Determination of stage interconversion in vitro and in vivo by construction of transgenic Toxoplasma gondii that stably express stage-specific fluorescent proteins

Experimental Parasitology

Volumn 134, Issue 3, 2013, Pages 275-280

  Zhang, Houshuang ^a   Zhang, Yanlei ^a   Cao, Jie ^a   Zhou, Yongzhi ^a   Wang, Na ^a   Zhou, Jinlin ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Bradyzoit; GFP; RFP; Stage conversion; Tachyzoit; Toxoplasma gondii

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; FLUORESCENT DYE; RECOMBINANT PROTEIN; RED FLUORESCENCE PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; APICOMPLEXAN LIFE CYCLE STAGE; ARTICLE; BAG1 GENE; BRADYZOITE; CELL VACUOLE; CONTROLLED STUDY; FEMALE; FLUORESCENCE ANALYSIS; GENE; IN VITRO STUDY; IN VIVO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SAG1 GENE; TACHYZOITE; TOXOPLASMA GONDII; TRANSGENE; VIRULENCE;

ANIMALS; BRAIN; CERCOPITHECUS AETHIOPS; ELECTROPORATION; FEMALE; FIBROBLASTS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MICE; MICE, INBRED BALB C; MICROSCOPY, FLUORESCENCE; ORGANISMS, GENETICALLY MODIFIED; PROTOZOAN PROTEINS; SPECIES SPECIFICITY; TOXOPLASMA; TOXOPLASMOSIS, ANIMAL; TRANSFECTION; VACUOLES; VERO CELLS; VIRULENCE;

EID: 84877833563     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2013.03.015     Document Type: Article

References (31)

