An improved infectivity assay combining cell culture with real-time PCR for rapid quantification of human adenoviruses 41 and semi-quantification of human adenovirus in sewage

Water Research

Volumn 47, Issue 9, 2013, Pages 3183-3191

  Rodríguez, Roberto A ^a   Polston, Patsy M ^a   Wu, Ming Jing ^a   Wu, Jianyong ^a   Sobsey, Mark D ^a  

^a University of North Carolina at Chapel Hill   (United States)

Author keywords

CC mRNA; Detection; Enteric adenovirus; Real time PCR

Indexed keywords

ERROR DETECTION; POLYMERASE CHAIN REACTION; QUALITY ASSURANCE; SEWAGE; TRANSCRIPTION; VIRUSES;

CC/MRNA; CELL CULTURE METHODS; ENTERIC ADENOVIRUS; ENVIRONMENTAL SAMPLE; INITIAL CONCENTRATION; LINEAR RELATIONSHIPS; REAL-TIME PCR; REVERSE TRANSCRIPTION;

CELL CULTURE;

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MESSENGER RNA; VIRUS RNA;

BIOASSAY; CALIBRATION; CONCENTRATION (COMPOSITION); ESTIMATION METHOD; GENE; INFECTIOUS DISEASE; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME; RNA; SAMPLING; SEWAGE; VIRUS;

ARTICLE; CALIBRATION; CELL CULTURE; CONCENTRATION (PARAMETERS); CONTAMINATION; CONTROLLED STUDY; GENE EXPRESSION; HOUSEKEEPING GENE; HUMAN ADENOVIRUS; HUMAN ADENOVIRUS 41; HUMAN ADENOVIRUS INFECTION; INCUBATION TIME; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SEWAGE; VIRUS DETECTION; VIRUS INFECTIVITY;

ADENOVIRIDAE; HUMAN ADENOVIRUS; HUMAN ADENOVIRUS TYPE 41;

EID: 84876737729     PISSN: 00431354     EISSN: 18792448     Source Type: Journal    
DOI: 10.1016/j.watres.2013.03.022     Document Type: Article

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