Phenotypic and genotypic analysis of virulence in Staphylococcus spp. And its clonal dispersion as a contribution to the study of bovine mastitis;Análise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina

Pesquisa Veterinaria Brasileira

Volumn 33, Issue 2, 2013, Pages 161-170

  Marques, Viviane F ^a   de Souza, Miliane M S ^a   de Mendonça, Elaine C L ^a   de Alencar, Tatiani Abreu ^a,b   Pribul, Bruno Rocha ^a   de M de O Coelho, Shana ^a   Lasagno, Mirta ^b   Reinoso, Elina B ^b  

^a FEDERAL RURAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b DEPARTAMENTO DE QUÍMICA   (Argentina)

Author keywords

Mastitis; Staphylococcus spp.; Virulence factors

Indexed keywords

BACTERIA (MICROORGANISMS); BOVINAE; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS INTERMEDIUS;

EID: 84876738520     PISSN: 0100736X     EISSN: 16785150     Source Type: Journal    
DOI: 10.1590/S0100-736X2013000200005     Document Type: Article

References (57)

1. Aarestrup F.M., Dangler C.A. & Sordillo L.M. 1995. Prevalence of coagulase gene polymorphism in Staphylococcus aureus isolates causing bovine mastitis. Can. J. Vet. Res. 59:124-128.
2. Bannerman T.L. 2003. Staphylococcus, Micrococcus, and other catalase- -positive cocci that grow aerobically, p.384-404. In: Murray P.R., Baron E.J., Jorgensen J.H., Pfaller M.A. & Yolken R.H. (Eds), Manual of Clinical Microbiology. American Society Microbiology, Washington, DC.
3. Bannoehr J., Franco A., Iurescia M., Battisti A. & Fitzgerald J.R. 2009. Molecular Diagnostic Identification of Staphylococcus pseudintermedius. J. Clin. Microbiol. 47(2):469-471.
4. Cerca N., Jefferson K.K., Litrán T.M., Pier D.B., Quintos C.K., Goldman D.A., Azeredo J. & Pier G.B. 2007. Molecular basis for preferential protective efficacy of antibodies directed to the poorly acetylated form of staphylococcal poly-n-acetyl-_-(1-6)-glucosamine. Infect. Immun. 75(7):3406- 3413.
5. Chapman G., Berens C., Peters A. & Curcio L. 1934. Coagulase and hemolysin tests as measures of the pathogenicity of staphylococci. J. Bacteriol. 28(4):343-363.
6. Christensen G.D., Simpson W.A., Younger J.J., Baddour M.L., Barret F.F., Melton D.M. & Beachey E.H. 1985. Adherence of coagulase-active staphylococci to plastic tissue culture plates: A quantitative model for the adherence of staphylococci to medical devices. J. Clin. Microbiol. 22(6):996-1006.
7. Citak S., Varlik O. & Gundogan N. 2003. Slime production and DNase activity of Staphylococci isolated from raw milk. J. Food Safety 23:281-288.
8. Coelho S.M.S. 2008. Caracterizaçao fenotípica e genotípica de fatores de virulência e resistência à oxacilina em Staphylococcus spp. coagulase- positivos isolados de mastite bovina. Tese de doutorado em Ciências Veterinárias, Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, RJ. 77p.
9. Coelho S.M.O., Pereira I.A., Soares L.C., Pribul B.R. & Souza M.M.S. 2011. Short communication: Profile of virulence factors of Staphylococcus aureus isolated from subclinical bovine mastitis in the state of Rio de Janeiro, Brazil. J. Dairy Sci. 94(7):3305-3310.
10. Cruz R.S. 2008. Análise do efeito do lócus ag r sobre a formaçao de biofilme em cepas de Staphylococcus aureus. Tese de Doutorado em Ciências Biológicas (Microbiologia), Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, RJ. 96p.
