.1186/1471-2105-9-386.
38. Kent WJ. 2002. BLAT-the BLAST-like alignment tool. Genome Res. 12:656-664.
39. Huson DH, Mitra S, Ruscheweyh HJ, Weber N, Schuster SC. 2011. Integrative analysis of environmental sequences using MEGAN4. Genome Res. 21:1552-1560.
40. Handl S, Dowd SE, Garcia-Mazcorro JF, Steiner JM, Suchodolski JS. 2011. Massive parallel 16S rRNA gene pyrosequencing reveals highly diverse fecal bacterial and fungal communities in healthy dogs and cats. FEMS Microbiol. Ecol. 76:301-310.
41. Baker GC, Smith JJ, Cowan DA. 2003. Review and re-analysis of domainspecific 16S primers. J. Microbiol. Methods 55:541-555.
42. Burggraf S, Huber H, Stetter KO. 1997. Reclassification of the crenarchaeal orders and families in accordance with 16S rRNA sequence data. Int. J. Syst. Bacteriol. 47:657-660.
43. Bailey MT, Dowd SE, Parry NMA, Galley JD, Schauer DB, Lyte M. 2010. Stressor exposure disrupts commensal microbial populations in the intestines and leads to increased colonization by Citrobacter rodentium. Infect. Immun. 78:1509-1519.
44. Cole JR, Wang Q, Cardenas E, Fish J, Chai B, Farris RJ, Kulam-Syed-Mohideen AS, McGarrell DM, Marsh T, Garrity GM, Tiedje JM. 2009. The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res. 37(Database issue):D141-D145.
45. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
46. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28:2731-2739.
47. Keegan KP, Trimble WL, Wilkening J, Wilke A, Harrison T, D'Souza M, Meyer F. 2012. A platform-independent method for detecting errors in metagenomic sequencing data: DRISEE. PLoS Comput. Biol. 8:e1002541. doi:10.1371/journal.pcbi.1002541.
48. Walter-Anthony KM, Anthony P, Grosse G, Chanton J. 2012. Geologic methane seeps along boundaries of Arctic permafrost thaw and melting glaciers. Nat. Geosci. 5:419-426.
49. Rivkina E, Laurinavichius K, McGrath J, Tiedje J, Shcherbakova V, Gilichinsky D. 2004. Microbial life in permafrost. Adv. Space Res. 33: 1215-1221.
50. Oren A. 2011. Thermodynamic limits to microbial life at high salt concentrations. Environ. Microbiol. 13:1908-1923.
51. Tazaz AM, Bebout BM, Kelley CA, Poole J, Chanton JP. 15 June 2012. Redefining the isotopic boundaries of biogenic methane: methane from endoevaporites. Icarus. doi:10.1016/j.icarus.2012.06.008.
52. Meyerdierks A, Kube M, Kostadinov I, Teeling H, Glockner FO, Reinhardt R, Amann R. 2010. Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group. Environ. Microbiol. 12:422-439.
53. Teske A, Biddle JF. 2008. Analysis of deep subsurface microbial communities by functional genes and genomics, p 159-176. In Dilek Y, Furnes H, Muehlenbachs K (ed), Modern approaches in solid earth sciences, vol 4. Links between geological processes, microbial activities&evolution of life. Springer, Berlin, Germany.
54. Steward GF, Zehr JP, Jellison R, Montoya JP, Hollibaugh JT. 2004. Vertical distribution of nitrogen-fixing phylotypes in a meromictic, hypersaline lake. Microb. Ecol. 47:30-40.
55. Ferrer M, Guazzaroni ME, Richter M, Garcia-Salamanca A, Yarza P, Suarez-Suarez A, Solano J, Alcaide M, van Dillewijn P, Molina-Henares MA, Lopez-Cortes N, Al-Ramahi Y, Guerrero C, Acosta A, de Eugenio LI, Martinez V, Marques S, Rojo F, Santero E, Genilloud O, Perez-Perez J, Rossello-Mora R, Ramos JL. 2011. Taxonomic and functional metagenomic profiling of the microbial community in the anoxic sediment of a sub-saline shallow lake (Laguna de Carrizo, Central Spain). Microb. Ecol. 62:824-837.
56. Auclair J, Parent S, Villemur R. 2012. Functional diversity in the denitrifying biofilm of the methanol-fed marine denitrification system at the Montreal Biodome. Microb. Ecol. 63:726-735.
57. Qu JH, Yuan HL, Li HF, Deng CP. 2009. Flavobacterium cauense sp. nov., isolated from sediment of a eutrophic lake. Int. J. Syst. Evol. Microbiol. 59:2666-2669.
