A TaqMan-based multiplex real-time PCR assay for the simultaneous detection of Wuchereria bancrofti and Brugia malayi

Molecular and Biochemical Parasitology

Volumn 189, Issue 1-2, 2013, Pages 33-37

  Pilotte, N ^a,b   Torres, M ^a   Tomaino, F R ^a   Laney, S J ^a,d   Williams, S A ^a,c  

^a SMITH COLLEGE   (United States)

^b Division of Humanities and Fine Arts   (United States)

^c UNIVERSITY OF MASSACHUSETTS   (United States)

^d BILL AND MELINDA GATES FOUNDATION   (United States)

Author keywords

Brugia malayi; Diagnosis; Filariasis; Multiplex; PCR; Real time; Wuchereria bancrofti

Indexed keywords

ARTICLE; BRUGIA MALAYI; COST CONTROL; DISEASE ERADICATION; DNA EXTRACTION; ENDEMIC DISEASE; LYMPHATIC FILARIASIS; MOLECULAR DIAGNOSIS; MOSQUITO; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; WUCHERERIA BANCROFTI;

ANIMALS; BLOOD; BRUGIA MALAYI; CULICIDAE; DNA, HELMINTH; ELEPHANTIASIS, FILARIAL; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; PARASITOLOGY; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; WUCHERERIA BANCROFTI;

BRUGIA; BRUGIA MALAYI; WUCHERERIA BANCROFTI;

EID: 84878256037     PISSN: 01666851     EISSN: 18729428     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2013.05.001     Document Type: Article

References (15)

1. World Health Organization. http://www.who.int/mediacentre/factsheets/ fs102/en/; 2012 [accessed 28.09.12].
2. M.J. Bockarie, L. Kelly-Hope, and J.E. Haskew 10 years of global efforts to eliminate LF: where are we? Journal of Lymphoedema 5 2010 119 122
3. M.J. Bockarie, and D.H. Molyneux The end of lymphatic filariasis? British Medical Journal 338 2009 1470 1472
4. S.J. Joseph, and T.D. Read Bacterial population genomics and infectious disease diagnostics Trends in Biotechnology 28 2010 611 618
5. K.L. Muldrew Molecular diagnostics of infectious diseases Current Opinion in Pediatrics 21 2009 102 111
6. R.U. Rao, L.J. Atkinson, R.M.R. Ramzy, H. Helmy, H.A. Farid, and M.J. Bockarie A real-time PCR-based assay for detection of Wuchereria bancrofti DNA in blood and mosquitoes American Journal of Tropical Medicine and Hygiene 74 2006 826 832
7. R.U. Rao, G.J. Weil, K. Fischer, T. Supali, and P. Fischer Detection of Brugia parasite DNA in human blood by real-time PCR Journal of Clinical Microbiology 44 2006 3887 3893
8. R.U. Rao, S.A. Williams, and T.R. Klei Quantification of PCR amplification products of Brugia HhaI repeat DNA using a semiautomated Q-PCR system Molecular and Cellular Probes 16 2002 13 23
9. P.E. Simonsen Filariasis G.C. Cook, A. Zumla, Manson's tropical diseases 2003 Elsevier Science Limited Amsterdam 1487 1502
10. P.M. Intapan, T. Thanchomnang, V. Lulitanond, and W. Maleewong Rapid detection of Wuchereria bancrofti and Brugia malayi in mosquito vectors (Diptera: Culicidae) using a real-time fluorescence resonance energy transfer multiplex PCR and melting curve analysis Journal of Medical Entomology 46 2009 158 164
11. K. Gass, M.V.E.B. de Rochars, D. Boakye, M. Bradley, P.U. Fischer, and J. Gyapong A multicenter evaluation of diagnostic tools to define endpoints for programs to eliminate Bancroftian filariasis PLoS Neglected Tropical Diseases 6 2012 e1479
12. Lymphatic Filariasis Support Center Standard Operating Procedures Gates Program: "Resolving Critical Challenges Now Facing the Global Programme to Eliminate Lymphatic Filariasis", Objective #1 2012 www.filariasis.us/ resources.html [accessed 30.01.13]
13. R.C. Wilkerson, J.F. Reinert, and C. Li Ribosomal DNA ITS2 sequences differentiate six species in the Anopheles crucians complex (Diptera: Culicidae) Journal of Medical Entomology 41 2004 392 401
14. F. Coutleé, P. Gravitt, H. Richardson, C. Hankins, E. Franco, and N. Lapointe Nonisotopic detection and typing of human papillomavirus DNA in genital samples by the line blot assay Journal of Clinical Microbiology 37 1999 1852 1857
15. M.R. Lizotte, T. Supali, F. Partono, and S.A. Williams A polymerase chain reaction assay for the detection of Brugia malayi in blood American Journal of Tropical Medicine and Hygiene 51 1994 314 321