Fabrication of microengineered templates and their applications into micropatterned cell culture

Journal of Biomedical Nanotechnology

Volumn 9, Issue 3, 2013, Pages 377-381

  Choi, Jin Ho ^a   Lee, Hyun ^a   Jin, Hee Kyung ^a   Bae, Jae Sung ^b   Kim, Gyu Man ^a  

^a Kyungpook National University   (South Korea)

^b Kyungpook National University School of Medicine   (South Korea)

Author keywords

Mammalian Cell; Microcontact Printing; Micropatterning; Microstencil; PDMS Microtemplate; Photolithography

Indexed keywords

MAMMALIAN CELLS; MICRO CONTACT PRINTING; MICRO PATTERNING; MICROSTENCIL; PDMS MICROTEMPLATE;

AMINO ACIDS; ANIMAL CELL CULTURE; FABRICATION; MICROCHANNELS; PHOTOLITHOGRAPHY; PRINTING;

ELECTRON DEVICE MANUFACTURE;

POLY DEXTRO LYSINE;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BIOENGINEERING; BRAIN CELL; CELL VIABILITY; CYTOTOXICITY; FIBROBLAST; IMMUNOCYTOCHEMISTRY; MICROTECHNOLOGY; MICROTEMPLATE; MOUSE; MOUSE EMBRYONIC FIBROBLAST; NICK END LABELING; NONHUMAN; SYNTHESIS;

ANIMALS; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CELLS, CULTURED; CEREBRAL CORTEX; DIMETHYLPOLYSILOXANES; EMBRYO, MAMMALIAN; EXTRACELLULAR MATRIX; FIBROBLASTS; IN SITU NICK-END LABELING; MICE; MICROSCOPY, FLUORESCENCE; MICROTECHNOLOGY; NEURONS;

EID: 84876591635     PISSN: 15507033     EISSN: 15507041     Source Type: Journal    
DOI: 10.1166/jbn.2013.1508     Document Type: Article

References (20)

1. S. R. Khetani and S. N. Bhatia, Microscale culture of human liver cells for drug development. Nature Biotechnology 26, 120 (2008).
2. J. Y. Lee, S. S. Shah, J. Yan, M. C. Howland, A. N. Parikh, T. Pan, and A. Revzin, Integrating sensing hydrogel microstructures into micropatterned hepatocellular cocultures. Langmuir 25, 3880 (2009).
3. J. H. Choi and G. M. Kim, Micro-patterning on non-planar surface using flexible microstencil, International Journal Of Precision Engineering And Manufacturing 12, 165 (2011).
4. K. Nakazawa, Y. Shinmura, Y. Yoshiura, and Y. Sakai, Effect of cell spot sizes on micropatterned cultures of rat hepatocytes. Biochemical Engineering Journal 53, 85 (2010).
5. Y. Sakai and K. Nakazawa, Technique for the control of spheroid diameter using microfabricated chips. Acta Biomaterialia 3, 1033 (2007).
6. J. Fukuda, A. Khademhosseini, Y. Yeo, X. Yang, J. Yeh, G. Eng, J. Blumling, C.-F. Wang, D. S. Kohane, and R. Langer, Micromolding of photocrosslinkable chitosan hydrogel for spheroid microarray and co-cultures. Biomaterials 27, 5259 (2006).
7. T. Tamura, Y. Sakai, and K. Nakazawa, Two-dimensional microarray of HepG2 spheroids using collagen/polyethylene glycol micropatterned chip. J. Mater. Sci: Mater Med. 19, 2071 (2008).
8. H.-F. Lu, K.-N. Chua, P.-C. Zhang, W.-S. Lim, S. Ramakrishna, K. W. Leong, and H.-Q. Mao, Three-dimensional co-culture of rat hepatocyte spheroids and NIH/3T3 fibroblasts enhances hepatocyte functional maintenance. Acta Biomaterialia 1, 399 (2005).
9. Y.-C. Toh, C. Zhang, J. Zhang, Y. M. Khong, S. Chang, V. D. Samper, D. van Noort, D. W. Hutmacher, and H. Yu, A novel 3D mammalian cell perfusion-culture system in microfluidic channels. Lab on a Chip. 7, 302 (2007).
10. B. Ma, G. Zhang, J. Qin, and B. Lin, Characterization of drug metabolites and cytotoxicity assay simultaneously using an integrated microfluidic device. Lab on a Chip. 9, 232 (2009).
11. S.-M. Ong, C. Zhang, Y.-C. Toh, S. H. Kim, H. L. Foo, C. H. Tan, D. van Noort, S. Park, and H. Yu, A gel-free 3D microfluidic cell culture system. Biomaterials 29, 3237 (2008).
12. L. J. Millet, M. E. Stewart, J. V. Sweedler, R. G. Nuzzo, and M. U. Gillette, Microfluidic devices for culturing primary mammalian neurons at low densities. Lab on a Chip. 7, 987 (2007).
13. J. R. Rettig and A. Folch, Large-scale single-cell trapping and imaging using microwell arrays. Anal. Chem. 77, 5628 (2005).
14. J. M. Karp, J. Yeh, G. Eng, J. Fukuda, J. Blumling, K.-Y. Suh, J. Cheng, A. Mahdavi, J. Borenstein, R. Langer, and A. Khademhosseini, Controlling size, shape and homogeneity of embryoid bodies using poly(ethylene glycol) microwells. Lab on a Chip. 7, 786 (2007).
15. H.-C. Moeller, M. K. Mian, S. Shrivastava, B. G. Chung, and A. Khademhosseini, A microwell array system for stem cell culture. Biomaterials 29, 752 (2008).
16. M. Ochsner, M. R. Dusseiller, H. M. Grandin, S. Luna-Morris, M. Textor, V. Vogel, and M. L. Smith, Micro-well arrays for 3D shape control and high resolution analysis of single cells. Lab on a Chip. 7, 1074 (2007).
17. J. C. Mohr, J. J. de Pablo, and S. P. Palecek, 3-D microwell culture of human embryonic stem cells. Biomaterials 27, 6032 (2006).
18. E. A. Roth, T. Xu, M. Das, C. Gregory, J. J. Hickman, and T. Boland, Inkjet printing for high-throughput cell patterning. Biomaterials 25, 3707 (2004).
19. S. Ilkhanizadeh, A. I. Teixeira, and O. Hermanson, Inkjet printing of macromolecules on hydrogels to steer neural stem cell differentiation. Biomaterials 28, 3936 (2007).
20. T. Yang, J. K. Knowles, Q. Lu, H. Zhang, O. Arancio, L. A. Moore, T. Chang, Q. Wang, K. Andreasson, J. Rajadas, G. G. Fuller, Y. Xie, S. M. Massa, and F. M. Longo, Small molecule, non-peptide p75NTR ligands inhibit Aβ-induced neurodegeneration and synaptic impairment. PLoS ONE 3, e3604 (2008).