Recombineering to homogeneity: Extension of multiplex recombineering to large-scale genome editing

Biotechnology Journal

Volumn 8, Issue 5, 2013, Pages 515-522

  Boyle, Nanette R ^a   Reynolds, T Steele ^a   Evans, Ron ^b   Lynch, Michael ^b   Gill, Ryan T ^a  

^a UCB 450   (United States)

^b OPX Biotechnologies Inc   (United States)

Author keywords

Genome engineering; Lambda Red; Multiple chromosomes; Rec E T; Recombineering efficiency

Indexed keywords

GENOME ENGINEERING; LAMBDA RED; MULTIPLE CHROMOSOMES; REC E/T; RECOMBINEERING;

ESCHERICHIA COLI; GENES; GENETIC ENGINEERING; MULTIPLEXING;

EFFICIENCY;

DOUBLE STRANDED DNA; OKAZAKI FRAGMENT;

AUTOMATION; BACTERIOPHAGE; CALCULATION; CHROMOSOME; DNA REPLICATION; ESCHERICHIA COLI; GENE REPLICATION; GENE TARGETING; GENETIC ENGINEERING; GENETIC RECOMBINATION; LARGE SCALE GENOME EDITING; LARGE SCALE PRODUCTION; NONHUMAN; PRIORITY JOURNAL; RECOMBINEERING; REVIEW; RNA EDITING;

BIOTECHNOLOGY; DNA, RECOMBINANT; GENETIC ENGINEERING; GENETIC VECTORS; GENOMICS; RECOMBINATION, GENETIC;

EID: 84877158197     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201200237     Document Type: Review

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