1. Bhadra R., Gigley J.P., Khan I.A. The CD8 T-cell road to immunotherapy of toxoplasmosis. Immunotherapy 2011, 3:789-801.
2. Blader I.J., Saeij J.P. Communication between Toxoplasma gondii and its host: impact on parasite growth, development, immune evasion, and virulence. APMIS 2009, 117:458-476.
3. Bohne W., Heesemann J., Gross U. Induction of bradyzoite-specific Toxoplasma gondii antigens in gamma interferon-treated mouse macrophages. Infect. Immun. 1993, 61:1141-1145.
4. Bohne W., Hunter C.A., White M.W., Ferguson D.J., Gross U., Roos D.S. Targeted disruption of the bradyzoite-specific gene BAG1 does not prevent tissue cyst formation in Toxoplasma gondii. Mol. Biochem. Parasitol. 1998, 92:291-301.
5. Carruthers V.B., Suzuki Y. Effects of Toxoplasma gondii infection on the brain. Schizophr. Bull. 2007, 33:745-751.
6. Charest H., Sedegah M., Yap G.S., Gazzinelli R.T., Caspar P., Hoffman S.L., Sher A. Recombinant attenuated Toxoplasma gondii expressing the Plasmodium yoelii circumsporozoite protein provides highly effective priming for CD8+ T cell-dependent protective immunity against malaria. J. Immunol. 2000, 165:2084-2092.
7. Di Cristina M., Ghouze F., Kocken C.H., Naitza S., Cellini P., Soldati D., Thomas A.W., Crisanti A. Transformed Toxoplasma gondii tachyzoites expressing the circumsporozoite protein of Plasmodium knowlesi elicit a specific immune response in rhesus monkeys. Infect. Immun. 1999, 67:1677-1682.
8. Di Cristina M., Marocco D., Galizi R., Proietti C., Spaccapelo R., Crisanti A. Temporal and spatial distribution of Toxoplasma gondii differentiation into bradyzoites and tissue cyst formation in vivo. Infect. Immun. 2008, 76:3491-3501.
9. Dzierszinski F., Nishi M., Ouko L., Roos D.S. Dynamics of Toxoplasma gondii differentiation. Eukaryot. Cell 2004, 3:992-1003.
10. Ferguson D.J. Use of molecular and ultrastructural markers to evaluate stage conversion of Toxoplasma gondii in both the intermediate and definitive host. Int. J. Parasitol. 2004, 34:347-360.
11. Ferreira da Silva Mda F., Barbosa H.S., Gross U., Lüder C.G. Stress-related and spontaneous stage differentiation of Toxoplasma gondii. Mol. BioSyst. 2008, 4:824-834.
12. Fox B.A., Gigley J.P., Bzik D.J. Toxoplasma gondii lacks the enzymes required for de novo arginine biosynthesis and arginine starvation triggers cyst formation. Int. J. Parasitol. 2004, 34:323-331.
13. Ibrahim H.M., Bannai H., Xuan X., Nishikawa Y. Toxoplasma gondii cyclophilin 18-mediated production of nitric oxide induces bradyzoite conversion in a CCR5-dependent manner. Infect. Immun. 2009, 77:3686-3695.
14. Karsten V., Qi H., Beckers C.J., Reddy A., Dubremetz J.F., Webster P., Joiner K.A. The protozoan parasite Toxoplasma gondii targets proteins to dense granules and the vacuolar space using both conserved and unusual mechanisms. J. Cell Biol. 1998, 141:1323-1333.
15. Kim S.K., Karasov A., Boothroyd J.C. Bradyzoite-specific surface antigen SRS9 plays a role in maintaining Toxoplasma gondii persistence in the brain and in host control of parasite replication in the intestine. Infect. Immun. 2007, 75:1626-1634.
16. Lüder C.G., Algner M., Lang C., Bleicher N., Gross U. Reduced expression of the inducible nitric oxide synthase after infection with Toxoplasma gondii facilitates parasite replication in activated murine macrophages. Int. J. Parasitol. 2003, 33:833-844.
17. Lyons R.E., McLeod R., Roberts C.W. Toxoplasma gondii tachyzoite-bradyzoite interconversion. Trends Parasitol. 2002, 18:198-201.
18. Nishikawa Y., Zhang H., Ibrahim H.M., Ui F., Ogiso A., Xuan X. Construction of Toxoplasma gondii bradyzoite expressing the green fluorescent protein. Parasitol. Int. 2008, 57:219-222.
19. Nishikawa Y., Xuenan X., Makala L., Vielemeyer O., Joiner K.A., Nagasawa H. Characterisation of Toxoplasma gondii engineered to express mouse interferon-gamma. Int. J. Parasitol. 2003, 33:1525-1535.
20. Saeij J.P., Boyle J.P., Grigg M.E., Arrizabalaga G., Boothroyd J.C. Bioluminescence imaging of Toxoplasma gondii infection in living mice reveals dramatic differences between strains. Infect. Immun. 2005, 73:695-702.
21. Scheidegger A., Vonlaufen N., Naguleswaran A., Gianinazzi C., Müller N., Leib S.L., Hemphill A. Differential effects of interferon-gamma and tumor necrosis factor-alpha on Toxoplasma gondii proliferation in organotypic rat brain slice cultures. J. Parasitol. 2005, 91:307-315.
22. Sibley L.D., Messina M., Niesman I.R. Stable DNA transformation in the obligate intracellular parasite Toxoplasma gondii by complementation of tryptophan auxotrophy. Proc. Natl. Acad. Sci. USA 1994, 91:5508-5512.
23. Singh U., Brewer J.L., Boothroyd J.C. Genetic analysis of tachyzoite to bradyzoite differentiation mutants in Toxoplasma gondii reveals a hierarchy of gene induction. Mol. Microbiol. 2002, 44:721-733.
24. Soete M., Camus D., Dubremetz J.F. Experimental induction of bradyzoite-specific antigen expression and cyst formation by the RH strain of Toxoplasma gondii in vitro. Exp. Parasitol. 1994, 78:361-370.
25. Striepen B., He C.Y., Matrajt M., Soldati D., Roos D.S. Expression, selection, and organellar targeting of the green fluorescent protein in Toxoplasma gondii. Mol. Biochem. Parasitol. 1998, 92:325-338.
26. Takashima Y., Suzuki K., Xuan X., Nishikawa Y., Unno A., Kitoh K. Detection of the initial site of Toxoplasma gondii reactivation in brain tissue. Int. J. Parasitol. 2008, 38:601-607.
27. Unno A., Suzuki K., Batanova T., Cha S.Y., Jang H.K., Kitoh K., Takashima Y. Visualization of Toxoplasma gondii stage conversion by expression of stage-specific dual fluorescent proteins. Parasitology 2009, 136:579-588.
28. Vanchinathan P., Brewer J.L., Harb O.S., Boothroyd J.C., Singh U. Disruption of a locus encoding a nucleolar zinc finger protein decreases tachyzoite-to-bradyzoite differentiation in Toxoplasma gondii. Infect. Immun. 2005, 73:6680-6688.
29. Weiss L.M., Laplace D., Takvorian P.M., Tanowitz H.B., Cali A., Wittner M. A cell culture system for study of the development of Toxoplasma gondii bradyzoites. J. Eukaryot. Microbiol. 1995, 42:150-157.
30. Zhang H., Compaore M.K., Lee E.G., Liao M., Zhang G., Sugimoto C., Fujisaki K., Nishikawa Y., Xuan X. Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites. Mol. Biochem. Parasitol. 2007, 151:205-212.
31. Zhang H., Nishikawa Y., Yamagishi J., Zhou J., Ikehara Y., Kojima N., Yokoyama N., Xuan X. Neospora caninum: application of apical membrane antigen 1 encapsulated in the oligomannose-coated liposomes for reduction of offspring mortality from infection in BALB/c mice. Exp. Parasitol. 2010, 125:130-136.