Using HitAlert flow cytometry to detect heparin-induced thrombocytopenia antibodies in a tertiary care hospital

Blood Coagulation and Fibrinolysis

Volumn 24, Issue 4, 2013, Pages 365-370

  Solano, Connie ^a   Mutsando, Howard ^b   Self, Marlene ^a   Morel Kopp, Marie Christine ^c,d   Mollee, Peter ^a,e  

^a PRINCESS ALEXANDRA HOSPITAL   (Australia)

^b ROYAL BRISBANE AND WOMEN'S HOSPITAL   (Australia)

^c ROYAL NORTH SHORE HOSPITAL   (Australia)

^d UNIVERSITY OF SYDNEY   (Australia)

^e UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

flow cytometry; heparin induced thrombocytopenia; serotonin release assay; whole blood impedance aggregometry

Indexed keywords

ENOXAPARIN; HEPARIN; HEPARIN PLATELET FACTOR 4 ANTIBODY; THROMBOCYTE FACTOR 4;

ABDOMINAL AORTA ANEURYSM; ACUTE KIDNEY FAILURE; ADULT; AGED; ANEURYSM SURGERY; ARTICLE; CLINICAL ARTICLE; CLINICAL ASSESSMENT; CONTROLLED STUDY; DEEP VEIN THROMBOSIS; DIAGNOSTIC TEST ACCURACY STUDY; ENZYME ASSAY; FEMALE; FLOW CYTOMETRY; GEL; GOLD STANDARD; HEMATOLOGY TEST KIT; HEMODIALYSIS; HEPARIN INDUCED THROMBOCYTOPENIA; HIGH RISK POPULATION; HUMAN; IMMUNOASSAY; LUNG EMBOLISM; MALE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEROTONIN RELEASE; TERTIARY HEALTH CARE; THROMBOCYTOPENIA;

ADULT; AGED; AGED, 80 AND OVER; ANTICOAGULANTS; AUTOANTIBODIES; FEMALE; FLOW CYTOMETRY; HEPARIN; HUMANS; IMMUNOASSAY; MALE; MIDDLE AGED; PLATELET FACTOR 4; SENSITIVITY AND SPECIFICITY; SEROTONIN; TERTIARY CARE CENTERS; THROMBOCYTOPENIA; WHOLE BLOOD COAGULATION TIME;

EID: 84877722699     PISSN: 09575235     EISSN: 14735733     Source Type: Journal    
DOI: 10.1097/MBC.0b013e32835cc17e     Document Type: Article

References (25)

