Dengue virus: Isolation, propagation, quantification, and storage

Current Protocols in Microbiology

Volumn , Issue SUPPL.27, 2012, Pages

  Medina, Freddy ^a   Medina, Juan Francisco ^a   Colon, Candimar ^a   Vergne, Edgardo ^a   Santiago, Gilberto A ^a   Munoz Jordan, Jorge L ^a  

^a NATIONAL CENTER FOR EMERGING AND ZOONOTIC INFECTIOUS DISEASES   (United States)

Author keywords

Dengue; Flow cytometry; Immunofluorescence; Infection; Mosquito inoculation; Plaque assay; Titration

Indexed keywords

SUCROSE;

ARTICLE; CELL DIVISION; CELL GROWTH; CLINICAL PROTOCOL; DENGUE VIRUS; FLOW CYTOMETRY; IMMUNOFLUORESCENCE TEST; IN VIVO STUDY; INOCULATION; INSECT CELL; INVESTIGATIVE PROCEDURES; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SEROTYPING; THAWING; VERO CELL; VIRUS IDENTIFICATION; VIRUS ISOLATION; VIRUS PROPAGATION; VIRUS TITRATION; AEDES; ANIMAL; CELL LINE; CLASSIFICATION; DENGUE; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; PRESERVATION; VIROLOGY; VIRUS CULTURE;

AEDES; ANIMALS; CELL LINE; DENGUE; DENGUE VIRUS; HUMANS; PRESERVATION, BIOLOGICAL; VIROLOGY; VIRUS CULTIVATION;

EID: 84876701903     PISSN: 19348525     EISSN: 19348533     Source Type: Journal    
DOI: 10.1002/9780471729259.mc15d02s27     Document Type: Article

References (27)

