Standard dyes for total protein staining in gel-based proteomic analysis

Materials

Volumn 3, Issue 10, 2010, Pages 4784-4792

  Chevalier, François ^a  

^a INSTITUT DE RADIOPROTECTION ET DE SÛRETÉ NUCLÉAIRE   (France)

Author keywords

Fluorescent dyes; Protein staining; Proteomics; Two dimensional electrophoresis

Indexed keywords

FLUORESCENT DYES; PROTEOMIC ANALYSIS; PROTEOMIC STUDIES; PROTEOMICS; REPRODUCIBILITIES; TWO DIMENSIONAL ELECTROPHORESIS; TWO-DIMENSIONAL GEL ELECTROPHORESIS; TWO-DIMENSIONAL GELS;

ELECTROPHORESIS; FLUORESCENCE; MOLECULAR BIOLOGY; TWO DIMENSIONAL;

PROTEINS;

EID: 84876861307     PISSN: None     EISSN: 19961944     Source Type: Journal    
DOI: 10.3390/ma3104784     Document Type: Review

References (23)

1. Chevalier, F. Highlights on the capacities of "Gel-based" proteomics. Proteome Sci. 2010, 8, 23.
2. Patton, W.F. Detection technologies in proteome analysis. J. Chromatogr. B 2002, 771, 3-31.
3. Diezel, W.; Kopperschläger, G.; Hofmann, E. An improved procedure for protein staining in polyacrylamide gels with a new type of Coomassie Brilliant Blue. Anal. Biochem. 1972, 48, 617-620.
4. Neuhoff, V.; Arold, N.; Taube, D.; Ehrhardt, W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 1988, 9, 255-262.
5. Mortz, E.; Krogh, T.N.; Vorum, H.; Gorg, A. Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis. Proteomics 2001, 1, 1359-1363.
6. Westermeier, R.; Marouga, R. Protein detection methods in proteomics research. Bioscience Rep. 2005, 25, 19-32.
7. Berggren, K.; Chernokalskaya, E.; Steinberg, T.H.; Kemper, C.; Lopez, M.F.; Diwu, Z.; Haugland, R.P.; Patton, W.F. Background-free, high sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels using a luminescent ruthenium complex. Electrophoresis 2000, 21, 2509-2521.
8. Rabilloud, T.; Strub, J.M.; Luche, S.; Van Dorsselaer, A.; Lunardi, J. Comparison between Sypro Ruby and ruthenium II tris (bathophenanthroline disulfonate) as fluorescent stains for protein detection in gels. Proteomics 2001, 1, 699-704.
9. Mackintosh, J.A.; Choi, H.Y.; Bae, S.H.; Veal, D.A.; Bell, P.J.; Ferrari, B.C.; Van Dyk, D.D.; Verrills, N.M.; Paik, Y.K.; Karuso, P. A fluorescent natural product for ultra sensitive detection of proteins in one-dimensional and two-dimensional gel electrophoresis. Proteomics 2003, 3, 2273-2288.
10. Viswanathan, S.; Unlu, M.; Minden, J.S. Two-dimensional difference gel electrophoresis. Nat. Protocol. 2006, 1, 1351-1358.
11. Chevalier, F.; Rofidal, V.; Rossignol, M. Visible and fluorescent staining of two-dimensional gels. In Plant Proteomics: Methods in Molecular Biology; Thiellement, H., Zivy, M., Damerval, C., Méchin, V., Eds.; Humana Press Inc.: Totowa, NJ, USA, 2007; vol. 335, pp. 145-156.
12. Scheler, C.; Lamer, S.; Pan, Z.; Li, X.P.; Salnikow, J.; Jungblut, P. Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Electrophoresis 1998, 19, 918-927.
13. Chevalier, F.; Rofidal, V.; Vanova, P.; Bergoin, A.; Rossignol, M. Proteomic capacity of recent fluorescent dyes for protein staining. Phytochemistry 2004, 65, 1499-1506.
14. Chevalier, F.; Martin, O.; Rofidal, V.; Devauchelle, A.D.; Barteau, S.; Sommerer, N.; Rossignol, M. Proteomic investigation of natural variation between Arabidopsis ecotypes. Proteomics 2004, 4, 1372-1381.
15. Chevalier, F.; Centeno, D.; Rofidal, V.; Tauzin, M.; Martin, O.; Sommerer, N.; Rossignol, M. Different impact of staining procedures using visible stains and fluorescent dyes for large-scale investigation of proteomes by MALDI-TOF mass spectrometry. J. Proteome Res. 2006, 5, 512-520.
16. Rabilloud, T. A comparison between low background silver diammine and silver-nitrate protein stains. Electrophoresis 1992, 13, 429-439.
17. Richert, S.; Luche, S.; Chevallet, M.; Van Dorsselaer, A.; Leize-Wagner, E.; Rabilloud, T. About the mechanism of interference of silver staining with peptide mass spectrometry. Proteomics 2004, 4, 909-916.
18. Berggren, K.N.; Schulenberg, B.; Lopez, M.F.; Steinberg, T.H.; Bogdanova, A.; Smejkal, G.; Wang, A.; Patton, W.F. An improved formulation of SYPRO Ruby protein gel stain: Comparison with the original formulation and with a ruthenium II tris (bathophenanthroline disulfonate) formulation. Proteomics 2002, 2, 486-498.
19. Berggren, K.; Steinberg, T.H.; Lauber, W.M.; Carroll, J.A.; Lopez, M.F.; Chernokalskaya, E.; Zieske, L.; Diwu, Z.J.; Haugland, R.P.; Patton, W.F. A luminescent ruthenium complex for ultrasensitive detection of proteins immobilized on membrane supports. Anal. Biochem. 1999, 276, 129-143.
20. Bell, P.J.L.; Karuso, P. Epicocconone, a novel fluorescent compound from the fungus Epicoccum nigrum. J. Am. Chem. Soc. 2003, 125, 9304-9305.
21. Patton, W.F. Detection technologies in proteome analysis. J. Chromatogr. B 2002, 771, 3-31.
22. Hrebicek, T.; Duerrschmid, K.; Auer, N.; Bayer, K.; Rizzi, A. Effect of CyDye minimum labeling in differential gel electrophoresis on the reliability of protein identification. Electrophoresis 2007, 28, 1161-1169.
23. Shaw, J.; Rowlinson, R.; Nickson, J.; Stone, T.; Sweet, A.; Williams, K.; Tonge, R. Evaluation of saturation labelling two-dimensional difference gel electrophoresis fluorescent dyes. Proteomics 2003, 3, 1181-1195.