A new biomaterial of nanofibers with the microalga spirulina as scaffolds to cultivate with stem cells for use in tissue engineering

Journal of Biomedical Nanotechnology

Volumn 9, Issue 4, 2013, Pages 710-718

  Steffens, D ^a   Lersch, M ^a   Rosa, A ^a   Scher, C ^a   Crestani, T ^a   Morais, M G ^b   Costa, J A V ^b   Pranke, P ^a,c  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^b FEDERAL UNIVERSITY OF RIO GRANDE   (Brazil)

^c Stem Cell Research Institute   (Brazil)

Author keywords

Nanotechnology; Scaffolds; Spirulina; Stem Cells; Tissue Engineering

Indexed keywords

BIOACTIVE COMPONENTS; ELECTROSPINNING TECHNIQUES; EXTRACELLULAR MATRICES; PHYSICO-CHEMICAL ANALYSIS; POLY-D ,L-LACTIC ACID; REGENERATIVE MEDICINE; SPIRULINA; TOPOGRAPHICAL PROPERTIES;

BIOMATERIALS; CONTACT ANGLE; NANOFIBERS; NANOTECHNOLOGY; SCAFFOLDS; STEM CELLS; TISSUE; TISSUE ENGINEERING;

SCAFFOLDS (BIOLOGY);

BIOMATERIAL; MOLECULAR SCAFFOLD; NANOFIBER; POLYLACTIC ACID;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIODEGRADABILITY; BIOMASS; CELL ADHESION; CELL PROLIFERATION; CELL STRUCTURE; CELL VIABILITY; CONTACT ANGLE; DRUG CYTOTOXICITY; ELECTROSPINNING; EXTRACELLULAR MATRIX; HYDROPHOBICITY; IN VITRO STUDY; MESENCHYMAL STEM CELL; MICROALGA; MOUSE; NONHUMAN; PARTICLE SIZE; PHYSICAL CHEMISTRY; SPIRULINA; TISSUE ENGINEERING; VOLATILIZATION;

ANIMALS; BIOCOMPATIBLE MATERIALS; BIOMASS; CELL ADHESION; CELL DEATH; CELL SURVIVAL; CELLS, CULTURED; L-LACTATE DEHYDROGENASE; LACTIC ACID; MESENCHYMAL STROMAL CELLS; MICE; MICROALGAE; MOLECULAR WEIGHT; NANOFIBERS; POLYMERS; SOLVENTS; SPECTROMETRY, FLUORESCENCE; SPIRULINA; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

EID: 84876575921     PISSN: 15507033     EISSN: 15507041     Source Type: Journal    
DOI: 10.1166/jbn.2013.1571     Document Type: Article

References (50)