1. Warkentin TE. Heparin-induced thrombocytopenia: pathogenesis and management. Br J Haematol 2003;121:535-555. (Pubitemid 36645375)
2. Warkentin TE, Heddle NM. Laboratory diagnosis of immune heparininduced thrombocytopenia. Curr Hematol Rep 2003;2:148-157.
3. Morel-Kopp MC, Aboud M, Tan CW, Kulathilake C, Ward C. Whole blood impedance aggregometry detects heparin-induced thrombocytopenia antibodies. Thromb Res 2010;125:e234-e239.
4. Sheridan D, Carter C, Kelton JG. A diagnostic test for heparin-induced thrombocytopenia. Blood 1986;67:27-30. (Pubitemid 16167006)
5. Meyer O, Salama A, Pittet N, Schwind P. Rapid detection of heparininduced platelet antibodies with particle gel immunoassay (ID-HPF4). Lancet 1999;354:1525-1526. (Pubitemid 29502110)
6. Morel-Kopp MC, Tan CW, Brighton TA, McRae S, Baker R, Tran H, et al. Validation of whole blood impedance aggregometry as a new diagnostic tool for HIT: results of a large Australian study. Thromb Haemost 2012;107:575-583.
7. Warkentin TE, Levine MN, Hirsh J, Horsewood P, Roberts RS, GentM, et al. Heparin-induced thrombocytopenia in patients treated with low-molecularweight heparin or unfractionated heparin. N Engl J Med 1995;332:1330-1335.
8. Nand S, Wong W, Yuen B, Yetter A, Schmulbach E, Gross Fisher S. Heparin-induced thrombocytopenia with thrombosis: incidence, analysis of risk factors, and clinical outcomes in 108 consecutive patients treated at a single institution. Am J Hematol 1997;56:12-16. (Pubitemid 27398052)
9. Smythe MA, Warkentin TE, Woodhouse AL, Zakalik D. Venous limb gangrene and fatal hemorrhage: adverse consequences of HIT 'overdiagnosis' in a patient with antiphospholipid syndrome. Am J Hematol 2011;86:188-191.
10. Tomer A. A sensitive and specific functional flow cytometric assay for the diagnosis of heparin-induced thrombocytopenia. Br J Haematol 1997;98:648-656. (Pubitemid 27386546)
11. Jy W, Mao WW, Horstman LL, Valant PA, Ahn YS. A flow cytometric assay of platelet activation marker P-selectin (CD62P) distinguishes heparininduced thrombocytopenia (HIT) from HIT with thrombosis (HITT). Thromb Haemost 1999;82:1255-1259. (Pubitemid 29473788)
12. Kelton JG, Sheridan D, Brain H, Powers PJ, Turpie AG, Carter CJ. Clinical usefulness of testing for a heparin-dependent platelet-aggregating factor in patients with suspected heparin-associated thrombocytopenia. J Lab Clin Med 1984;103:606-612. (Pubitemid 14126081)
13. Poley S, Mempel W. Laboratory diagnosis of heparin-induced thrombocytopenia: advantages of a functional flow cytometric test in comparison to the heparin-induced platelet-activation test. Eur J Haematol 2001;66:253-262. (Pubitemid 32436740)
14. Lo GK, Sigouin CS, Warkentin TE. What is the potential for overdiagnosis of heparin-induced thrombocytopeniaAm J Hematol 2007;82:1037-1043. (Pubitemid 350209843)
15. Greinacher A, Michels I, Kiefel V, Mueller-Eckhardt C. A rapid and sensitive test for diagnosing heparin-associated thrombocytopenia. Thromb Haemost 1991;66:734-736.
16. Warkentin TE. New approaches to the diagnosis of heparin-induced thrombocytopenia. Chest 2005;127:35S-45S. (Pubitemid 40224080)
17. Warkentin TE. How I diagnose and manage HIT. Hematol Am Soc Hematol Educ Program 2011;2011:143-149.
18. Pouplard C, Gueret P, Fouassier M, Ternisien C, Trossaert M, Regina S, et al. Prospective evaluation of the '4Ts' score and particle gel immunoassay specific to heparin/PF4 for the diagnosis of heparin-induced thrombocytopenia. J Thromb Haemost 2007;5:1373-1379. (Pubitemid 46965362)
19. Lo GK, Juhl D, Warkentin TE, Sigouin CS, Eichler P, Greinacher A. Evaluation of pretest clinical score (4 T's) for the diagnosis of heparininduced thrombocytopenia in two clinical settings. J Thromb Haemost 2006;4:759-765.
20. Lee DH, Warkentin TE, Denomme GA, Hayward CP, Kelton JG. A diagnostic test for heparin-induced thrombocytopenia: detection of platelet microparticles using flow cytometry. Br J Haematol 1996;95:724-731. (Pubitemid 26423379)
21. Tawfik NM, Hegazy MA, Hassan EA, Ramadan YK, Nasr AS. Egyptian experience of reliability of 4T's score in diagnosis of heparin induced thrombocytopenia syndrome. Blood Coagul Fibrinolysis 2011;22:701-705.
22. Din F, Kovacs MJ, Butler R, Lazo-Landner A. Inter-observer variability in the assessment of the 4Ts score for the diagnosis of heparin-induced thrombocytopenia in patients undergoing cardiac surgery. Blood 2009;114:Abstract 4467.
23. Walenga JM, Jeske WP, Fasanella AR, Wood JJ, Bakhos M. Laboratory tests for the diagnosis of heparin-induced thrombocytopenia. Semin Thromb Hemost 1999;25 (Suppl 1):43-49. (Pubitemid 29230564)
24. Walenga JM, Jeske WP, Wood JJ, Ahmad S, Lewis BE, Bakhos M. Laboratory tests for heparin-induced thrombocytopenia: a multicenter study. Semin Hematol 1999;36:22-28. (Pubitemid 29045507)
25. Harenberg J, Wang LC, Hoffmann U, Huhle G, Feuring M. Laboratory diagnosis of heparin-induced thrombocytopenia type II after clearance of platelet factor 4/heparin complex. J Lab Clin Med 2001;137:408-413. (Pubitemid 32473619)