Chlamydia trachomatis clinical isolates identified as tetracycline resistant do not exhibit resistance in vitro: Whole-genome sequencing reveals a mutation in porB but no evidence for tetracycline resistance genes

Microbiology (United Kingdom)

Volumn 159, Issue 4, 2013, Pages 748-756

  O'Neill, C E ^a   Seth Smith, H M B ^b   Van Der Pol, B ^c   Harris, S R ^b   Thomson, N R ^b   Cutcliffe, L T ^a   Clarke, I N ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^c INDIANA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL RNA; RNA 16S; TETRACYCLINE;

ANIMAL CELL; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL MUTATION; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BINDING SITE; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; CULTURE MEDIUM; FLUORESCENCE ANALYSIS; GENE SEQUENCE; GROWTH CURVE; IMMUNOFLUORESCENCE; IN VITRO STUDY; MOLECULAR MECHANICS; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PLASMID; PORB GENE; PRIORITY JOURNAL; STOP CODON;

ANIMALS; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; CELLS, CULTURED; CHLAMYDIA TRACHOMATIS; GENOME, BACTERIAL; HUMANS; MICE; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; MUTATION; PORINS; SEQUENCE ANALYSIS, DNA; TETRACYCLINE; TETRACYCLINE RESISTANCE;

BACTERIA (MICROORGANISMS); CHLAMYDIA TRACHOMATIS;

EID: 84875700825     PISSN: 13500872     EISSN: 14652080     Source Type: Journal    
DOI: 10.1099/mic.0.065391-0     Document Type: Article

References (43)

