Ooptimization of PCR-RFLP directly from the skin and nails in cases of dermatophy-tosis, targeting the ITS and the 18s ribosomal DNA regions

Journal of Clinical and Diagnostic Research

Volumn 7, Issue 4, 2013, Pages 646-651

  Elavarashi, Elangovan ^a   Kindo, Anupma Jyoti ^a   Kalyani, Jagannathan ^b  

^a SRI RAMACHANDRA UNIVERSITY   (India)

^b ENT Hospital and Research Institute   (India)

Author keywords

Dermatophytosis; DNA sequencing; Genotyping; PCR; RFLP

Indexed keywords

DDE I ENZYME; DNA 18S; FUNGAL ENZYME; INTERNAL TRANSCRIBED SPACER; MVA I ENZYME; UNCLASSIFIED DRUG;

ADULT; AGED; ARTICLE; CHILD; DERMATOPHYTE; DERMATOPHYTOSIS; DNA SEQUENCE; EPIDERMOPHYTON FLOCCOSUM; FEMALE; FUNGAL STRAIN; FUNGUS IDENTIFICATION; GENOTYPE; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; MICROSCOPY; NAIL; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRESCHOOL CHILD; PROCESS OPTIMIZATION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SENSITIVITY AND SPECIFICITY; SKIN CULTURE; TRICHOPHYTON MENTAGROPHYTES; TRICHOPHYTON RUBRUM;

ARTHRODERMA BENHAMIAE; ARTHRODERMATACEAE; EPIDERMOPHYTON FLOCCOSUM; LATES; TRICHOPHYTON INTERDIGITALE;

EID: 84875878471     PISSN: 2249782X     EISSN: 0973709X     Source Type: Journal    
DOI: 10.7860/JCDR/2013/5363.2873     Document Type: Article

References (25)

1. Weitzman I, Summerbell RC. The dermatophytes. Clin Microbiol Rev. 1995; 8: 240-59.
2. Simpanya MF. Dermatophytes: Their Taxonomy ecology and Patho- genicity. In: Biology of Dermatophytes and other keratinophilic fungi. Kushwaha RKS and Guarro J, editors. Revista Iberoamericana de Micología, Bilbao. 2000; 1-12.
3. Havlickova B, Czaika VA, Friedrich M. Epidemiological trends in skin mycoses worldwide. Mycoses. 2008; 51: 2-15.
4. Ameen M. Epidemiology of superficial fungal infections. Clin Derma- tol. 2010; 28:197-201.
5. Sahai S, Mishra D. Change in spectrum of dermatophytes isolated from superficial mycoses cases: First report from Central India. In- dian J Dermatol Venereol Leprol. 2011; 77: 335-36.
6. Grumbt M, Monod M, Staib P. Genetic advances in dermatophytes. FEMS Microbiol Lett. 2011; 320: 79-86.
7. Martinez DA, Oliver BG, Gräser Y, Goldberg JM, Li W, et al. Compara- tive genome analysis of Trichophyton rubrum and related dermato- phytes reveals candidate genes involved in infection. mBio. 2012; 3: e00259-12.
8. Liu D, Coloe S, Pedersen J, Baird R. Use of arbitrarily primed poly- merase chain reaction to differentiate Trichophyton dermatophytes. FEMS Microbiol Lett. 1996; 136: 147-50.
9. Howell SA, Barnard RJ, Humphreys F. Application of molecular typ- ing methods to dermatophytes species that cause skin and nail in- fections. J Med Microbiol. 1999; 48: 33-40.
10. Baeza LC, Giannini MJ. Strain differentiation of Trichophyton rubrum Elangovan Elavarashi et al., PCR-RFLP from Clinical Specimens by random amplification of polymorphic DNA (RAPD). Rev Inst Med Trop S Paulo. 2004; 46: 339-41.
11. Pounder JI, Williams S, Hansen D, Healy M, Reece K, Woods GL. Repetitive-sequence-PCR-based DNA fingerprinting using the Diver- silab system for identification of commonly encountered dermato- phytes. J Clin Microbiol. 2005; 43: 2141-47.
12. Mochizuki T, Takada K, Watanabe S, Kawasaki M, Ishizaki H. Taxon- omy of Trichophyton interdigitale (Trichophyton mentagrophytes var. interdigitale) by restriction enzyme analysis of mitochondrial DNA. J Med Vet Mycol. 1990; 28: 191-96.
13. Kawasaki M, Aoki M, Ishizaki H, Mochizuki T, Watanabe S. Phylogen- tic relationships of genera Arthroderma and Nannizzia inferred from mitochondrial DNA analysis. Mycopathologia. 1992; 118: 95-102.
14. Yang G, Zhang M, Li W, An L. Direct species identification of com- mon pathogenic dermatophyte fungi in clinical specimens by semi- nested PCR and restriction fragment length polymorphism. Myco- pathologia. 2008; 166: 203-08.
15. Garg J, Tilak R, Singh S, Gulati AK, Garg A, Prakash P, et al. Evalua- tion of pan-dermatophyte nested PCR in diagnosis of onychomyco- sis. J Clin Microbiol. 2007; 45: 3443-45.
16. Kim JY, Choe YB, Ahn KJ, Lee YW. Identification of dermatophytes using multiplex Polymerase chain reaction. Ann Dermatol. 2011; 23: 304-12.
17. Cafarchia C, Otranto D, Weigl S, Campbell BE, Parisi A, Cantacessi C, et al. Molecular characterization of selected dermatophytes and their identification by electrophoretic mutation scanning. Electropho- resis. 2009; 30: 3555-64.
18. Machouart-Dubach M, Lacroix C, de Chauvin MF, Le Gall I, Giudicelli C, Lorenzo F, et al. Rapid discrimination among dermatophytes, Scytalidium spp., and other fungi with a PCR - restriction fragment length polymor- phism ribotyping method. J Clin Microbiol. 2001; 39: 685-90.
19. Bagyalakshmi R, Senthilvelan B, Therese KL, Murugusundram S, Madhavan HN. Application of polymerase chain reaction (PCR) and PCR based restriction fragment length polymorphism for detection and identification of dermatophytes from dermatological specimens. Indian J Dermatol. 2008; 53: 15-20.
20. Jackson CJ, Barton RC, Evans EG. Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal DNA intergenic spacer regions. J Clin Microbiol. 1999; 37: 931-36.
21. Mochizuki T, Ishizaki H, Barton RC, Moore MK, Jackson CJ, Kelly SL, Evans EG. Restriction fragment length polymorphism analysis of ri- bosomal DNA intergenic regions is useful for differentiating strains of Trichophyton mentagrophytes. J Clin Microbiol. 2003; 41: 4583-88.
22. Ranganathan S, Balajee SA, Menon T. Matting patterns of dermato- phytes of diverse origin in India. Mycopathologia 1996; 136: 91-94.
23. Faergemann J, Baran R. Epidemiology, clinical presentation and diagno- sis of onychomycosis. Br J Dermatol. 2003; 149: 1-4.
24. Weinberg JM, Koestenblatt EK, Tutrone WD, Tishler HR, Najarian L. Comparison of diagnostic methods in the evaluation of onychomy- cosis. J Am Acad dermatol. 2003; 49: 193-97.
25. El Batawi MM, Arnaot H, Shoeib S, Bosseila M, El Fangary M, Helmy AS. The Egyptian Society of Dermatology and Venereology. Prevalence of non dermatophyte molds in patients with abnormal nails. Egyptian Dermatology Online Journal 2006; 2: 1-12. Available at: http://www.edoj.org.eg/vol002/00201/11/001.htm. Accessed June, 2006.