58. Vernotte C, Picaud M, Kirilovsky D, Olive J, Ajlani G, Astier C. 1992. Changes in the photosynthetic apparatus in the cyanobacterium Synechocystis sp. PCC 6714 following light-to-dark and dark-to-light transitions. Photosynth. Res. 32:42-57.
59. Reddy KJ, Haskell JB, Sherman DM, Sherman LA. 1993. Unicellular, aerobic nitrogen-fixing Cyanobacteria of the genus Cyanothece. J. Bacteriol. 175:1284-1292.
60. Venterea RT. 2007. Nitrite-driven nitrous oxide production under aerobic soil conditions: kinetics and biochemical controls. Global Change Biol. 13:1798-1809.
61. Nubel U, Bateson MM, Madigan MT, Kuhl M, Ward DM. 2001. Diversity and distribution in hypersaline microbial mats of bacteria related to Chloroflexus spp. Appl. Environ. Microbiol. 67:4365-4371.
62. Kompantseva EI, Sorokin DY, Gorlenko VM, Namsaraev BB. 2005. The phototrophic community found in Lake Khilganta (an alkaline saline lake located in the southeastern Transbaikal region). Microbiology 74:352-361.
63. Kelly DP, Shergill JK, Lu WP, Wood AP. 1997. Oxidative metabolism of inorganic sulfur compounds by bacteria. Antonie Van Leeuwenhoek 71: 95-107.
64. Klotz MG, Arp DJ, Chain PSG, El-Sheikh AF, Hauser LJ, Hommes NG, Larimer FW, Malfatti SA, Norton JM, Poret-Peterson AT, Vergez LM, Ward BB. 2006. Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707. Appl. Environ. Microbiol. 72:6299-6315.
65. Feller G, Gerday C. 2003. Psychrophilic enzymes: hot topics in cold adaptation. Nat. Rev. Microbiol. 1:200-208.
66. Kawakami R, Sakuraba H, Kamohara S, Goda S, Kawarabayasi Y, Ohshima T. 2004. Oxidative stress response in an anaerobic hyperthermophilic archaeon: presence of a functional peroxiredoxin in Pyrococcus horikoshii. J. Biochem. 136:541-547.
67. Jean D, Briolat V, Reysset G. 2004. Oxidative stress response in Clostridium perfringens. Microbiology 150:1649-1659.
68. Briolat V, Reysset G. 2002. Identification of the Clostridium perfringens genes involved in the adaptive response to oxidative stress. J. Bacteriol. 184:2333-2343.
69. Rocha ER, Selby T, Coleman JP, Smith CJ. 1996. Oxidative stress response in an anaerobe, Bacteroides fragilis: a role for catalase in protection against hydrogen peroxide. J. Bacteriol. 178:6895-6903.
70. Srivastava AK. 2010. Assessment of salinity-induced antioxidative defense system of diazotrophic cyanobacterium Nostoc muscorum. J. Microbiol. Biotechnol. 20:1506-1512.
71. Latifi A, Ruiz M, Zhang CC. 2009. Oxidative stress in Cyanobacteria. FEMS Microbiol. Rev. 33:258-278.
72. Chattopadhyay MK. 2002. Low temperature and oxidative stress. Curr. Sci. 83:109. (Letter.)
73. Bohin JP. 2000. Osmoregulated periplasmic glucans in Proteobacteria. FEMS Microbiol. Lett. 186:11-19.
74. Sleator RD, Hill C. 2002. Bacterial osmoadaptation: the role of osmolytes in bacterial stress and virulence. FEMS Microbiol. Rev. 26:49-71.
75. Ting L, Williams TJ, Cowley MJ, Lauro FM, Guilhaus M, Raftery MJ, Cavicchioli R. 2010. Cold adaptation in the marine bacterium, Sphingopyxis alaskensis, assessed using quantitative proteomics. Environ. Microbiol. 12:2658-2676.
76. Panoff J-M, Thammavongs B, Laplace J-M, Hartke A, Boutibonnes P, Auffray Y. 1995. Cryotolerance and cold adaptation in Lactococcus lactis subsp. lactis IL1403. Cryobiology 32:516-520.
77. Atlung T, Hansen FG. 1999. Low-temperature-induced DnaA protein synthesis does not change initiation mass in Escherichia coli K-12. J. Bacteriol. 181:5557-5562.
78. Merrin J, Kumar P, Libchaber A. 2011. Effects of pressure and temperature on the binding of RecA protein to single-stranded DNA. Proc. Natl. Acad. Sci. U. S. A. 108:19913-19918.
