Long term culture of MDCK strains alters chromosome content

BMC Research Notes

Volumn 6, Issue 1, 2013, Pages

  Cassio, Doris ^a,b  

^a INSERM   (France)

^b UNIVERSITÉ PARIS SACLAY   (France)

Author keywords

Cell line drift; Chromosome content; Madin Darby Canine Kidney (MDCK) cells; MDCK I; MDCK II; Metaphase

Indexed keywords

ANIMAL; ARTICLE; CHROMOSOME; CULTURE TECHNIQUE; DOG; LABORATORY DIAGNOSIS; MDCK CELL; METAPHASE; TIME; ULTRASTRUCTURE; CELL CULTURE TECHNIQUE; MDCK CELL LINE; SPECIMEN HANDLING; TIME FACTOR;

ANIMALS; CELL CULTURE TECHNIQUES; CHROMOSOMES; DOGS; MADIN DARBY CANINE KIDNEY CELLS; METAPHASE; SPECIMEN HANDLING; TIME FACTORS;

EID: 84876664958     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-6-162     Document Type: Article

References (12)

1. Characterization of an established line of canine kidney cells (MDCK). Gaush CR, Hard WL, Smith TF, Proc Soc Exp Biol Med 1966 122 3 931 935 5918973
2. Pubmed [ http://www.ncbi.nlm.nih.gov/pubmed?term=MDCKcells ]
3. The MDCK variety pack: choosing the right strain. Dukes JD, Whitley P, Chalmers AD, BMC Cell Biology 2011 12 43 10.1186/1471-2121-12-43 21982418
4. Pubmed [ http://www.ncbi.nlm.nih.gov/pubmed?term=MDCKandkaryotype ]
5. Phenotypically and karyotypically distinct Madin-Darby canine kidney cell clones respond differently to alkaline stress. Wünsch S, Gekle M, Kersting U, Schuricht B, Oberleithner H, J Cell Physiol 1995 164 1 164 171 10.1002/jcp.1041640121 7790388
6. Two strains of the Madin-Darby canine kidney (MDCK) cell line have distinct glycosphingolipid compositions. Hansson GC, Simons K, van Meer G, EMBO J 1986 5 3 483 489 3519211
7. The type 3 inositol 1,4,5-trisphosphate receptor is concentrated at the tight junction level in polarized MDCK cells. Colosetti P, Tunwell RE, Cruttwell C, Arsanto JP, Mauger JP, Cassio D, J Cell Sci 2003 116 Pt 13 2791 2803 12759372 (Pubitemid 36857230)
8. A General and Reliable Method for Obtaining High Yield Metaphasic Preparations from Adherent Cell Lines: Rapid Verification of Cell Chromosomal Content. Cassio D, Cell Biology, A Laboratory Handbook Waltham: Academic Press, Celis J, 3 2005 vol III, chapter 39
9. Karyotyping. Worton RG, Duff C, Methods Enzymol 1979 58 322 344 423774
10. Endoreplication: polyploidy with purpose. Lee HO, Davidson JM, Duronio RJ, Genes Dev 2009 23 21 2461 2477 10.1101/gad.1829209 19884253
11. Heterogeneity of the tumorigenic phenotype expressed by Madin-Darby canine kidney cells. Omeir RL, Teferedegne B, Foseh GS, Beren JJ, Snoy PJ, Brinster LR, Cook JL, Peden K, Lewis AM Jr, Comp Med 2011 61 3 243 250 21819694
12. The costs of using unauthenticated, over-passaged cell lines: how much more data do we need? Hughes P, Marshall D, Reid Y, Parkes H, Gelber C, Biotechniques 2007 43 5 575 584 10.2144/000112598 18072586 (Pubitemid 350174907)