1. Gubler,D.J. 1998. Dengue and dengue hemorrhagic fever. Clin. Microbiol. Rev. 11:480-496.
2. Gubler, D.J., Suharyono, W., Tan, R., Abidin, M., and Sie, A. 1981. Viraemia in patients with naturally acquired dengue infection. Bull. World Health Organ. 59:623-630.
3. Gubler, D.J., Kuno, G., Sather, G.E., Velez, M., and Oliver, A. 1984. Mosquito cell cultures and specific monoclonal antibodies in surveillance for dengue viruses. Am. J. Trop. Med. Hyg. 33:158-165.
4. Guzman, M.G. and Kouri, G. 2002. Dengue: an update. Lancet Infect Dis. 2:33-42.
5. Halstead, S.B. 1970. Observations related to pathogenesis of dengue hemorrhagic fever. VI. Hypotheses and discussion. Yale J. Biol. Med. 42:350-362.
6. Halstead, S.B. 1988. Pathogenesis of dengue: challenges to molecular biology. Science 239:476-481.
7. Holmes, E.C. and Twiddy, S.S. 2003. The origin, emergence and evolutionary genetics of dengue virus. Infect. Genet. Evol. 3:19-28.
8. Igarashi, A. 1978. Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. J. Gen. Virol. 40:531-544.
9. Kuno, G. and Oliver, A. 1989. Maintaining mosquito cell lines at high temperatures: effects on the replication of flaviviruses. In Vitro Cell Dev. Biol. 25:193-196.
10. Kuno, G., Gubler, D.J., Velez, M., and Oliver, A. 1985. Comparative sensitivity of three mosquito cell lines for isolation of dengue viruses. Bull. World Health Organ. 63:279-286.
11. Lambeth, C.R., White, L.J., Johnston, R.E., and de Silva, A.M. 2005. Flow cytometry-based assay for titrating dengue virus. J. Clin. Microbiol. 43:3267-3272.
12. Libraty, D.H., Young, P.R., Pickering, D., Endy, T.P., Kalayanarooj, S., Green, S., Vaughn, D.W., Nisalak, A., Ennis, F.A., and Rothman, A.L. 2002. High circulating levels of the dengue virus nonstructural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever. J. Infect. Dis. 186:1165-1168.
13. McElroy, K.L., Santiago, G.A., Lennon, N.J., Birren, B.W., Henn, M.R., and Munoz-Jord́an, J.L. 2011. Endurance, refuge, and reemergence of dengue virus type 2, Puerto Rico, 1986-2007. Emerg. Infect. Dis. 17:64-71.
14. Mukhopadhyay, S., Kuhn, R.J., and Rossmann, M.G. 2005. A structural perspective of the flavivirus life cycle. Nat. Rev. Microbiol. 3:13-22.
15. Ohainle, M., Balmaseda, A., Macalalad, A.R., Tellez, Y., Zody, M.C., Saboŕio, S., Nunez, A., Lennon, N.J., Birren, B.W., Gordon, A., Henn, M.R., and Harris, E. 2011. Dynamics of dengue disease severity determined by the interplay between viral genetics and serotype-specific immunity. Sci. Transl. Med. 3:114ra128.
16. Rico-Hesse, R. 2003. Microevolution and virulence of dengue viruses. Adv. Virus Res. 59:315-341.
17. Rico-Hesse, R., Harrison, L.M., Salas, R.A., Tovar, D., Nisalak, A., Ramos, C., Boshell, J., deMesa, M.T., Nogueira, R.M., and da Rosa, A.T. 1997. Origins of dengue type 2 viruses associated with increased pathogenicity in the Americas. Virology 230:244-251.
18. Rosen, L. 1981. The use of Toxorhynchites mosquitoes to detect and propagate dengue and other arboviruses. Am. J. Trop. Med. Hyg. 30:177-183.
19. Rosen, L. and Gubler, D. 1974. The use of mosquitoes to detect and propagate dengue viruses. Am. J. Trop. Med. Hyg. 23:1153-1160.
20. Rosen, L., Roseboom, L.E.,Gubler,D.J., Lien, J.C., and Chaniotis, B.N., 1985. Comparative susceptibility of mosquito species and strains to oral and parenteral infection with dengue and Japanese encephalitis viruses. Am. J. Trop. Med. Hyg. 34:603-615.
21. Sukhavachana, P., Nisalak, A., and Halstead, S.B. 1966. Tissue culture techniques for the study of dengue viruses. Bull. World Health Organ. 35:65-66.
22. Vaughn, D.W., Green, S., Kalayanarooj, S., Innis, B.L., Nimmannitya, S., Suntayakorn, S., Endy, T.P., Raengsakulrach, B., Rothman, A.L., Ennis, F.A., and Nisalak, A. 2000. Dengue viremia titer, antibody response pattern, and virus serotype correlate with disease severity. J. Infect. Dis. 181:2-9.
23. Vorndam, V. and Kuno, G. 1997. Laboratory diagnosis of dengue virus infections. In Dengue and Dengue Hemorrhagic Fever (D.J. Gubler, ed.) pp. 313-333. CAB International, London, United Kingdom.
24. Wang,W.K., Chao, D.Y., Kao, C.L.,Wu, H.C., Liu, Y.C., Li, C.M., Lin, S.C., Ho, S.T., Huang, J.H., and King, C.C. 2003. High levels of plasma dengue viral load during defervescence in patients with dengue hemorrhagic fever: Implications for pathogenesis. Virology 305:330-338.
25. Westaway, E.G. and Blok, J. 1997. Taxonomy and evolutionary relationships of flaviviruses. In Dengue and Dengue Hemorrhagic Fever (D.J. Gubler, ed.) pp. 147-173. CAB International, London, United Kingdom.
26. World Health Organization. 2009. Dengue: Guidelines for diagnosis, treatment, prevention and control.World Health Organization and the Special Programme for Research and Training in Tropical Diseases, Geneva.
27. World Health Organization, 2010. Impact of Dengue.http://www.who.int/csr/disease/dengue/impact/en/index.html.