Combined multi-gene analysis at the RNA and protein levels in single FFPE tissue sections

Experimental and Molecular Pathology

Volumn 95, Issue 1, 2013, Pages 1-6

  Ostalecki, Christian ^a   Konrad, Andreas ^a   Thurau, Elisabeth ^a   Schuler, Gerold ^a   Croner, Roland S ^a   Pommer, Ansgar J ^a   Stürzl, Mich ael ^a  

^a UNIVERSITY HOSPITAL ERLANGEN   (Germany)

Author keywords

MELC; QRT PCR

Indexed keywords

B RAF KINASE; BETA CATENIN; CD34 ANTIGEN; CD45 ANTIGEN; KI 67 ANTIGEN; LYSOSOME ASSOCIATED MEMBRANE PROTEIN 1; NOTCH1 RECEPTOR; NOTCH3 RECEPTOR; PROTEIN; PROTEIN BCL 2; PROTEIN P63; RAC1 PROTEIN; RNA;

ARTICLE; AUTOMATION; COLORECTAL CARCINOMA; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENETIC ANALYSIS; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MELANOMA; MOLECULAR IMAGING; MULTI EPITOPE LIGAND CARTOGRAPHY; PERSONALIZED MEDICINE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; TISSUE SECTION;

MELC; QRT-PCR;

ANTIBODIES; COLORECTAL NEOPLASMS; EPITOPE MAPPING; FORMALDEHYDE; GENE EXPRESSION PROFILING; HUMANS; MELANOMA; PARAFFIN EMBEDDING; PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; TISSUE FIXATION;

EID: 84877329596     PISSN: 00144800     EISSN: 10960945     Source Type: Journal    
DOI: 10.1016/j.yexmp.2013.03.008     Document Type: Article

References (11)

1. Bohmann K., Hennig G., Rogel U., Poremba C., Mueller B.M., Fritz P., Stoerkel S., Schaefer K.L. RNA extraction from archival formalin-fixed paraffin-embedded tissue: a comparison of manual, semiautomated, and fully automated purification methods. Clinical Chemistry 2009, 55(9):1719-1727.
2. Bonnekoh B., Böckelmann R., Pommer A.J., Malykh Y., Philipsen L., Gollnick H. The CD11a binding site of efalizumab in psoriatic skin tissue as analysed by multi epitope ligand cartography (MELC) robot technology: introduction of a novel biological drug binding biochip assay. Skin Pharmacology and Physiology 2007, 20:96-111.
3. Camp R., Chung G., Rimm D. Automated subcellular localization and quantification of protein expression in tissue microarrays. Nature Medicine 2002, 8(11):1323-1327.
4. Eyerich K., Böckelmann R., Pommer A.J., Foerster S., Hofmeister H., Huss-Marp J., Cavani A., Behrendt H., Ring J., Gollnick H., Bonnekoh B., Traidl-Hoffmann C. Comparative in situ topoproteome analysis reveals differences in patch test-induced eczema: cytotoxicity dominated nickel versus pleiotrope pollen reaction. Experimental Dermatology 2010, 19:511-517.
5. Ghazalpour A., Bennett B., Petyuk V.A., Orozco L., Hagopian R., Mungrue I.N., Farber C.R., Sinsheimer J., Kang H.M., Furlotte N., Park C.C., Wen P.Z., Brewer H., Weitz K., Camp D.G., Pan C., Yordanova R., Neuhaus I., Tilford C., Siemers N., Gargalovic P., Eskin E., Kirchgessner T., Smith D.J., Smith R.D., Lusis A.J. Comparative analysis of proteome and transcriptome variation in mouse. PLoS Genetics 2011, 7(6):e1001393.
6. Gygi S.P., Rochon Y., Franza B.R., Aebersold R. Correlation between protein and mRNA abundance in yeast. Molecular and Cellular Biology 1999, 19(3):1720-1730.
7. Levenson R., Fornari A., Lode M. Multispectral imaging and pathology: seeing and doing more. Expert Opinion Medicine Diagnostic 2008, 2(9):1067-1081.
8. Müller B.M., Kronenwett R., Hennig G., Euting H., Weber K., Bohmann K., Weichert W., Altmann G., Roth C., Winzer K.J., Kristiansen G., Petry C., Dietel M., Denkert C. Quantitative determination of estrogen receptor, progesterone receptor, and HER2 mRNA in formalin-fixed paraffin-embedded tissue - a new option for predictive biomarker assessment in breast cancer. Diagnostic Molecular Pathology 2011, 20(1):1-10.
9. Petry C., Gehrmann M., von Torne C., Weber K., Stropp U., Hennig G. Predictive and prognostic markers of breast cancer. Molecular biological analysis of fixed tumor tissue. Pathologe 2008, 29(Suppl. 2):181-183.
10. Schubert W., Bonnekoh B., Pommer A.J., Philipsen L., Böckelmann R., Malykh Y., Gollnick H., Friedenberger M., Bode M., Dress A.W.M. Analyzing proteome topology and function by automated multidimensional fluorescence microscopy. Nature Biotechnology 2006, 24(10):1270-1278.
11. Wolf M.J., Hoos A., Bauer J., Boettcher S., Knust M., Weber A., Simonavicius N., Schneider C., Lang M., Stürzl M., Croner R.S., Konrad A., Manz M.G., Moch H., Aguzzi A., van Loo G., Pasparakis M., Prinz M., Borsig L., Heikenwälder M. Endothelial CCR2 signaling induced by colon carcinoma cells enables extravasation via the JAK2-Stat5 and p38MAPK pathway. Cancer Cell 2012, 22(1):91-105.