"salt tolerant" anion exchange chromatography for direct capture of an acidic protein from CHO cell culture

Protein Expression and Purification

Volumn 89, Issue 2, 2013, Pages 117-123

  Champagne, Jérôme ^a   Balluet, Guillaume ^a   Gantier, René ^b   Toueille, Magali ^a  

^a Pall Life Sciences   (France)

^b Pall Life Sciences   (United States)

Author keywords

Amylase; Anion exchange chromatography; CHO proteins; Flexibility; Productivity; Salt tolerance

Indexed keywords

AMYLASE; ANION; INORGANIC SALT;

ADSORPTION; ANIMAL; ARTICLE; CHEMISTRY; CHO CELL; CRICETULUS; ENZYME STABILITY; HAMSTER; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY;

ADSORPTION; ALPHA-AMYLASES; ANIMALS; ANIONS; CHO CELLS; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; ENZYME STABILITY; SALTS;

CRICETULUS GRISEUS;

EID: 84876109465     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.03.005     Document Type: Article

References (19)

1. A.A. Shukla, B. Hubbard, T. Tressel, S. Guhan, and D. Low Downstream processing of monoclonal antibodies - application of platform approaches J. Chromatogr. B 848 2007 28 39 (Pubitemid 46367766)
2. A. Arunakumari, and J. Wang Purification of human monoclonal antibodies: non-protein A strategies U. Gottschalk, Process Scale Purification of Antibodies 2009 John Wiley & Sons Hoboken, NJ 102 123
3. T. Burnouf Chromatography in plasma fractionation: benefits and future trends J. Chromatogr. B. 664 1995 3 15
4. B.J. Min, S.W. Kang, Y.S. Song, J.H. Lee, S.W. Kim, C. Park, and C.W. Kim Validation for capture anion exchange chromatography process in r-hGH manufacturing Process Biochem. 43 2008 808 815
5. A.M. Hardin, C. Harinarayan, G. Malmquist, A. Axén, and R. van Reis Ion exchange chromatography of monoclonal antibodies: effect of resin ligand density on dynamic binding capacity J. Chromatogr. A 1216 2009 4366 4371
6. P.R. Levison Proteins/ion exchange Ian.D. Wilson, Encyclopedia of Separation Science 2000 Academic Press Oxford 4047 4052
7. A. Staby, I.H. Jensen, and I. Mollerup Comparison of chromatographic ion-exchange resins: I. Strong anion-exchange resins J. Chromatogr. A. 897 2000 99 111
8. P. Gagnon How to choose an industrial cation exchanger for IgG purification Bioprocess Int. 8 2010 22 34
9. J. Pezzini, C. Cabanne, and X. Santarelli Comparative study of strong anion exchangers: structure-related chromatographic performances J. Chromatogr. B. 877 2009 2443 2450
10. H. Lutz, and B. Raghunath Ultrafiltration process design and implementation A.A. Shukla, M.R. Etzel, S. Gadam, Process Scale Bioseparation for the Biopharmaceutical Industry 2007 CRC Press Boca Raton 297 332
11. U. Gottschalk Overview of downstream processing in the biomanufacturing industry M. Moo-Young, Comprehensive Biotechnology second ed. 2011 Academic Press Burlington 669 682
12. S.S. Farid Process economics of industrial monoclonal antibody manufacture J. Chromatogr. B. 848 2007 8 18 (Pubitemid 46367764)
13. C. Han, P. Nelson, and A. Tsai Process development's impact on cost of goods manufactured (COGM) BioProcess Int. 8 2010 48 55
14. B.-L. Johansson, M. Belew, S. Eriksson, G. Glad, O. Lind, J.-L. Maloisel, and N. Norrman Preparation and characterization of prototypes for multi-modal separation media aimed for capture of negatively charged biomolecules at high salt conditions J. Chromatogr. A 1016 2003 21 33 (Pubitemid 37123190)
15. J.J. Pierce, S.C. Robinson, J.M. Ward, E. Keshavarz-Moore, and P. Dunnill Comparison of the process issues in expressing the same recombinant enzyme periplasmically in Escherichia coli and extracellularly in Streptomyces lividans J. Biotechnol. 92 2002 205 215 (Pubitemid 33022625)
16. S. Sivaramakrishnan, D. Gangadharan, K.M. Nampoothiri, C.R. Soccol, and A. Pandey α-Amylases from microbial Sources - an overview on recent developments Food Technol. Biotechnol. 44 2006 173 184 (Pubitemid 44033841)
17. M.J.E.C. van der Maarel, B. van der Veen, J.C.M. Uitdehaag, H. Leemhuis, and L. Dijkhuizen Properties and applications of starch-converting enzymes of the α-amylase family J. Biotechnol. 94 2002 137 155 (Pubitemid 34127657)
18. A. Burhan, U. Nisa, C. Gökhan, C. Ömer, A. Ashabil, and G. Osman Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6 Process Biochem. 38 2003 1397 1403 (Pubitemid 36725911)
19. S.R. Holmes-Farley, W.H. Mandeville, J. Ward, and K.L. Miller Design and characterization of sevelamer hydrochloride: a novel phosphate-binding pharmaceutical J. Macromol. Sci. Pure Appl. Chem. 36 1999 1085 1091 (Pubitemid 129613826)