Allele dropout caused by a non-primer-site SNV affecting PCR amplification - A call for next-generation primer design algorithm

Clinica Chimica Acta

Volumn 421, Issue , 2013, Pages 208-212

  Lam, Ching Wan ^a   Mak, Chloe Miu ^b  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

^b PRINCESS MARGARET HOSPITAL   (Hong Kong)

Author keywords

Allele dropout; Apparent homozygosity; FAH gene; Hairpin formation; Next generation primer design algorithm; Non primer site SNV

Indexed keywords

FUMARYLACETOACETASE;

ALLELE; ALLELE DROPOUT; ARTICLE; BINDING SITE; CASE REPORT; COMPUTER PROGRAM; DNA HAIRPIN; FAH GENE; GENE; GENE DELETION; GENE FREQUENCY; GENETIC ALGORITHM; GENETIC ANALYSIS; GENETIC VARIABILITY; HOMOZYGOSITY; HUMAN; NEXT GENERATION PRIMER DESIGN ALGORITHM; NON PRIMER SINGLE NUCLEOTIDE VARIANT; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STRUCTURE ANALYSIS; TYROSINEMIA;

ALGORITHMS; ALLELES; ARTIFACTS; DNA PRIMERS; GENOTYPE; GENOTYPING TECHNIQUES; HOMOZYGOTE; HUMANS; HYDROLASES; INVERTED REPEAT SEQUENCES; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PEDIGREE; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; SOFTWARE; TYROSINEMIAS;

EID: 84876402419     PISSN: 00098981     EISSN: 18733492     Source Type: Journal    
DOI: 10.1016/j.cca.2013.03.014     Document Type: Article

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