11. Cucarella C., Solano C., Valle J., Amorena B., Lasa I.N.I. & Penade's J.R. 2001. Bap, a Staphylococcus aureus Surface Protein Involved in Biofilm Formation. J. Bacteriology. 183(9):2888-2896.
12. Daum R.S. & Spelberg B. 2011. Progress toward a Staphylococcus aureus vaccine. Clin. Infect. Dis. Advances. DOI: 10.1093/cid/cir828.
13. Dego K.O., Van Dijk J.E. & Nederbragt H. 2002. Factors involved in the early pathogenesis of bovine Staphylococcus aureus mastitis with emphasis on bacterial adhesion and invasion: A review. Vet. Microbiol. 24:181198.
14. Dendani Z., Arcangioli M.A., Bezille P., Ouzrout R. & Sellami N.L. 2010. Genotyping of Staphylococcus aureus isolated from bovine clinical mastitis by Pulsed-Field Gel Electrophoresis (PFGE). J. Anim. Vet. Advances 9(1):5-11.
15. Demo M. 1996. Caracterizacion y studios de patogenicidad de cepas dela genero Staphylococcus asiladas de leches mastiticas. Tese de Doutorado, Instituto de Microbiologia, Universidad Nacional de Rio Cuarto, Argentina.
16. Dobinsky S., Kiel K., Rohde H., Bartscht K., Knobloch J.K.M., Horstkotte M.A. & Mack D. 2003. Glucose-related dissociation between icaADBC transcription and biofilm expression by Staphylococcusepidermidis: Evidence for an additional factor required for polysaccharide intercellular adhesin synthesis. J. Bacteriol. 185(9):2879-2886.
17. El-Sayed A., Alber J., Lammer C., Jager S., Wolter W. & Vázquez H.C. 2006. Comparative study on genotypic properties of Staphylococcus aureus isolated from clinical and subclinical mastitis in Mexico. Vet. Méx. 37(2):165-179.
18. Ferreira L.M., Nader Filho A., Oliveira E., Zafalon L.F. & Souza V. 2006. Variabilidades fenotípica e genotípica de estirpes de Staphylococcus aureus isoladas em casos de mastite subclínica bovina. Ciência Rural 36(4):1228-1234.
19. Freitas M.F.L., Pinheiro Júnior J.W., Stamford T.L.M., Rabelo S.S.A., Silva D.R., Silveira Filho V.M., Santos F.G.B., Sena M.J. & Mota R.A. 2005. Perfil de sensibilidade antimicrobiana in vitro de Staphylococcus coagulase positivos isolados de leite de vacas com mastite no agreste do estado de Pernambuco. Arqs Inst. Biológico, Sao Paulo, 72(2):171-177.
20. Frenay H., Bunschoten A., Schouls L., Van Leeuwen W., Vandenbroucke-Grauls C., Verhoef J. & Mooi F. 1996. Molecular typing of methicillin-resistant Staphylococcus aureus on the basis of protein A gene polymorphism. Eur. J Clin. Microbiol. Infect. Dis. 15:60-64.
21. Gad G.F.M., El-Feky M.A., El-Rehewy M.S., Hassan M.A., Abolella H. & El-Baky R.M. 2009. Detection of icaA, icaD genes and biofilm production by Staphylococcus aureus and Staphylococcus epidermidis isolated from urinary tract catheterized patients. J. Infect. Dev. Countries 3(5):342- 351.
22. Gómez M., Seaghdha M.O. & Prince A.S. 2007. Staphylococcus aureus protein A activates TACE through EGFR-dependent signaling. EMBO Journal 26:701-709.
23. Greene C., McDevitt D., Francois P., Vaudaux P., Lew D.P. & Foster T.J. 1995. Adhesion properties of mutants of Stapbylococcus aureus defective in fibronectin-binding proteins and studies on expression of f n b genes. Mol. Microbiol. 17:1143-1152.
24. Handke L.D., Slater S.R., Conlon K.M., O'Donnell S.T., Olson M.E., Bryant K.A., Rupp M.E., O'Gara J.P. & Fey P.D. 2007. SigmaB and SarA independently regulate polysaccharide intercellular adhesin production in Staphylococcusepidermidis. Can. J. Microbiol. 53:82-91.