79. Yamanaka K. 1999. Cold shock response in Escherichia coli. J. Mol. Microbiol. Biotechnol. 1:193-202.
80. Bakermans C, Bergholz PW, Ayala-del-Río H, Tiedje J. 2009. Genomic insights into cold adaptation of permafrost bacteria. Soil Biol. 16:159-168.
81. Hunger K, Beckering CL, Wiegeshoff F, Graumann PL, Marahiel MA. 2006. Cold-induced putative DEAD box RNA helicases CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus subtilis. J. Bacteriol. 188:240-248.
82. Py B, Higgins CF, Krisch HM, Carpousis AJ. 1996. A DEAD-box RNA helicase in the Escherichia coli RNA degradosome. Nature 381:169-172.
83. Ray S, Lee C, Hou TY, Bhakat KK, Brasier AR. 2010. Regulation of signal transducer and activator of transcription 3 enhanceosome formation by apurinic/apyrimidinic endonuclease 1 in hepatic acute phase response. Mol. Endocrinol. 24:391-401.
84. Jones PG, Mitta M, Kim Y, Jiang WN, Inouye M. 1996. Cold shock induces a major ribosomal-associated protein that unwinds doublestranded RNA in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 93:76-80.
85. Cavicchioli R, Thomas T, Curmi PM. 2000. Cold stress response in Archaea. Extremophiles 4:321-331.
86. de la Cruz J, Kressler D, Linder P. 1999. Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem. Sci. 24:192-198.
87. Suzuki Y, Haruki M, Takano K, Morikawa M, Kanaya S. 2004. Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptation. Eur. J. Biochem. 271: 1372-1381.
88. Scherer S, Neuhaus K. 2006. Life at low temperatures, p 210-262. In Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E (ed), The Prokaryotes, 3rd ed, vol 2. Ecophysiology and biochemistry. Springer Science, New York, NY.
89. Wouters JA, Rombouts FM, Kuipers OP, de Vos WM, Abee T. 2001. The role of cold-shock proteins in low-temperature adaptation, p 43-56. In Storey KB, Storey JM (ed), Protein adaptations and signal transduction. Elsevier Science, Amsterdam, Netherlands.
90. Rodrigues DF, Tiedje JM. 2008. Coping with our cold planet. Appl. Environ. Microbiol. 74:1677-1686.
91. Kenny JG, Ward D, Josefsson E, Jonsson IM, Hinds J, Rees HH, Lindsay JA, Tarkowski A, Horsburgh MJ. 2009. The Staphylococcus aureus response to unsaturated long chain free fatty acids: survival mechanisms and virulence implications. PLoS One 4:e4344. doi:10.1371/journal.pone.0004344.
92. Thieringer HA, Jones PG, Inouye M. 1998. Cold shock and adaptation. Bioessays 20:49-57.
93. Nicholson WL, Munakata N, Horneck G, Melosh HJ, Setlow P. 2000. Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiol. Mol. Biol. Rev. 64:548-572.
94. Sachidanandham R, Yew-Hoong Gin K. 2009. A dormancy state in nonspore-forming bacteria. Appl. Microbiol. Biotechnol. 81:927-941.
95. Brazelton WJ, Schrenk MO, Kelley DS, Baross JA. 2006. Methane-and sulfur-metabolizing microbial communities dominate the Lost City hydrothermal field ecosystem. Appl. Environ. Microbiol. 72:6257-6270.
96. Brochier-Armanet C, Boussau B, Gribaldo S, Forterre P. 2008. Mesophilic crenarchaeota: proposal for a third archaeal phylum, the Thaumarchaeota. Nat. Rev. Microbiol. 6:245-252.
97. Itoh T, Suzuki K, Nakase T. 2002. Vulcanisaeta distributa gen. nov., sp. nov., and Vulcanisaeta souniana sp. nov., novel hyperthermophilic, rodshaped crenarchaeotes isolated from hot springs in Japan. Int. J. Syst. Evol. Microbiol. 52:1097-1104.
98. Strauss G, Eisenreich W, Bacher A, Fuchs G. 1992. 13C-NMR study of autotrophic CO2 fixation pathways in the sulfur-reducing Archaebacterium Thermoproteus neutrophilus and in the phototrophic Eubacterium Chloroflexus aurantiacus. Eur. J. Biochem. 205:853-866.
99. Selig M, Schönheit P. 1994. Oxidation of organic compounds to CO2 with sulfur or thiosulfate as electron acceptor in the anaerobic hyperthermophilic archaea Thermoproteus tenaxT and Pyrobaculum islandicum proceeds via the citric acid cycle. Arch. Microbiol. 162:286-294.