1. A. D. Metcalfe and M. W. Ferguson, Tissue engineering of replacement skin: The crossroads of biomaterials, wound healing, embryonic development, stem cells and regeneration. J. R. Soc. Interface 4, 413 (2007).
2. F. R. Huss, E. Nyman, C. J. Gustafson, K. Gisselfalt, E. Liljensten, and G. Kratz, Characterization of a new degradable polymer scaffold for regeneration of the dermis: In vitro and in vivo human studies. Organogenesis 4, 195 (2008).
3. J. Stitzel, J. Liu, S. J. Lee, M. Komura, J. Berry, S. Soker, G. Lim, M. Van Dyke, R. Czerw, J. J. Yoo, and A. Atala, Controlled fabrication of a biological vascular substitute. Biomaterials. 27, 1088 (2006).
4. O. E. Teebken, C. Puschmann, B. Rohde, K. Burgwitz, M. Winkler, A. M. Pichlmaier, J. Weidemann, and A. Haverich, Human iliac vein replacement with a tissue-engineered graft. Vasa. 38, 60 (2009).
5. V. Thomas, M. V. Jose, S. Chowdhury, J. F. Sullivan, D. R. Dean, and Y. K. Vohra, Mechano-morphological studies of aligned nanofibrous scaffolds of polycaprolactone fabricated by electrospinning. J. Biomater. Sci. Polym. Ed. 17, 969 (2006).
6. G. Sui, X. Yang, F. Mei, X. Hu, G. Chen, X. Deng, and S. Ryu, Poly-L-lactic acid/hydroxyapatite hybrid membrane for bone tissue regeneration. J. Biomed. Mater. Res. A 82, 445 (2007).
7. E. Schnell, K. Klinkhammer, S. Balzer, G. Brook, D. Klee, P. Dalton, and J. Mey, Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-epsilon-caprolactone and a collagen/poly-epsilon-caprolactone blend. Biomaterials 28, 3012 (2007).
8. F. Yang, R. Murugan, S. Wang, and S. Ramakrishna, Electrospinning of nano/micro scale poly(L-lactic acid) aligned fibers and their potential in neural tissue engineering. Biomaterials 26, 2603 (2005).
9. L. Zheng, J. Sun, X. Chen, G. Wang, B. Jiang, H. Fan, and X. Zhang, In vivo cartilage engineering with collagen hydrogel and allogenous chondrocytes after diffusion chamber implantation in immunocompetent host. Tissue Eng. Part A 15, 2145 (2009).
10. G. Zhao, S. Yin, G. Liu, L. Cen, J. Sun, H. Zhou, W. Liu, L. Cui, and Y. Cao, In vitro engineering of fibrocartilage using CDMP1 induced dermal fibroblasts and polyglycolide. Biomaterials 30, 3241 (2009).
11. C. H. Lee, H. J. Shin, I. H. Cho, Y. M. Kang, I. A. Kim, K. D. Park, and J. W. Shin, Nanofiber alignment and direction of mechanical strain affect the ECM production of human ACL fibroblast. Biomaterials 26, 1261 (2005).
12. S. Agarwal, J. H. Wendorff, and A. Greiner, Progress in the field of electrospinning for tissue engineering applications. Adv. Mater. 21, 3343 (2009).
13. M. Miura, S. Gronthos, M. Zhao, B. Lu, L. W. Fisher, P. G. Robey, and S. Shi, SHED: Stem cells from human exfoliated deciduous teeth. Proc. Natl. Acad. Sci. USA 100, 5807 (2003).
14. X. L. Ju, Z. W. Huang, Q. Shi, H. S. Hou, and C. H. Duan, Biological characteristics and induced differentiation ability of in vitro expanded umbilical cord blood mesenchymal stem cells. Zhonghua Er Ke Za Zhi. 43, 499 (2005).
15. E. Çatiker, M. Gümüpderelioǧlu, and A. Güner, Degradation of PLA, PLGA homo- and copolymers in the presence of serum albumin: A spectroscopic investigation. Polym. Int. 49, 728 (2000).
16. L. Jin, T. Wang, M. L. Zhu, M. K. Leach, Y. I. Naim, J. M. Corey, Z. Q. Feng, and Q. Jiang, Electrospun fibers and tissue engineering. J. Biomed. Nanotechnol. 8, 1 (2012).
