Testing Insecticidal Activity of Novel Chemically Synthesized siRNA against Plutella xylostella under Laboratory and Field Conditions

PLoS ONE

Volumn 8, Issue 5, 2013, Pages

  Gong, Liang ^a   Chen, Yong ^a   Hu, Zhen ^a   Hu, Meiying ^a  

^a SOUTH CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA; SMALL INTERFERING RNA;

ACETYLCHOLINESTERASE 1 GENE; ACETYLCHOLINESTERASE 2 GENE; ARTICLE; BIOASSAY; BIOSAFETY; BRASSICA; BRASSICA ALBOGLABRA; BRASSICA OLERACEA; CHEMICAL PEST CONTROL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; GENE EXPRESSION; INSECTICIDAL ACTIVITY; LC 50; LETHALITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLUTELLA XYLOSTELLA; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; TOXICITY TESTING; TRANSCRIPTION TERMINATION;

ACETYLCHOLINESTERASE; ANIMALS; BIOLOGICAL ASSAY; BRASSICA; CHEMISTRY TECHNIQUES, SYNTHETIC; GENE EXPRESSION REGULATION, ENZYMOLOGIC; LABORATORIES; MOTHS; PEST CONTROL, BIOLOGICAL; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING;

EID: 84877135218     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0062990     Document Type: Article

References (62)

