Monitoring GPCR-Protein Complexes Using Bioluminescence Resonance Energy Transfer

G Protein-Coupled Receptors: Essential Methods

Volumn , Issue , 2010, Pages 111-132

  Jaeger, Werner C ^a   Pfleger, Kevin D G ^a   Eidne, Karin A ^a  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

BRET expression systems and fusion protein transfection; BRET principles; Choice of BRET method and procedure; Conventional biochemical methods at detecting protein protein interactions; Formation of multiple protein complexes activation life cycle of G protein coupled receptors (GPCRs); Fourier transform infrared spectroscopy (FTIR) and biological macromolecule structure; Low or no BRET signal; Low or no protein expression; Measuring GPCR protein interactions using BRET; Monitoring GPCR protein complexes using bioluminescence resonance energy transfer

Indexed keywords

EID: 84876490105     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9780470749210.ch6     Document Type: Chapter

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