17. M. G. de Morais, C. Stillings, R. Dersch, M. Rudisile, P. Pranke, J. A. Costa, and J. Wendorff, Preparation of nanofibers containing the microalga Spirulina (Arthrospira). Bioresour. Technol. 101, 2872 (2010).
18. Z. Khan, P. Bhadouria, and P. S. Bisen, Nutritional and therapeutic potential of Spirulina. Curr. Pharm. Biotechnol. 6, 373 (2005).
19. P. D. Karkos, S. C. Leong, C. D. Karkos, N. Sivaji, and D. A. Assimakopoulos, Spirulina in clinical practice: Evidence-based human applications. Evid Based Complement Alternat Med. (2008).
20. A. Kulshreshtha, A. J. Zacharia, U. Jarouliya, P. Bhadauriya, G. B. Prasad, and P. S. Bisen, Spirulina in health care management. Curr. Pharm. Biotechnol. 9, 400 (2008).
21. L. da Silva Meirelles, P. C. Chagastelles, and N. B. Nardi, Mesenchymal stem cells reside in virtually all post-natal organs and tissues. J. Cell Sci. 119, 2204 (2006).
22. B. Saad, S. Dakwar, O. Said, G. Abu-Hijleh, F. Al Battah, A. Kmeel, and H. Aziazeh, Evaluation of medicinal plant hepatotoxicity in co-cultures of hepatocytes and monocytes. Evid Based Complement Alternat Med. 3, 93 (2006).
23. B. Saad, G. Abu-Hijleh, and U. W. Suter, Cell culture techniques for assessing tissue compatibility of biomaterials, Introduction Polymeric Biomaterials, R, A. Ed., The Citus Books, London (2003), Vol. I, pp. 263-299.
24. D. L. Nelson and M. M. Cox, Fotossíntese: Captura Da Energia Luminosa, Lehninger Principios de Bioquímica, 3th edn., Sarvier: São Paulo (2002), p. 975.
25. G. Vunjak-Novakovic and M. Radisic, Cell seeding of polymer scaffolds. Methods Mol. Biol. 238, 131 (2004).
26. H. Shearer, M. J. Ellis, S. P. Perera, and J. B. Chaudhuri, Effects of common sterilization methods on the structure and properties of poly(D,L lactic-co-glycolic acid) scaffolds. Tissue Eng. 12, 2717 (2006).
27. L. Zhao, C. He, Y. Gao, L. Cen, L. Cui, and Y. Cao, Preparation and cytocompatibility of PLGA scaffolds with controllable fiber morphology and diameter using electrospinning method. J. Biomed. Mater. Res. B Appl. Biomater. 87, 26 (2008).
28. Y. D. Teng, E. B. Lavik, X. Qu, K. I. Park, J. Ourednik, D. Zurakowski, R. Langer, and E. Y. Snyder, Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells. Proceedings of the National Academy of Sciences of the United States of America 99, 3024 (2002).
29. C. P. Barnes, S. A. Sell, E. D. Boland, D. G. Simpson, and G. L. Bowlin, Nanofiber technology: Designing the next generation of tissue engineering scaffolds. Adv. Drug Deliv. Rev. 59, 1413 (2007).
30. C. Wang, H. Chien, K. Yan, C. Hung, K. Hung, S. Tsai, and H. Jhang, Correlation between processing parameters and microstructure of electrospun poly(D,L-lactic acid) nanofibers. Polymer 50, 6100 (2009).
31. W. J. Li, J. A. Cooper, Jr, R. L. Mauck, and R. S. Tuan, Fabrication and characterization of six electrospun poly(alpha-hydroxy ester)- based fibrous scaffolds for tissue engineering applications. Acta Biomater. 2, 377 (2006).
32. J. A. Roether, A. R. Boccaccini, L. L. Hench, V. Maquet, S. Gautier, and R. Jerjme, Development and in vitro characterisation of novel bioresorbable and bioactive composite materials based on polylactide foams and Bioglass for tissue engineering applications. Biomaterials 23, 3871 (2002).
33. J. J. Blaker, J. E. Gough, V. Maquet, I. Notingher, and A. R. Boccaccini, In vitro evaluation of novel bioactive composites based on Bioglass-filled polylactide foams for bone tissue engineering scaffolds. J. Biomed. Mater. Res. A 67, 1401 (2003).
