Microsomal prostaglandin E synthase type 2 (mPGES2) is a glutathione-dependent heme protein, and dithiothreitol dissociates the bound heme to produce active prostaglandin E2 synthase in vitro

Journal of Biological Chemistry

Volumn 288, Issue 14, 2013, Pages 10166-10175

  Takusagawa, Fusao ^a  

^a UNIVERSITY OF KANSAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2-MERCAPTOETHANOL; ABSORPTION AND FLUORESCENCE SPECTROSCOPY; CULTURE MEDIUM; DISSOCIATION CONSTANT; DITHIOTHREITOL; HEME OXIDATION; MICROSOMAL PROSTAGLANDIN; PROSTAGLANDINE;

BIOSYNTHESIS; DISSOCIATION; FLUORESCENCE SPECTROSCOPY; LIPIDS; PEPTIDES; PHOTODEGRADATION;

PORPHYRINS;

CELL EXTRACT; DITHIOTHREITOL; GLUTATHIONE; IMIDAZOLE; MERCAPTOETHANOL; PROSTAGLANDIN E SYNTHASE; PROSTAGLANDIN E SYNTHASE 2; PROSTAGLANDIN E2; PROSTAGLANDIN E2 SYNTHASE; UNCLASSIFIED DRUG;

ABSORPTION SPECTROSCOPY; ARTICLE; CRYSTAL STRUCTURE; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; IN VITRO STUDY; IN VIVO STUDY; LIVER; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; PRIORITY JOURNAL; PROTEIN BINDING;

ANIMALS; CATALYSIS; DITHIOTHREITOL; ENZYME ACTIVATION; ESCHERICHIA COLI; GLUTATHIONE; HEME; HYDROGEN PEROXIDE; INTRAMOLECULAR OXIDOREDUCTASES; MACACA FASCICULARIS; MERCAPTOETHANOL; MICROSOMES; MODELS, MOLECULAR; PROTEIN BINDING; SWINE;

MALIA; SUIDAE;

EID: 84875973395     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M112.418475     Document Type: Article

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