25. Karahan M. & Cetinkaya B. 2007. Coagulase gene polymorphisms detected by PCR in Staphylococcus aureus isolated from subclinical bovine mastitis in Turkey. Vet. Journal 174(2):428-431.
26. Koneman E.W., Allen S.D., Janda W.M, Schreckenberger P.C. & Winn Jr 2008. Diagnóstico Microbiológico. 6 a ed. Editora MEDS, Rio de Janeiro.
27. Krepsky N., Ferreira R.B.R., Nunes A.P.F., Lins U.G.C., Silva Filho F.C., Mattos Guaraldi A.L. & Netto-dos Santos K.R. 2003. Cell surface hydrophobicity and slime production of Staphylococcus epidermidis Brazilian isolates. Curr. Microbiol. 46:280-286.
28. Larsen H.D., Aarestup F.M. & Jensen N.E. 2002. Geographical variation in the presence of genes encoding superantigenic exotoxins and β-hemolysin among Staphylococcus aureus isolated from bovine mastitis in Europe and USA. Vet. Microbiol. 85:61-67.
29. Linehan D., Etienne J. & Sheehan D. 2003. Relationship between haemolytic and sphingomyelinase activities in a partially purified β-like toxin from Staphylococcus schleiferi. FEMS Immunol. Med. Microbiol. 3 6 (1 /2 ):9 5 -1 0 2.
30. Melchior M.B., Vaarkamp H. & Fink-Gremmels J. 2005. Biofilms: A role in recurrent mastitis infections?. Vet. Journal 171:398-407.
31. Nilsson I.-M., Hartford O., Foster T. & Tarkowski A. 1999. Alpha-toxin and gamma-toxin jointly promote Staphylococcus aureus virulence in murine septic arthritis. Infect. Immun. 67:1045-1049.
32. Novick R.P. & Jiang D. 2003. The staphylococcal saeRS system coordinates environmental signals with ag r quorum sensing. Microbiology 149:2709-2717.
33. O'Riordan K. & Lee J.C. 2004. Staphylococcus aureus capsular polysaccharides. Clin. Microbiol. Rev. 17(1):218-234.
34. Oliveira M., Bexiga R., Nunes S.F., Carneiro C., Cavaco L.M., Bernardo F. & Vilela C.L. 2006. Biofilm-forming ability profiling of Staphylococcus aureus and Staphylococcus epidermidis mastitis isolates. Vet. Microbiol. 118:133-140.
35. Ote I., Taminiau B., Duprez J.-N., Dizier I. & Mainil J.G. 2011. Genotypic characterization by polymerase chain reaction of Staphylococcus aureus isolates associated with bovine mastitis. Vet. Microbiol. 153: 285-292.
36. Park P.W., Foster T.J., Nishi E., Duncan S.J., Klagsbrun M. & Chen Y. 2004. Activation of syndecan-1 ectodomain shedding by Staphylococcus aureus a-toxin and β-toxin. J. Biol. Chem. 279(1):251-258.
37. Peacock S.J., Day N.P.J., Thomas M.G., Berendt A.R. & Foster T.J. 2000. Clinical isolates of Staphylococcus aureus exhibit diversity in fnb genes and adhesion to human fibronectin. J. Infection 41:23-31.
38. Pitkala A., Haveri M., Pyörälä S., Myllys V. & Honkanen-Buzalski T. 2004. Bovine mastitis in Finland 2001: prevalence, distribution of bacteria, and antimicrobial resistance. J. Dairy Sci. 87:2433-2441.
39. Pyorala S. & Taponen S. 2009. Coagulase-negative staphylococci: Emerging mastitis pathogens. Vet. Microbiol. 134:3-8.
40. Rabello R.F. 2007. Diversidade genética e genes de virulencia de amostras de Staphylococcus aureus isoladas de mastite bovina no Estado do Rio de Janeiro. Tese de Doutorado em Ciencias Biológicas (Microbiologia), Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, RJ. 161p.
41. Reinoso E.B. 2004. Análisis epidemiológico y molecuar de cepas de Staphylococcus aureus de distintos orígenes. Tese de Doutorado, Instituto de Microbiologia, Universidad Nacional de Rio Cuarto. 199p.
42. Reinoso E.B., El-Sayed A., Lammler C., Bognia C. & Zschockc M. 2008. Genotyping of Staphylococcus aureus isolated from humans, bovine subclinical mastitis and food samples in Argentina. Microbiol. Res. 163:314-322.
