Expression level of bacillus subtilis germinant receptors determines the average rate but not the heterogeneity of spore germination

Journal of Bacteriology

Volumn 195, Issue 8, 2013, Pages 1735-1740

  Zhang, Jing qiao ^a   Griffiths, Keren K ^a   Cowan, Ann ^a   Setlow, Peter ^a   Yu, Ji ^a  

^a UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GERMINANT RECEPTOR; RECEPTOR; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; CELL MEMBRANE; CONTROLLED STUDY; GENE EXPRESSION; GENETIC HETEROGENEITY; HYPOTHESIS; NONHUMAN; PRIORITY JOURNAL; SPORE GERMINATION; STATISTICS; THEORETICAL MODEL;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CELL MEMBRANE; GENE EXPRESSION REGULATION, BACTERIAL; MEMBRANE PROTEINS; MODELS, BIOLOGICAL; PICOLINIC ACIDS; RECEPTORS, CELL SURFACE; SPORES, BACTERIAL; STOCHASTIC PROCESSES; TIME FACTORS;

BACILLUS SUBTILIS;

EID: 84876091925     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.02212-12     Document Type: Article

References (26)

1. Setlow P. 2003. Spore germination. Curr. Opin. Microbiol. 6:550-556.
2. Setlow P. 1988. Small acid-soluble spore proteins of Bacillus species: structure, synthesis, genetics, function, and degradation. Annu. Rev. Microbiol. 42:319-338.
3. Paidhungat M, Setlow P. 2000. Role of Ger proteins in nutrient and nonnutrient triggering of spore germination in Bacillus subtilis. J. Bacte-riol. 182:2513-2519.
4. Hudson KD, Corfe BM, Kemp EH, Feavers IM, Coote PJ, Moir A. 2001. Localization of GerAA and GerAC germination proteins in the Bacillus subtilis spore. J. Bacteriol. 183:4317-4322.
5. Paidhungat M, Setlow P. 2001. Localization of a germinant receptor protein (GerBA) to the inner membrane of Bacillus subtilis spores. J. Bacteriol. 183:3982-3990.
6. Paredes-Sabja D, Setlow P, Sarker MR. 2009. Role of GerKB in germination and outgrowth of Clostridium perfringens spores. Appl. Environ. Microbiol. 75:3813-3817.
7. Griffiths KK, Zhang J, Cowan AE, Yu J, Setlow P. 2011. Germination proteins in the inner membrane of dormant Bacillus subtilis spores colo-calize in a discrete cluster. Mol. Microbiol. 81 (4):1061-1077.
8. Wang G, Yi X, Li YQ, Setlow P. 2011. Germination of individual Bacillus subtilis spores with alterations in the GerD and SpoVA proteins, which are importantin spore germination. J. Bacteriol. 193:2301-2311.
9. Chen D, Huang SS, Li YQ. 2006. Real-time detection of kinetic germination and heterogeneity of single Bacillus spores by laser tweezers Raman spectroscopy. Anal. Chem. 78:6936-6941.
10. Huang SS, Chen D, Pelczar PL, Vepachedu VR, Setlow P, Li YQ. 2007. Levels of Ca2+-dipicolinic acid in individual Bacillus spores determined using microfluidic Raman tweezers. J. Bacteriol. 189:4681-4687.
11. Kong L, Zhang P, Setlwo P, Li YQ. 2010. Characterization of bacterial spore germination using integrated phase contrast microscopy, Raman spectroscopy, and optical tweezers. Anal. Chem. 82:3840-3847.
12. Peng L, Chen D, Setlow Li YQ. 2009. Elastic and inelastic light scattering from single bacterial spores in an optical trap allows the monitoring of spore germination dynamics. Anal. Chem. 81:4035-4042.
13. Zhang P, Kong L, Wang G, Setlow P, Li YQ. 2010. Combination of Raman tweezers and quantitative differential interference contrast microscopy for measurement of dynamics and heterogeneity during the germination of individual bacterial spores. J. Biomed. Opt. 15:056010. doi:10.1117/1.3494567.
14. Gould GW. 1969. Spore germination, p 397-444. In Gould GW, Hurst A (ed), The bacterial spore. Academic Press, New York, NY.
15. Woese CR, Vary JC, Halvorson HO. 1968. A kinetic model for bacterial spore germination. Proc. Natl. Acad. Sci. U. S. A 59:869-875.
16. Moir A. 2006. How do spores germinate? J. Appl. Microbiol. 101:526-530.
17. Nicholson WL, Setlow P. 1990. Sporulation, germination and outgrowth, p 391-450. In Harwood CR, Cutting SM (ed), Molecular biological methods for Bacillus. John Wiley and Sons, Chichester, England.
18. Zhang J, Garner W, Setlow P, Yu J. 2011. Quantitative analysis of spatial-temporal correlations during germination of spores of Bacillus species. J. Bacteriol. 193:3765-3772.
19. Friedman N, Cai L, Xie XS. 2006. Linking stochastic dynamics to population distribution: an analytical framework of gene expression. Phys. Rev. Lett. 97:168302.
20. Taniguchi Y, Choi PJ, Li GW, Chen H, Babu M, Hearn J, Emili A, Xie XS. 2010. Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329:533-538.
21. Bagyan I, Setlow P. 2002. Localization of the cortex lytic enzyme CwlJ in spores of Bacillus subtilis. J. Bacteriol. 184:1219-1224.
22. Popham DL, Meador-Parton J, Costello CE, Setlow P. 1999. Spore peptidoglycan structure in a cwlD dacB double mutant of Bacillus subtilis. J. Bacteriol. 181:6205-6209.
23. Cabrera-Martinez RM, Tovar-Rojo F, Vepachedu VR, Setlow P. 2003. Effects of overexpression of nutrient receptors on germination of spores of Bacillus subtilis. J. Bacteriol. 185:2457-2464.
24. Pelczar PL, Setlow P. 2008. Localization of the germination protein GerD to the inner membrane in Bacillus subtilis spores. J. Bacteriol. 190:5635-5641.
25. Vepachedu VR, Setlow P. 2007. Analysis of interactions between nutrient germinant receptors and SpoVA proteins of Bacillus subtilis spores. FEMS Microbiol. Lett. 274:42-47.
26. Vepachedu VR, Setlow P. 2005. Localization of SpoVAD to the inner membrane of spores of Bacillus subtilis. J. Bacteriol. 187:5677-5682.