The development of standard samples with a defined number of antigen-specific T cells to harmonize T cell assays: A proof-of-principle study

Cancer Immunology, Immunotherapy

Volumn 62, Issue 3, 2013, Pages 489-501

  Singh, Satwinder Kaur ^a   Tummers, Bart ^a   Schumacher, Ton N ^b   Gomez, Raquel ^b   Franken, Kees L M C ^a   Verdegaal, Els M ^a   Laske, Karoline ^c   Gouttefangeas, Cécile ^c   Ottensmeier, Christian ^d   Welters, Marij J P ^a   Britten, Cedrik M ^e   Van Der Burg, Sjoerd H ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b NETHERLANDS CANCER INSTITUTE   (Netherlands)

^c UNIVERSITY OF TÜBINGEN   (Germany)

^d UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^e JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Assay controls; HLA peptide multimer staining; Immunomonitoring; Immunotherapy; T cells

Indexed keywords

HLA A ANTIGEN; LIQUID NITROGEN; TETRAMER; HLA A*02:01 ANTIGEN; HLA A2 ANTIGEN; HLA ANTIGEN; HLA-A*02:01 ANTIGEN; LYMPHOCYTE ANTIGEN RECEPTOR; TUMOR ANTIGEN;

ACCURACY; ANTIGEN SPECIFIC T CELL; ANTIGEN SPECIFICITY; ARTICLE; CD3+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CELL ASSAY; CELL SELECTION; CONTROLLED STUDY; CRYOPRESERVATION; IMMUNE RESPONSE; IN VITRO STUDY; LYMPHOCYTE SUBPOPULATION; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PURIFICATION; QUALITY CONTROL; T LYMPHOCYTE; BIOASSAY; EVALUATION; GENETIC TRANSDUCTION; HUMAN; IMMUNOLOGICAL MONITORING; IMMUNOLOGY; METHODOLOGY; MONONUCLEAR CELL; OBSERVER VARIATION; REFERENCE VALUE; STAINING; STANDARD; TRANSGENE;

ANTIGENS, NEOPLASM; BIOLOGICAL ASSAY; CD8-POSITIVE T-LYMPHOCYTES; HLA ANTIGENS; HLA-A2 ANTIGEN; HUMANS; LEUKOCYTES, MONONUCLEAR; MONITORING, IMMUNOLOGIC; OBSERVER VARIATION; RECEPTORS, ANTIGEN, T-CELL; REFERENCE VALUES; STAINING AND LABELING; TRANSDUCTION, GENETIC; TRANSGENES;

MLCS; MLOWN;

EID: 84877130799     PISSN: 03407004     EISSN: 14320851     Source Type: Journal    
DOI: 10.1007/s00262-012-1351-0     Document Type: Article

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