1. Taylor P, Radic Z, (1994) the cholinesterases: from genes to proteins. Annu Rev Pharmacol Toxicol 34: 281-320.
2. Kakani EG, Trakala M, Drosopoulou E, Mavragani-Tsipidou P, Mathiopoulos KD (2012) Genomic structure, organization and localization of the acetylcholinesterase locus of the olive fruit fly, Bactrocera oleae. Bull Entomol Res. doi:10.1017/S0007485312000478.
3. Zhou LJ, Huang JG, Xu HH, (2011) Monitoring resistance of field populations of diamondback moth Plutella xylostella L. (Lepidoptera: Yponomeutidae) to five insecticides in South China: A ten-year case study. Crop Prot. 30: 272-278.
4. Pang YP, Singh SK, Gao Y, Lassiter TL, Mishra RK, et al. (2009) Selective and irreversible inhibitors of aphid acetylcholinesterases: steps toward human-safe insecticides. PLoS ONE 4: e4349.
5. Talekar NS, Shelton AM, (1993) Biology, ecology, and management of the diamondback moth. Annu Rev Entomol. 38: 275-301.
6. Furlong MJ, Wright DJ, Dosdall LM, (2013) Diamondback Moth Ecology and Management: Problems, Progress and Prospects. Annu Rev Entomol. 58: 517-541.
7. Grzywacz D, Rossbach A, Rauf A, Russell D, Srinivasan R, et al. (2010) Current control methods for diamondback moth and prospects for improved management with lepidopteran-resistant Bt vegetable brassicas in Asia and Africa. Crop Protect. 29: 68-79.
8. Endersby NM, Viduka K, Baxter SW, Saw J, Heckel DG,et al. 2011. Widespread pyrethroid resistance in Australian diamondback moth, Plutella xylostella (L.), is related to multiple mutations in the para sodium channel gene. Bull Entomol Res. 101: 393 cha.
9. Lu Y, Pang Y-P, Park Y, Gao X, Yao J, et al. (2012) Genome Organization, Phylogenies, Expression Patterns, and Three-Dimensional Protein Models of Two Acetylcholinesterase Genes from the Red Flour Beetle. PLoS ONE 7(2): e32288 doi:10.1371/journal.pone.0032288.
10. Liu XX, Chen M, Collins HL, Onstad D, Roush R, et al. (2012) Effect of Insecticides and Plutella xylostella (Lepidoptera: Plutellidae) Genotype on a Predator and Parasitoid and Implications for the Evolution of Insecticide Resistance. J Econ Entomol. 105(2): 354-362.
11. Zhao JZ, Collins HL, Li XY, Mau RFL, Thompson GD, et al. (2006) Monitoring of diamondback moth resistance to spinosad, indoxacarb and emamectin benzoate. J Econ Entomol. 99: 176-181.
12. Santos VC, Siqueira HA, Silva JE, Farias MJ, (2011) Insecticide resistance in populations of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), from the state of Pernambuco, Brazil. Neotrop Entomol. 40(2): 264-270.
13. Fire AZ, (2007) Gene silencing by double-stranded RNA (Nobel lecture). Angew Chem Int Ed Engl.46 (37): 6967-6984.
14. Whang JS, Hunter CP, (2008) Environmental RNA interference. Trends Genet 24(6): 297-305.
15. Jinek M, Doudna JA, (2009) A three-dimensional view of the molecular machinery of RNA interference. Nature. 457(7228): 405-412.
16. Kumar M. Gupta GP, Rajam MV, (2009) Silencing of acetylcholinesterase gene of Helicoverpa armigera by siRNA affects larval growth and its life cycle. J Insect Physiol 55(3): 273-278.
17. Gong L, Yang XQ, Zhang BL, Zhong GH, Hu MY, (2011) Silencing of Rieske iron-sulfur protein using chemicallyly synthesised siRNA as a potential biopesticide against Plutella xylostella. Pest Manag Sci. 67(5): 514-520.
18. Helle W, Overmeer WPJ (1985) Toxicological methods. 183-188.
19. Teng XL, Zhang Z, He GL, Yang LW, Li F, (2012) Validation of reference genes for quantitative expression analysis by real-time RT-PCR in four lepidopteran insects. J Insect Sci. 12: 60.
20. Livak KJ, Schmittgen TD, (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods. 25: 402-408.
21. Ellman GL, Courtney KD, Andres JV, Featherstone RM, (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 7: 88-96.
22. Hsu JC, Wu WJ, Haymerc DS, Liao HY, Feng HT, (2008) Alterations of the acetylcholinesterase enzyme in the oriental fruit fly Bactrocera dorsalis are correlated with resistance to the organophosphate insecticide fenitrothion. Insect Biochem Mol Bio 38: 146-154.
23. Abbott WS, (1925) A method of computing the effectiveness of an insecticide. J Econo Entom 18: 256-257.
24. Huvenne H, Smagghe G, (2010) Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review. J Insect Physiol. 56: 227-235.
25. Li X, Zhang M, Zhang H, (2011) RNA Interference of Four Genes in Adult Bactrocera dorsalis by Feeding Their dsRNAs. PLoS ONE 6(3): e17788.
26. Whangbo J, Hunter C, (2008) Environmental RNA interference. Trends Genet. 24: 297-305.
27. Zhu J-Q, Liu S, Ma Y, Zhang J-Q, Qi H-S, et al. (2012) Improvement of Pest Resistance in Transgenic Tobacco Plants Expressing dsRNA of an Insect-Associated Gene EcR. PLoS ONE 7(6): e38572.
28. Zhang P, Cao G, Sheng J, Xue R, Gong C, (2012) BmTGIF, a Bombyx mori Homolog of Drosophila DmTGIF, Regulates Progression of Spermatogenesis. PLoS ONE 7(11): e47861.
29. Burand JP, Hunter WB (2012) RNAi: Future in insect management. J Invertebr Pathol. Available: http://dx.doi.org/10.1016/j.jip.2012.07.012.
30. Bolognesi R, Ramaseshadri P, Anderson J, Bachman P, Clinton W, et al. (2012) Characterizing the Mechanism of Action of Double-Stranded RNA Activity against Western Corn Rootworm (Diabrotica virgifera virgifera LeConte). PLoS ONE 7(10): e47534.
31. Terenius O, Papanicolaou A, Garbutt JS, Eleftherianos I, Huvenne H, et al. (2011) RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design. J Insect Physiol. 57: 231-245.
32. Mao J, Zeng F, (2012) Feeding-Based RNA Intereference of a Gap Gene Is Lethal to the Pea Aphid, Acyrthosiphon pisum. PLoS ONE 7(11): e48718.