1. Andersson, S. G., Alsmark, C., Canbäck, B., Davids, W., Frank, C., Karlberg, O., Klasson, L., Antoine-Legault, B., Mira, A. & Tamas, I. (2002). Comparative genomics of microbial pathogens and symbionts. Bioinformatics 18 (Suppl. 2), S17.
2. Assefa, S., Keane, T. M., Otto, T. D., Newbold, C. & Berriman, M. (2009). ABACAS: algorithm-based automatic contiguation of assembled sequences. Bioinformatics 25, 1968-1969.
3. Bordenstein, S. R. & Reznikoff, W. S. (2005). Mobile DNA in obligate intracellular bacteria. Nat Rev Microbiol 3, 688-699.
4. Brodersen, D. E., Clemons, W. M., Jr, Carter, A. P., Morgan-Warren, R. J., Wimberly, B. T. & Ramakrishnan, V. (2000). The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell 103, 1143-1154.
5. Brook, I. (1989). Inoculum effect. Rev Infect Dis 11, 361-368.
6. Carver, T. J., Rutherford, K. M., Berriman, M., Rajandream, M. A., Barrell, B. G. & Parkhill, J. (2005). ACT: the Artemis Comparison Tool. Bioinformatics 21, 3422-3423.
7. Cromwell, G. L. (2002). Why and how antibiotics are used in swine production. Anim Biotechnol 13, 7-27.
8. Donati, M., Di Francesco, A., D'Antuono, A., Delucca, F., Shurdhi, A., Moroni, A., Baldelli, R. & Cevenini, R. (2010). In vitro activities of several antimicrobial agents against recently isolated and genotyped Chlamydia trachomatis urogenital serovars D through K. Antimicrob Agents Chemother 54, 5379-5380.
9. Dugan, J., Rockey, D. D., Jones, L. & Andersen, A. A. (2004). Tetracycline resistance in Chlamydia suis mediated by genomic islands inserted into the chlamydial inv-like gene. Antimicrob Agents Chemother 48, 3989-3995.
10. Enne, V. I. & Bennett, P. M. (2010). Methods to determine antibiotic resistance gene silencing. Methods Mol Biol 642, 29-44.
11. Enne, V. I., Delsol, A. A., Roe, J. M. & Bennett, P. M. (2006). Evidence of antibiotic resistance gene silencing in Escherichia coli. Antimicrob Agents Chemother 50, 3003-3010.
12. Finan, J. E., Rosato, A. E., Dickinson, T. M., Ko, D. & Archer, G. L. (2002). Conversion of oxacillin-resistant staphylococci from heterotypic to homotypic resistance expression. Antimicrob Agents Chemother 46, 24-30.
13. Gerrits, M. M., de Zoete, M. R., Arents, N. L., Kuipers, E. J. & Kusters, J. G. (2002). 16S rRNA mutation-mediated tetracycline resistance in Helicobacter pylori. Antimicrob Agents Chemother 46, 2996-3000.
14. Harris, S. R., Clarke, I. N., Seth-Smith, H. M., Solomon, A. W., Cutcliffe, L. T., Marsh, P., Skilton, R. J., Holland, M. J., Mabey, D. & other authors (2012). Whole-genome analysis of diverse Chlamydia trachomatis strains identifies phylogenetic relationships masked by current clinical typing. Nat Genet 44, 413-419, S1.
15. Jones, R. B., Van der Pol, B., Martin, D. H. & Shepard, M. K. (1990). Partial characterization of Chlamydia trachomatis isolates resistant to multiple antibiotics. J Infect Dis 162, 1309-1315.
16. Kang, C. I., Pai, H., Kim, S. H., Kim, H. B., Kim, E. C., Oh, M. D. & Choe, K. W. (2004). Cefepime and the inoculum effect in tests with Klebsiella pneumoniae producing plasmid-mediated AmpC-type beta-lactamase. J Antimicrob Chemother 54, 1130-1133.
17. Kenny, G. E., Hooton, T. M., Roberts, M. C., Cartwright, F. D. & Hoyt, J. (1989). Susceptibilities of genital mycoplasmas to the newer quinolones as determined by the agar dilution method. Antimicrob Agents Chemother 33, 103-107.
18. Kubo, A. & Stephens, R. S. (2000). Characterization and functional analysis of PorB, a Chlamydia porin and neutralizing target. Mol Microbiol 38, 772-780.
19. Kubo, A. & Stephens, R. S. (2001). Substrate-specific diffusion of select dicarboxylates through Chlamydia trachomatis PorB. Microbiology 147, 3135-3140.
20. LaPlante, K. L. & Rybak, M. J. (2004). Impact of high-inoculum Staphylococcus aureus on the activities of nafcillin, vancomycin, linezolid, and daptomycin, alone and in combination with gentamicin, in an in vitro pharmacodynamic model. Antimicrob Agents Chemother 48, 4665-4672.
21. Lefevre, J. C., Lepargneur, J. P., Guion, D. & Bei, S. (1997). Tetracycline-resistant Chlamydia trachomatis in Toulouse, France. Pathol Biol (Paris) 45, 376-378.
22. Lenart, J., Andersen, A. A. & Rockey, D. D. (2001). Growth and development of tetracycline-resistant Chlamydia suis. Antimicrob Agents Chemother 45, 2198-2203.
23. Milch, R. A., Rall, D. P. & Tobie, J. E. (1958). Fluorescence of tetracycline antibiotics in bone. J Bone Joint Surg Am 40-A, 897-910.
