Species-specific PCR improves detection of bacterial pathogens in parapneumonic empyema compared with 16SPCR and culture

Pediatric Infectious Disease Journal

Volumn 32, Issue 3, 2013, Pages 302-303

  Blaschke, Anne J ^a   Byington, Carrie L ^a   Ampofo, Krow ^a   Pavia, Andrew T ^a   Heyrend, Caroline ^a   Rankin, Shelley C ^b   McGowan, Karin L ^b   Harris, Mary C ^b   Shah, Samir S ^c  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c CINCINNATI CHILDREN'S HOSPITAL MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM CULTURE; BACTERIUM DETECTION; DIAGNOSTIC ACCURACY; EMPYEMA; HUMAN; LETTER; MCNEMAR TEST; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; NONHUMAN; PARAPNEUMONIC EMPYEMA; PATIENT SELECTION; PLEURA FLUID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STREPTOCOCCUS PNEUMONIAE; UNITED STATES;

BACTERIA; BACTERIAL INFECTIONS; CHILD; CHILD, PRESCHOOL; EMPYEMA, PLEURAL; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; PNEUMONIA, BACTERIAL; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 84876233401     PISSN: 08913668     EISSN: 15320987     Source Type: Journal    
DOI: 10.1097/INF.0b013e3182784ec1     Document Type: Letter

References (5)

1. Grijalva CG, Nuorti JP, Zhu Y, et al. Increasing incidence of empyema complicating childhood community-acquired pneumonia in the United States. Clin Infect Dis. 2010;50:805-813.
2. Le Monnier A, Carbonnelle E, Zahar JR, et al. Microbiological diagnosis of empyema in children: comparative evaluations by culture, polymerase chain reaction, and pneumococcal antigen detection in pleural fluids. Clin Infect Dis. 2006;42:1135-1140.
3. Saglani S, Harris KA, Wallis C, et al. Empyema: the use of broad range 16S rDNA PCR for pathogen detection. Arch Dis Child. 2005;90:70-73.
4. Blaschke AJ, Heyrend C, Byington CL, et al. Molecular analysis improves pathogen identification and epidemiologic study of pediatric parapneumonic empyema. Pediatr Infect Dis J. 2011;30:289-294.
5. Gollomp K, Rankin SC, White C, et al. Broad-range bacterial polymerase chain reaction in the microbiologic diagnosis of complicated pneumonia. J Hosp Med. 2012;7:8-13.