33. Gong L, Luo Q, Rizwan-ul-Haq M, Hu MY, (2012) Cloning and characterization of three chemosensory proteins from Spodoptera exigua and effects of gene silencing on female survival and reproduction. Bull Entomol Res. 102: 600-609.
34. Wuriyanghan H, Rosa C, Falk B, (2011) Oral delivery of double-stranded RNAs and siRNAs induces RNAi effects in the potato/tomato psyllid, Bactericerca cockerelli. PLoS ONE. 6: e27736.
35. Chandler D, Bailey AS, Tatchell GM, Davidson G, Greaves J, et al. (2011) The development, regulation and use of biopesticides for integrated pest management. Phil Trans R Soc. B. 366: 1987-1998.
36. Silva AX, Jander G, Samaniego H, Ramsey JS, Figueroa CC, (2012) Insecticide Resistance Mechanisms in the Green Peach Aphid Myzus persicae (Hemiptera: Aphididae) I: A Transcriptomic Survey. PLoS ONE 7(6): e36366.
37. Boller EF, Vogt H, Ternes P, Malavolta C (2006) Working Document on Selectivity of Pesticides (2005). Internal newsletter issued by the publication commission for the IOBC/wrps council and executive committee issue no.40.
38. Revuelta L, Piulachs MD, Bellés X, Castañera P, Ortego F, et al. (2009) RNAi of ace1 and ace2 in Blattella germanica reveals their differential contribution to acetylcholinesterase activity and sensitivity to insecticides. Insect Biochem Mol Biol. 39: 913-919.
39. Hui XM, Yang LW, He GL, Yang QP, Han ZJ, et al. (2011) RNA interference of ace1 and ace2 in Chilo suppressalis reveals their different contributions to motor ability and larval growth. Insect Mol Biol. 20: 507-518.
40. Lu YH, Park YS, Gao XW, Zhang X, Yao JX, et al. (2012) Cholinergic and non-cholinergic functions of two acetylcholinesterase genes revealed by gene-silencing in Tribolium castaneum. Sci Rep. 2: 288.
41. Tomoyasu Y, Denell RE, (2004) Larval RNAi in Tribolium (Coleoptera) for analyzing adult development. Dev Genes Evol. 214: 575-578.
42. Tomoyasu Y, Miller SC, Tomita S, Schoppmeier M, Grossmann D, et al. (2008) Exploring systemic RNA interference in insects: a genome wide survey for RNAi genes in Tribolium. Genome Biol. 9: R10.
43. Lee DW, Kim SS, Shin SW, Kim WT, Boo KS, (2006) Molecular characterization of two acetylcholinesterase genes from the oriental tobacco budworm, Helicoverpa assulta (Guenee). Biochim Biophys Acta. 1760(2): 125-133.
44. Lee DW, Choi JY, Kim WT, Je YH, Song JT, et al. (2007) Mutations of acetylcholinesterase1 contribute to prothiofos-resistance in Plutella xylostella (L.). Biochem Biophys Res Commun. 353(3): 591-597.
45. Jiang X, Qu M, Denholm I, Fang J, Jiang W, et al. (2009) Mutation in acetylcholinesterase1 associated with triazophos resistance in rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae). Biochem Biophys Res Commun. 378(2): 269-272.
46. Weill M, Fort P, Berthomieu A, Dubois MP, Pasteur N, et al. (2002) A novel acetylcholinesterase gene in mosquitoes codes for the insecticide target and is non-homologous to the ace gene in Drosophila. Proc Biol Sci 269(1504): 2007-2016.
47. Chen Z, Newcomb R, Forbes E, McKenzie J, Batterham P, (2001) The acetylcholinesterase gene and organophosphorus resistance in the Australian sheep blowfly, Lucilia cuprina. Insect Biochem Mol Biol. 31(8): 805-816.
48. Temeyer KB, Chen AC, (2007) Identification and characterization of a cDNA encoding th acetylcholinesterase of Haematobia irritans (L.) (Diptera: Muscidae). DNA Seq. 18(2): 85-91.
49. Khalaf SA, (2004) Production and characterization of fungal chitosan under solid-state fermentation conditions. Int J Agri Biol. 6(6): 1033-1036.
50. Linden JC, Stoner RJ. Knutson KW, Gardner-Hughes CA, (2000) organic disease control elicitors. Agro Food Industry Hi-Tech. 11(5): 32-34.
51. Kao CH, Hsiang CY, Ho TY, (2012) Assessment of Chitosan-Affected Metabolic Response by Peroxisome Proliferator-Activated Receptor Bioluminescent Imaging-Guided Transcriptomic Analysis. PLoS ONE 7(4): e34969.
52. Liu H, Du Y, Wang X, Sun L, (2004) Chitosan kills bacteria through cell membrane damage. Intl J Food Microbiol. 95(2): 147-155.
53. Zhang X, Zhang J, Zhu KY, (2010) Chitosan/double-stranded RNA nanoparticle-mediated RNA interference to silence chitin synthase genes through larval feeding in the African malaria mosquito (Anopheles gambiae). Insect Mol Biol. 19(5): 683-693.
54. Gary DJ, Puri N, (2007) Won YY, (2007) Polymer-based siRNA delivery: Perspectives on the fundamental and phenomenological distinctions from polymer-based DNA delivery. J Control Rel. 121: 64-73.
55. Huang Z, King MR, (2009) An immobilized nanoparticle-based platform for efficient gene knockdown of targeted cells in the circulation. Gene Therapy. 16: 1271-1282.
56. Rabea EI, Badawy ME, Rogge TM, Stevens CV, Hofte M, et al. (2005) Insecticidal and fungicidal activity of new synthesized chitosan derivatives. Pest Manag Sci. 61: 951-960.
57. Badawy MEI, El-Aswad AF, (2012) Insecticidal activity of chitosans of different molecular weights and chitosan-metal complexes against cotton leafworm Spodoptera littoralis and oleander aphid Aphis nerii. Plant Protect Sci. 48: 131-141.
58. Zhang M, Tan TW, Yuan HZ, Rui CH, (2003) Insecticidal and Fungicidal Activities of Chitosan and Oligo-chitosan. J Bioact Compat Pol. 18: 391-400.
59. Thacker JRM (2002) An introduction to arthropod pest control. Cambridge, UK: Cambridge University Press.
60. Boese QF, Scaringe SA, Marshall WS, (2003) siRNA as a tool for streamlining functional genomic studies. Targets. 2(3): 93-99.
61. Lasota JA, Dybas RA, (1991) Avermectins, a novel class of compounds: implications for use in arthropod pest control. Annu Rev Entomol. 36: 91-117.
62. Marrone PG, (1999) Microbial pesticides and natural products as alternatives. Outlook Agri. 28: 149-149.