43. Sabersheikh S. & Saunders N.A. 2004. Quantification of virulence-associated gene transcripts in epidemic methicillin resistant Staphylococcus aureus by real-time PCR. Mol. Cell Probes 18(1):23-31.
44. Sambrook J., Fritsch E.F. & Maniatis T. 2002. Molecular Cloning: A laboratory manual. Cold Spring Harbor Lab. Press, New York.
45. Santos C.D.M. 2006. Staphylococcus sp e enterobactérias isoladas de mastite recorrente em oito rebanhos da regiao de Uberlândia/MG: perfil de suscetibilidade aos antimicrobianos. Mestrado em Produçao Animal, Faculdade de Medicina Veterinária, Universidade Federal de Uberlândia, MG. 69p.
46. Santos L.L. 2008. Staphylococcus coagulase negativo como agente de mamite em rebanhos bovinos leiteiros da regiao sul do Estado de Minas Gerais. Dissertaçao de Mestrado em Medicina Veterinària, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte. 40p.
47. Silva N. & Cardoso H.F.T. 2000. Produçao de toxinas hemolíticas por amostras de Staphylococcus aureus isoladas de mastite bovina. Vet. Notícias 6(2):63-67.
48. Silva E.C.B.F., Maciel M.A.V., Melo F.L., Antas M.G.C., Neto A.M.B. & Rabelo M.A. 2007. Staphylococcus aureus: aspectos biológicos e patogénicos. Anais Fac. Med. Univ. Fed. Pernambuco, Recife, 52(2):168-172.
49. Silva W.P., Silva A.J., Macedo M.R.P., Araújo M.R., Mata M.M. & Gandra E.A. 2003. Identification of Staphylococcus aureus, S. intermedius and S. hyicus by PCR amplification of coa and nuc genes. Braz. J. Microbiol. 34(Suppl. 1):125-127.
50. Straub J.A., Hertel C. & Hammes W.P. 1999. A 23S RNAr-targeted polymerase chain reaction-based system for detection of Staphylococcus aureus in meat started cultures and dairy products. J. Food Prot. 62:1150-1156.
51. Taponen S., Simojoki H., Haveri M., Larsen H.D. & Pyorala S. 2006. Clinical characteristics and persistence of bovine mastitis caused by different species of coagulase-negative staphylococci identified with API or AFLP. Vet. Microbiol. 115:199-207.
52. Tollersrud T., Kenny K., Reitz Jr A.J. & Lee J.C. 2000. Genetic and serologic evaluation of capsule production by bovine mammary isolates of Staphylococcus aureus and other Staphylococcus spp. from Europe and the United States. J. Clin. Microbiol. 38:2998-3003.
53. Vasudevan P., Nair M.K.M., Annamalai T. & Venkitanarayana K.S. 2003. Phenotypic and Genotipic characterization of bovine mastitis isolates of Staphylococcus aureus for biofilm formation. Vet. Microbiol. 92:179-185.
54. Watts J. 1988. Etiological agents of bovine mastitis. Vet. Microbiol. 16:41-46.
55. Zafalon L.F., Nader Filho A., Carvalho M.R.B. & Lima T.M.A. de. 2008. Influencia da mastite subclínica bovina sobre as fraçôes protéicas do leite. Arqs Inst. Biológico, Sao Paulo, 75(2):135-140.
56. Zhang K., Sparling J., Chow B.L., Elsayed S., Hussain Z., Church D.L., Gregson D.B., Louie T. & Conly J.M. 2004. New quadriplex PCR assay for detection of methicillin and mupirocin resistance and simultaneous discrimination of Staphylococcus aureus from coagulase-negative Staphylococci. J. Clin. Microbiol. 42(11):4947-4955.
57. Zhao L., Xue T., Shang F., Sun H. & Sun B. 2010. Staphylococcus aureus AI-2 quorum sensing associates with the KdpDE two-component system to regulate capsular polysaccharide synthesis and virulence. Infect. Immun. 78(8):3506-3515