Comparison of three different fat graft preparation methods: Gravity separation, centrifugation, and simultaneous washing with filtration in a closed system

Plastic and Reconstructive Surgery

Volumn 131, Issue 4, 2013, Pages 873-880

  Zhu, Min ^a,b   Cohen, Steven R ^a,b   Hicok, Kevin C ^a,b   Shanahan, Rob K ^a,b   Strem, Brian M ^a,b   Yu, Johnson C ^a,b   Arm, Douglas M ^a,b   Fraser, John K ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b RADY CHILDREN'S HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ARTICLE; CENTRIFUGATION; COMPARATIVE STUDY; FEMALE; FILTRATION; GRAVITY; HUMAN; METHODOLOGY; MIDDLE AGED; SUBCUTANEOUS FAT; TRANSPLANTATION;

ADULT; CENTRIFUGATION; FEMALE; FILTRATION; GRAVITATION; HUMANS; MIDDLE AGED; SUBCUTANEOUS FAT; TISSUE AND ORGAN HARVESTING; YOUNG ADULT;

MLCS; MLOWN;

EID: 84878100847     PISSN: 00321052     EISSN: None     Source Type: Journal    
DOI: 10.1097/PRS.0b013e31828276e9     Document Type: Article

References (19)

1. Kaufman MR, Miller TA, Huang C, et al. Autologous fat transfer for facial recontouring: Is there science behind the art? Plast Reconstr Surg. 2007;119:2287-2296.
2. Shiffman MA. History of autologous fat transplant survival. In: Shiffman MA, ed Autologous Fat Transfer: Art, Science, and Clinical Practice. Heidelberg: Springer-Verlag; 2010:5-10.
3. Chan CW, McCulley SJ, Macmillan RD. Autologous fat transfer: A review of the literature with a focus on breast cancer surgery. J Plast Reconstr Aesthet Surg. 2008;61:1438-1448.
4. Coleman SR. Structural fat grafts: the ideal filler? Clin Plast Surg. 2001;28:111-119.
5. Conde-Green A, de Amorim NF, Pitanguy I. Influence of decantation, washing and centrifugation on adipocyte and mesenchymal stem cell content of aspirated adipose tissue: A comparative study. J Plast Reconstr Aesthet Surg. 2010;63: 1375-1381.
6. Botti G, Pascali M, Botti C, Bodog F, Cervelli V. A clinical trial in facial fat grafting: Filtered and washed versus centrifuged fat. Plast Reconstr Surg. 2011;127:2464-2473.
7. Kaufman MR, Bradley JP, Dickinson B, et al. Autologous fat transfer national consensus survey: Trends in techniques for harvest, preparation, and application, and perception of short-and long-term results. Plast Reconstr Surg. 2007;119: 323-331.
8. Coleman SR. Structural fat grafting: More than a permanent filler. Plast Reconstr Surg. 2006;118(3 Suppl):108S-120S.
9. Pu LL. Towards more rationalized approach to autologous fat grafting. J Plast Reconstr Aesthet Surg. 2012;65:413-419.
10. Kurita M, Matsumoto D, Shigeura T, et al. Influences of centrifugation on cells and tissues in liposuction aspirates: Optimized centrifugation for lipotransfer and cell isolation. Plast Reconstr Surg. 2008;121:1033-1041.
11. Pu LL, Coleman SR, Cui X, Ferguson RE Jr, Vasconez HC. Autologous fat grafts harvested and refined by the Coleman technique: A comparative study. Plast Reconstr Surg. 2008; 122:932-937.
12. Rohrich RJ, Sorokin ES, Brown SA. In search of improved fat transfer viability: A quantitative analysis of the role of centrifugation and harvest site. Plast Reconstr Surg. 2004;113:391-395.
13. Eppler SM, Combs DL, Henry TD, et al. A target-mediated model to describe the pharmacokinetics and hemodynamic effects of recombinant human vascular endothelial growth factor in humans. Clin Pharmacol Ther. 2002;72:20-32.
14. Bowen-Pope DF, Malpass TW, Foster DM, Ross R. Plateletderived growth factor in vivo: Levels, activity, and rate of clearance. Blood 1984;64:458-469.
15. Whalen GF, Shing Y, Folkman J. The fate of intravenously administered bFGF and the effect of heparin. Growth Factors 1989;1:157-1564.
16. Nishimura T, Hashimoto H, Nakanishi I, Furukawa M. Microvascular angiogenesis and apoptosis in the survival of free fat grafts. Laryngoscope 2000;110:1333-1338.
17. Smith P, Adams WP Jr, Lipschitz AH, et al. Autologous human fat grafting: Effect of harvesting and preparation techniques on adipocyte graft survival. Plast Reconstr Surg. 2006; 117:1836-1844.
18. Xie Y, Zheng D, Li Q, Chen Y, Lei H, Pu LL. The effect of centrifugation on viability of fat grafts: An evaluation with the glucose transport test. J Plast Reconstr Aesthet Surg. 2010;63: 482-487.
19. Minn KW, Min KH, Chang H, Kim S, Heo EJ. Effects of fat preparation methods on the viabilities of autologous fat grafts. Aesthetic Plast Surg. 2010;34:626-631.