24. Misyurina, O. Y., Chipitsyna, E. V., Finashutina, Y. P., Lazarev, V. N., Akopian, T. A., Savicheva, A. M. & Govorun, V. M. (2004). Mutations in a 23S rRNA gene of Chlamydia trachomatis associated with resistance to macrolides. Antimicrob Agents Chemother 48, 1347- 1349.
25. Pantchev, A., Sting, R., Bauerfeind, R., Tyczka, J. & Sachse, K. (2010). Detection of all Chlamydophila and Chlamydia spp. of veterinary interest using species-specific real-time PCR assays. Comp Immunol Microbiol Infect Dis 33, 473-484.
26. Roblin, P. M. & Hammerschlag, M. R. (2000). In vitro activity of GAR-936 against Chlamydia pneumoniae and Chlamydia trachomatis. Int J Antimicrob Agents 16, 61-63.
27. Ross, J. I., Eady, E. A., Cove, J. H. & Cunliffe, W. J. (1998). 16S rRNA mutation associated with tetracycline resistance in a Gram-positive bacterium. Antimicrob Agents Chemother 42, 1702-1705.
28. Rutherford, K., Parkhill, J., Crook, J., Horsnell, T., Rice, P., Rajandream, M. A. & Barrell, B. (2000). Artemis: sequence visualization and annotation. Bioinformatics 16, 944-945.
29. Sandoz, K. M. & Rockey, D. D. (2010). Antibiotic resistance in Chlamydiae. Future Microbiol 5, 1427-1442.
30. Seth-Smith, H. M. B., Harris, S. R., Persson, K., Marsh, P., Barron, A., Bignell, A., Bjartling, C., Clark, L., Cutcliffe, L. T. & other authors (2009). Co-evolution of genomes and plasmids within Chlamydia trachomatis and the emergence in Sweden of a new variant strain. BMC Genomics 10, 239.
31. Skilton, R. J., Cutcliffe, L. T., Pickett, M. A., Lambden, P. R., Fane, B. A. & Clarke, I. N. (2007). Intracellular parasitism of chlamydiae: specific infectivity of chlamydiaphage Chp2 in Chlamydophila abortus. J Bacteriol 189, 4957-4959.
32. Skilton, R. J., Cutcliffe, L. T., Barlow, D., Wang, Y., Salim, O., Lambden, P. R. & Clarke, I. N. (2009). Penicillin induced persistence in Chlamydia trachomatis: high quality time lapse video analysis of the developmental cycle. PLoS ONE 4, e7723.
33. Solomon, A. W., Mohammed, Z., Massae, P. A., Shao, J. F., Foster, A., Mabey, D. C. & Peeling, R. W. (2005). Impact of mass distribution of azithromycin on the antibiotic susceptibilities of ocular Chlamydia trachomatis. Antimicrob Agents Chemother 49, 4804-4806.
34. Stephens, R. S., Kalman, S., Lammel, C., Fan, J., Marathe, R., Aravind, L., Mitchell, W., Olinger, L., Tatusov, R. L. & other authors (1998). Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis. Science 282, 754-759.
35. Suchland, R. J., Sandoz, K. M., Jeffrey, B. M., Stamm, W. E. & Rockey, D. D. (2009). Horizontal transfer of tetracycline resistance among Chlamydia spp. in vitro. Antimicrob Agents Chemother 53, 4604- 4611.
36. Thomson, N. R., Holden, M. T. G., Carder, C., Lennard, N., Lockey, S. J., Marsh, P., Skipp, P., O'Connor, C. D., Goodhead, I. & other authors (2008). Chlamydia trachomatis: genome sequence analysis of lymphogranuloma venereum isolates. Genome Res 18, 161-171.
37. Unemo, M., Seth-Smith, H. M., Cutcliffe, L. T., Skilton, R. J., Barlow, D., Goulding, D., Persson, K., Harris, S. R., Kelly, A. & other authors (2010). The Swedish new variant of Chlamydia trachomatis: genome sequence, morphology, cell tropism and phenotypic characterization. Microbiology 156, 1394-1404.
38. van Hoek, A. H., Mevius, D., Guerra, B., Mullany, P., Roberts, A. P. & Aarts, H. J. (2011). Acquired antibiotic resistance genes: an overview. Front Microbiol 2, 203.
39. Vester, B. & Douthwaite, S. (2001). Macrolide resistance conferred by base substitutions in 23S rRNA. Antimicrob Agents Chemother 45, 1- 12.
40. Wang, S. A., Papp, J. R., Stamm, W. E., Peeling, R. W., Martin, D. H. & Holmes, K. K. (2005). Evaluation of antimicrobial resistance and treatment failures for Chlamydia trachomatis: a meeting report. J Infect Dis 191, 917-923.
41. Wang, Y., Kahane, S., Cutcliffe, L. T., Skilton, R. J., Lambden, P. R. & Clarke, I. N. (2011). Development of a transformation system for Chlamydia trachomatis: restoration of glycogen biosynthesis by acquisition of a plasmid shuttle vector. PLoS Pathog 7, e1002258.
42. Wyrick, P. B. & Knight, S. T. (2004). Pre-exposure of infected human endometrial epithelial cells to penicillin in vitro renders Chlamydia trachomatis refractory to azithromycin. J Antimicrob Chemother 54, 79-85.
43. Zerbino, D. R. & Birney, E. (2008). Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18, 821- 829.