Xeno-free adaptation and culture of human pluripotent stem cells

Methods in Molecular Biology

Volumn 1001, Issue , 2013, Pages 81-97

  Sampsell Barron, Tori ^a  

^a Life Technologies   (United States)

Author keywords

Human embryonic stem cell; Human pluripotent stem cell; Induced pluripotent stem cell; Xeno free

Indexed keywords

ARTICLE; ASPIRATION; BIOSAFETY; CELL CULTURE; CELL DIFFERENTIATION; CELL LOSS; CELL STRUCTURE; CRYOPRESERVATION; CULTURE MEDIUM; CULTURE VESSEL; DRUG SCREENING; EMBRYONIC STEM CELL; FLOW CYTOMETRY; GENETIC STABILITY; HUMAN; HUMAN CELL; HUMAN DEVELOPMENT; IMMUNOREACTIVITY; INCUBATION TIME; KARYOTYPE; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; REPRODUCIBILITY; ROOM TEMPERATURE; THAWING; TISSUE CULTURE; TRANSFER CELL; XENOGRAFT;

CELL CULTURE TECHNIQUES; CULTURE MEDIA; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; HUMANS; KARYOTYPING; PLURIPOTENT STEM CELLS; XENOBIOTICS;

ANIMALIA; IPS;

EID: 84877010318     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-363-3_8     Document Type: Article

References (15)

1. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al (1998) Embryonic stem cell lines derived from human blastocysts. Science 282:1145-1147
2. Yu J, Vodyanik MA, Smuga-Otto K et al (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318:1917-1920
3. Takahashi K, Tanabe K, Ohnuki M et al (2007) Induction of pluripotent stem cells from adult human fi broblasts by de fi ned factors. Cell 131:861-872
4. Akopian V, Andrews PW, Beil S et al (2010) Comparison of de fi ned culture systems for feeder cell free propagation of human embryonic stem cells. In Vitro Cell Dev Biol Anim 46:247-258
5. Mallon B, Park KY, Chen KG et al (2006) Toward xeno-free culture of human embryonic stem cells. Int J Biochem Cell Biol 38:1063-1075
6. Xu C, Inokuma MS, Denham J et al (2001) Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol 19:971-974
7. Amit M, Shariki C, Margulets V et al (2004) Feeder layer- and serum-free culture of human embryonic stem cells. Biol Reprod 70:837-845
8. Ludwig TE, Bergendahl V, Levenstein ME et al (2006) Feeder-independent culture of human embryonic stem cells. Nat Methods 3:637-646
9. Wagner KE, Vemuri MC (2010) Serum-free and feeder-free culture expansion of human embryonic stem cells. Methods Mol Biol 584:109-119
10. Vemuri MC, Schimmel T, Colls P et al (2007) Derivation of human embryonic stem cells in xeno-free condition. In: Vemuri MC (ed) Stem cell assays. Humana, Totowa, NJ, pp 1-10
11. Chen G, Gulbranson DR, Hou Z et al (2011) Chemically de fi ned conditions for human iPSC derivation and culture. Nat Methods 8:424-429
12. Rajala K, Vaajasaari H, Suuronen R et al (2011) Effects of the physiochemical culture environment on the stemness and pluripotency of human embryonic stem cells. Stem Cell Stud 1:e3. doi: 10.4081/scs.2011.e3
13. Watanabe K, Ueno M, Kamiya D et al (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 25:681-686
14. http://www.jove.com/video/1427/cultureand- maintenance-of-human- embryonic-stemcells
15. Xu Y, Zhu X, Hahm HS et al (2010) Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules. Proc Natl Acad Sci USA 107:8129-8134