34. S. Verrier, J. J. Blaker, V. Maquet, L. L. Hench, and A. R. Boccaccini, PDLLA/Bioglass composites for soft-tissue and hardtissue engineering: An in vitro cell biology assessment. Biomaterials 25, 3013 (2004).
35. V. Maquet, A. R. Boccaccini, L. Pravata, I. Notingher, and R. Jerome, Porous poly(alpha-hydroxyacid)/Bioglass composite scaffolds for bone tissue engineering. I: Preparation and in vitro characterisation. Biomaterials 25, 4185 (2004).
36. G. Zanatta, M. Rudisile, M. Camassola, J. Wendorff, N. Nardi, C. Gottfried, P. Pranke, and C. A. Netto, Mesenchymal stem cell adherence on poly(D, L-lactide-co-glycolide) nanofibers scaffold is integrin-beta 1 receptor dependent. J. Biomed. Nanotechnol. 8, 211 (2012).
37. K. D. Andrews, J. A. Hunt, and R. A. Black, Effects of sterilisation method on surface topography and in-vitro cell behaviour of electrostatically spun scaffolds. Biomaterials 28, 1014 (2007).
38. F. Gentile, L. Tirinato, E. Battista, F. Causa, C. Liberale, E. M. di Fabrizio, and P. Decuzzi, Cells preferentially grow on rough substrates. Biomaterials 31, 7205 (2010).
39. S. Sahoo, H. Ouyang, J. C. Goh, T. E. Tay, and S. L. Toh, Characterization of a novel polymeric scaffold for potential application in tendon/ligament tissue engineering. Tissue Eng. 12, 91 (2006).
40. G. Jin, M. P. Prabhakaran, and S. Ramakrishna, Stem cell differentiation to epidermal lineages on electrospun nanofibrous substrates for skin tissue engineering. Acta Biomater. 7, 3113 (2011).
41. J. D. Wei, H. Tseng, E. T. Chen, C. H. Hung, Y. C. Liang, M. T. Sheu, and C. H. Chen, Characterizations of chondrocyte attachment and proliferation scaffolds of PLLA and PBSA for Use in cartilage tissue engineering. J. Biomater. Appl. 26, 963 (2011).
42. S. H. Ku and C. B. Park, Human endothelial cell growth on musselinspired nanofiber scaffold for vascular tissue engineering. Biomaterials 31, 9431 (2010).
43. A. R. Pohlmann, C. O. Petter, N. M. Balzaretti, and S. S. Guterres, Tópicos em Nanociência e Nanotecnologia, Editora da Universidade Federal do Rio Grande do Sul: Porto Alegre (2008), Vol. I, p. 243.
44. R. P. Pohanish, Sittig's Handbook of Toxic and Hazardous Chemicals and Carcinogens, 6th edn., Elsevier Inc. (2012), p. 3,000.
45. E. M. I. S. I. G.-. EMISIG, Protective Action Criteria (PAC) with AEGLs, ERPGs, & TEELs: Rev. 26 for Chemicals of Concern (09/2010). http://www.atlintl.com/DOE/teels/teel/complete.asp (11-30).
46. M. Navarro, C. Aparicio, M. Charles-Harris, M. P. Ginebra, E. Engel, and J. A. Planell, Development of a biodegradable composite scaffold for bone tissue engineering: Physicochemical, topographical, mechanical, degradation, and biological properties. Adv. Polym. Sci. 200, 209 (2006).
47. S. Sahoo, L. T. Ang, J. C. Goh, and S. L. Toh, Growth factor delivery through electrospun nanofibers in scaffolds for tissue engineering applications. J. Biomed. Mater. Res. A 93, 1539 (2010).
48. X. Li, Y. Su, S. Liu, L. Tan, X. Mo, and S. Ramakrishna, Encapsulation of proteins in poly(L-lactide-co-caprolactone) fibers by emulsion electrospinning. Colloids Surf. B Biointerfaces 75, 418 (2010).
49. M. A. Qureshi and R. A. Ali, Spirulina platensis exposure enhances macrophage phagocytic function in cats. Immunopharmacol Immunotoxicol. 18, 457 (1996).
50. M. A. Qureshi, J. D. Garlich, and M. T. Kidd, Dietary spirulina platensis enhances humoral and cell-mediated immune functions in chickens. Immunopharmacol Immunotoxicol. 18, 465 (1996).