A chimeric UDP-Glucose pyrophosphorylase produced by protein engineering exhibits sensitivity to allosteric regulators

International Journal of Molecular Sciences

Volumn 14, Issue 5, 2013, Pages 9703-9721

  Asención Diez, Matías D ^a,b   Ebrecht, Ana C ^a,b   Martínez, Lucila I ^a   Aleanzi, Mabel C ^a   Guerrero, Sergio A ^a   Ballícora, Miguel A ^b   Iglesias, Alberto A ^a  

^a Universidad Nacional del Litoral   (Argentina)

^b LOYOLA UNIVERSITY CHICAGO   (United States)

Author keywords

ADP glucose; Allosteric regulation; Protein engineering; Pyrophosphorylases evolution; UDP glucose

Indexed keywords

2,3 DIPHOSPHOGLYCERIC ACID; FRUCTOSE 1,6 BISPHOSPHATE; PYRUVIC ACID; URIDINE TRIPHOSPHATE GLUCOSE 1 PHOSPHATE URIDYLYLTRANSFERASE; GLUCOSE 1 PHOSPHATE; GLUCOSE 1 PHOSPHATE ADENYLYLTRANSFERASE; GLUCOSE PHOSPHATE; HYBRID PROTEIN;

ALLOSTERISM; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME REGULATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ESCHERICHIA COLI; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DOMAIN; PROTEIN ENGINEERING; STREPTOCOCCUS MUTANS; STRUCTURAL HOMOLOGY; AMINO ACID SEQUENCE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS;

ALLOSTERIC REGULATION; AMINO ACID SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GLUCOSE-1-PHOSPHATE ADENYLYLTRANSFERASE; GLUCOSEPHOSPHATES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; STREPTOCOCCUS MUTANS; UTP-GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE;

EID: 84877719588     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms14059703     Document Type: Article

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