A simple skin blister technique for the study of in vivo transmigration of human leukocytes

Journal of Immunological Methods

Volumn 393, Issue 1-2, 2013, Pages 8-17

  Davidsson, Lisa ^a   Björkman, Lena ^a   Christenson, Karin ^a   Alsterholm, Mikael ^a   Movitz, Charlotta ^a   Thorén, Fredrik B ^a   Karlsson, Anna ^a   Welin, Amanda ^a   Bylund, Johan ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Cytokines; Exudate; Inflammation; Neutrophil; Transmigration

Indexed keywords

ANALYTIC METHOD; ARTICLE; BLISTER; CELL INFILTRATION; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; IN VIVO STUDY; INFLAMMATION; LEUKOCYTE MIGRATION; MEDIATOR RELEASE; PRIORITY JOURNAL;

BLISTER; CELL MOVEMENT; GRANULOCYTE COLONY-STIMULATING FACTOR; HUMANS; INFLAMMATION; LEUKOCYTES; PHAGOCYTOSIS; PHENOTYPE; SKIN; SKIN DISEASES;

EID: 84878123943     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.03.013     Document Type: Article

References (20)

1. Christenson K., Bjorkman L., Karlsson J., Sundqvist M., Movitz C., Speert D.P., Dahlgren C., Bylund J. In vivo-transmigrated human neutrophils are resistant to antiapoptotic stimulation. J. Leukoc. Biol. 2011, 90:1055.
2. Christenson K., Thoren F.B., Bylund J. Analyzing cell death events in cultured leukocytes. Methods Mol. Biol. 2012, 844:65.
3. Clemmensen S.N., Bohr C.T., Rorvig S., Glenthoj A., Mora-Jensen H., Cramer E.P., Jacobsen L.C., Larsen M.T., Cowland J.B., Tanassi J.T., Heegaard N.H., Wren J.D., Silahtaroglu A.N., Borregaard N. Olfactomedin 4 defines a subset of human neutrophils. J. Leukoc. Biol. 2012, 91:495.
4. Condliffe A.M., Kitchen E., Chilvers E.R. Neutrophil priming: pathophysiological consequences and underlying mechanisms. Clin. Sci. (Lond) 1998, 94:461.
5. Day R.M., Harbord M., Forbes A., Segal A.W. Cantharidin blisters: a technique for investigating leukocyte trafficking and cytokine production at sites of inflammation in humans. J. Immunol. Methods 2001, 257:213.
6. Follin P., Dahlgren C. A skin chamber technique as a human model for studies of aseptic inflammatory reactions. Methods Mol. Biol. 2007, 412:333.
7. Follin P., Wymann M.P., Dewald B., Ceska M., Dahlgren C. Human neutrophil migration into skin chambers is associated with production of NAP-1/IL8 and C5a. Eur. J. Haematol. 1991, 47:71.
8. Hennigan S.M., Wang J.H., Redmond H.P., Bouchier-Hayes D. Neutrophil heat shock protein expression and activation correlate with increased apoptosis following transmigration through the endothelial barrier. Shock 1999, 12:32.
9. Hustinx W.N., Van Kessel C.P., Heezius E., Burgers S., Lammers J.W., Hoepelman I.M. Effects of granulocyte colony-stimulating factor (G-CSF) treatment on granulocyte function and receptor expression in patients with ventilator-dependent pneumonia. Clin. Exp. Immunol. 1998, 112:334.
10. Karlsson A., Follin P., Leffler H., Dahlgren C. Galectin-3 activates the NADPH-oxidase in exudated but not peripheral blood neutrophils. Blood 1998, 91:3430.
11. Kiistala U., Mustakallio K.K. In-vivo separation of epidermis by production of suction blisters. Lancet 1964, 1:1444.
12. Kuhns D.B., Long Priel D.A., Gallin J.I. Loss of L-selectin (CD62L) on human neutrophils following exudation in vivo. Cell. Immunol. 1995, 164:306.
13. Lukac J., Mravak-Stipetic M., Knezevic M., Vrcek J., Sistig S., Ledinsky M., Kusic Z. Phagocytic functions of salivary neutrophils in oral mucous membrane diseases. J. Oral Pathol. Med. 2003, 32:271.
14. Paccaud J.P., Carpentier J.L., Schifferli J.A. Exudation induces clustering of CR1 receptors at the surface of human polymorphonuclear leukocytes. Biochem. Biophys. Res. Commun. 1990, 172:1203.
15. Paulsson J.M., Held C., Jacobson S.H., Lundahl J. In vivo extravasated human monocytes have an altered expression of CD16, HLA-DR, CD86, CD36 and CX(3)CR1. Scand. J. Immunol. 2009, 70:368.
16. Paulsson J.M., Jacobson S.H., Lundahl J. Neutrophil activation during transmigration in vivo and in vitro. A translational study using the skin chamber model. J. Immunol. Methods 2010, 361:82.
17. Seely A.J., Naud J.F., Campisi G., Giannias B., Liu S., DiCarlo A., Ferri L.E., Pascual J.L., Tchervenkov J., Christou N.V. Alteration of chemoattractant receptor expression regulates human neutrophil chemotaxis in vivo. Ann. Surg. 2002, 235:550.
18. Sengelov H., Follin P., Kjeldsen L., Lollike K., Dahlgren C., Borregaard N. Mobilization of granules and secretory vesicles during in vivo exudation of human neutrophils. J. Immunol. 1995, 154:4157.
19. Vignali D.A. Multiplexed particle-based flow cytometric assays. J. Immunol. Methods 2000, 243:243.
20. Zarember K.A., Kuhns D.B. Editorial: will the real neutrophil please stand up?. J. Leukoc. Biol. 2011, 90:1039.